An Improved Method for Cleaning and Preparing Ticks for Examination with the Scanning Electron Microscope

Corwin, Dan; Clifford, Carleton M.; Keirans, James E.

doi:10.1093/jmedent/16.4.352

Cited by 67 publications

(36 citation statements)

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“…-should be selected for SEM, and these should be thoroughly cleaned of any dirt adhering to the integument. Though detergents and ultrasound will remove larger particles (Keirans et al 1976), the most efficient cleaning method presently available is the "glue technique" of Corwin et al (1979). Ticks to be cleaned are removed one at a time from the 70% ethanol in which they are normally stored, placed on a piece of blotting paper approximately 5 x 10 cm in size, and observed under a binocular dissecting microscope while the integument gradually dries.…”

Section: Collecting Preserving and Studying Ticksmentioning

confidence: 99%

The Biological Survey of Canada: A Personal History

Danks¹

2017

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Section: Collecting Preserving and Studying Ticksmentioning

confidence: 99%

The Biological Survey of Canada: A Personal History

Danks¹

2017

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“…In the description, all measurements, first the range and followed by the mean ± the standard deviation in parentheses, are given in millimeters. Representative specimens of larvae and adults were prepared for scanning electron microscopy (SEM) by the method of Corwin et al (1979). The micrographs were obtained using a JEOL JMS-5900 scanning electron microscope.…”

Section: Preparation Of Specimens For Morphological Examinationsmentioning

confidence: 99%

Carios fonsecai sp. nov. (Acari, Argasidae), a bat tick from the central-western region of Brazil

Labruna

Venzal

2009

Acta Parasitologica

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Males, females, and larvae of Carios fonsecai sp. nov. are described from free-living ticks collected in a cave at Bonito, state of Mato Grosso do Sul, Brazil. The presence of cheeks and legs with micromammillate cuticle makes adults of C. fonsecai morphologically related to a group of argasid species (mostly bat-associated) formerly classified into the subgenus Alectorobius, genus Ornithodoros. Examination of larvae indicates that C. fonsecai is clearly distinct from most of the previously described Carios species formerly classified into the subgenus Alectorobius, based primarily on its larger body size, dorsal setae number, dorsal plate shape, and hypostomal morphology. On the other hand, the larva of C. fonsecai is most similar to Carios peropteryx, and Carios peruvianus, from which differences in dorsal plate length and width, tarsal setae, and hypostome characteristics are useful for morphological differentiation. The mitochondrial 16S rDNA sequence of C. fonsecai showed to be closest (85-88% identity) to several corresponding sequences of different Carios species available in GenBank. Bats identified as Peropteryx macrotis and Desmodus rotundus were found infested by C. fonsecai larvae in the same cave where the type series was collected. C. fonsecai showed to be aggressive to humans in the laboratory.

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“…In the description, all measurements, first the range and followed by the mean Ϯ the standard deviation in parentheses, are given in millimeters. Representative specimens were prepared for scanning electron microscopy (SEM) by the method of Corwin et al (1979).…”

Section: Tick Collection and Morphological Studymentioning

confidence: 99%

New Reports of Antricola guglielmonei and Antricola delacruzi in Brazil, and a Description of a New Argasid Species (Acari)

Labruna

Terassini

Camargo

et al. 2008

Journal of Parasitology

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Adults of 3 tick species (Acari: Argasidae) identified as Antricola guglielmonei, Antricola delacruzi, and Carios rondoniensis n. sp. were collected on bat guano in a cave in the state of Rondônia, western Amazon, Brazil. Adults of C. rondoniensis possess a unique combination of characters that distinguish them from all described adults in the Argasidae, i.e., a large spiracular plate densely filled with small goblets, a well-developed flap covering the female genital opening, and palpi containing several tufts of long setae on articles 2 and 3. Unlike Ornithodoros or other Carios species, adults of C. rondoniensis have a scooplike hypostome devoid of denticles, as in Antricola spp. Conversely, the presence of a pair of long posthypostomal setae, and a slitlike transverse fissure at the capsule opening of the Haller's organ, are characters of C. rondonensis that are also found in species of Carios and Ornithodoros, but not in Antricola species. Molecular analyses inferred from a portion of the 16S rRNA mitochondrial gene indicate that C. rondoniensis is phylogenetically closest to species of Carios, followed by species of Antricola, and then Ornithodoros. Because the highest bootstrap value linking C. rondoniensis to Carios spp. was 62%, further phylogenetic studies are needed to better evaluate the taxonomic status of the former species.

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An Improved Method for Cleaning and Preparing Ticks for Examination with the Scanning Electron Microscope

Cited by 67 publications

References 0 publications

The Biological Survey of Canada: A Personal History

The Biological Survey of Canada: A Personal History

Carios fonsecai sp. nov. (Acari, Argasidae), a bat tick from the central-western region of Brazil

New Reports of Antricola guglielmonei and Antricola delacruzi in Brazil, and a Description of a New Argasid Species (Acari)

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