An improved method for oxidation of chromium(III) oxide-containing fecal samples by using sodium peroxide fusion

Calvert, Richard J.; Kritchevsky, Evelyn S.; Einhorn, E; Klurfeld, David M.; Kritchevsky, David

doi:10.1093/ajcn/49.5.901

Cited by 7 publications

(1 citation statement)

References 6 publications

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“…Unconjugated BA was extracted into diethyl ether and quantitated by gas chromatography/mass spectroscopy as previously described [29,30]. Fecal chromium from the ingested chromium oxide (Cr 2 O 3 ) was determined according to Calvert et al [31]. Concentrations of chromium and acidic sterols in feces 13 were calculated per gram of fecal homogenate.…”

Section: Methodsmentioning

confidence: 99%

Fecal bile acid excretion and messenger RNA expression levels of ileal transporters in high risk gallstone patients

et al. 2009

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BackgroundCholesterol gallstone disease (GS) is highly prevalent among Hispanics and American Indians. In GS, the pool of bile acids (BA) is decreased, suggesting that BA absorption is impaired. In Caucasian GS patients, mRNA levels for ileal BA transporters are decreased. We aimed to determine fecal BA excretion rates, mRNA levels for ileal BA transporter genes and of regulatory genes of BA synthesis in Hispanic GS patients.ResultsExcretion of fecal BA was measured in seven GS females and in ten GS-free individuals, all with a body mass index < 29. Participants ingested the stool marker Cr2O3 (300 mg/day) for 10 days, and fecal specimens were collected on the last 3 days. Chromium was measured by a colorimetric method, and BA was quantitated by gas chromatography/mass spectroscopy. Intake of calories, nutrients, fiber and cholesterol were similar in the GS and GS-free subjects. Mean BA excretion levels were 520 ± 80 mg/day for the GS-free group, and 461 ± 105 mg/day for the GS group. Messenger RNA expression levels were determined by RT-PCR on biopsy samples obtained from ileum during diagnostic colonoscopy (14 GS-free controls and 16 GS patients) and from liver during surgery performed at 8 and 10 AM (12 GS and 10 GS-free patients operated on for gastrointestinal malignancies), all with a body mass index < 29. Messenger RNA level of the BA transporter genes for ileal lipid binding protein, multidrug resistance-associated protein 3, organic solute transporter alpha, and organic solute transporter beta were similar in GS and GS-free subjects. Messenger RNA level of Cyp27A1, encoding the enzyme 27α-hydroxylase, the short heterodimer partner and farnesoid X receptor remained unchanged, whereas the mRNA level of Cyp7A1, the rate limiting step of BA synthesis, was increased more than 400% (p < 0.01) in the liver of GS compared to GS-free subjects.ConclusionHispanics with GS have fecal BA excretion rates and mRNA levels of genes for ileal BA transporters that are similar to GS-free subjects. However, mRNA expression levels of Cyp7A1 are increased in GS, indicating that regulation of BA synthesis is abnormal in Hispanics with GS.

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Section: Methodsmentioning

confidence: 99%