An improved HPLC-UV method for the simultaneous quantification of triterpenic glycosides and aglycones in leaves of Centella asiatica (L.) Urb (APIACEAE)

Rafamantanana, Mamy; Rozet, Eric; Raoelison, Guy; Cheuk, Kiban; Ratsimamanga, Suzanne; Hubert, Ph.; Quetin-Leclercq, Joëlle

doi:10.1016/j.jchromb.2009.03.018

Cited by 99 publications

(60 citation statements)

References 19 publications

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“…HPLC analysis of pentacyclic triterpene content was modified from previous reports (Inamdar et al, 1996;Rafamantanana et al, 2009) and validated. The method was carried out using an Agilent 1100 series equipped with a photodiode array detector and auto sampler (Palo Alto, CA, USA).…”

Section: Hplc Quantitative Analysismentioning

confidence: 99%

Factors affecting the content of pentacyclic triterpenes inCentella asiaticaraw materials

Puttarak

Panichayupakaranant²

2012

Pharmaceutical Biology

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Section: Hplc Quantitative Analysismentioning

confidence: 99%

Factors affecting the content of pentacyclic triterpenes inCentella asiaticaraw materials

Puttarak

Panichayupakaranant²

2012

Pharmaceutical Biology

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“…; particle size 5 lm) with a Perkin-Elmer HPLC system. The mobile phase, consisting of acetonitrile (A) and 0.05% phosphoric acid in water (B), was modified from the protocol described by Rafamantanana et al (2009). The gradients of solvent A were varied as follows: 20-35% A (15 min), 35-65% A (10 min), 65-80% A (5 min), 80-20% A (5 min), and 20% A (5 min) with a flow rate of 0.8 ml min -1 .…”

Section: Hplc Conditionsmentioning

confidence: 99%

In vitro induction of polyploidy in Centella asiatica (L.) Urban

Kaensaksiri

Soontornchainaksaeng

Soonthornchareonnon

et al. 2011

Plant Cell Tiss Organ Cult

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Mutational breeding was conducted using in vitro-grown shoot-tips of Centella asiatica (L.) Urban treated with colchicine (0.025-0.400% for 12-36 h) to induce polyploidy. Treated shoot-tips were grown on Murashige and Skoog (MS) medium supplemented with 4.54 lM thidiazuron (TDZ), and regenerated plantlets were acclimatized and transferred to soil. Two months following transfer to ex vitro conditions, ploidy levels of regenerated plants were determined by flow cytometry and by determining chromosome counts. Treating shoot-tips with colchicine concentrations ranging from 0.050-0.200% for 12-24 h promoted induction of tetraploids. Morphological and growth characteristics and the triterpenoid contents of the polyploids were also measured. Tetraploid plants demonstrated significantly longer stomata and a higher stomatal index compared to those of the diploid control plants. Furthermore, a positive trend in both biomass and triterpenoid production was obtained with the tetraploid and mixoploid plants of C. asiatica.

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“…Numerous reports have been published on the quantification of major triterpenoids from C. asiatica (asiaticoside, madecassoside, asiatic acid, madecassic acid and their isomers) using HPLC (Gunther and Wagner, 1996;Schaneberg et al, 2003;Rafamantanana et al, 2009). However, there has been only a few reports on the identification of C. asiatica constituents using LCMS (Shen et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Chemical characterization and antioxidant activity of three medicinal Apiaceae species

Maulidiani

Abas

Khatib

et al. 2014

Industrial Crops and Products

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a b s t r a c tCentella asiatica ('Pegaga' = Malaysia) is well known plant that has been used as one of the ingredients in pharmaceutical and cosmetic industries. Using liquid chromatography with diode array and electrospray ionization/mass spectrometry (LC-DAD-ESI/MS) analysis, chemical profiling of three different Pegaga extracts (C. asiatica, H. bonariensis and H. sibthorpioides) revealed variations in their metabolite profile. Our findings showed that triterpenes of C. asiatica were characterized by the ursane-type triterpenes (madecassoside, asiaticoside, madecassic acid, and asiatic acid), while the two Hydrocotyle species consisted of oleanane-type triterpenes (barrigenol derivatives). Other variations are due to the difference in phenolic and flavonoid constituents. The three Pegaga extracts were also evaluated for their total phenolic content (TPC), 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radicals, and xanthine oxidase inhibition (XOI) activities. The results showed that C. asiatica has the most potent antioxidant activity (TPC = 72.09 mg/100 g DW; DPPH = 72.99 g/ml; XOI = 87.68 g/ml) as compared to H. bonariensis (TPC = 28.55 mg/100 g DW; DPPH = 22.43 g/ml; XOI = 32.23 g/ml) and H. sibthorpioides (TPC = 56.23 mg/100 g DW; DPPH = 37.86 g/ml; XOI = 14.73. g/ml).

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An improved HPLC-UV method for the simultaneous quantification of triterpenic glycosides and aglycones in leaves of Centella asiatica (L.) Urb (APIACEAE)

Cited by 99 publications

References 19 publications

Factors affecting the content of pentacyclic triterpenes inCentella asiaticaraw materials

Factors affecting the content of pentacyclic triterpenes inCentella asiaticaraw materials

In vitro induction of polyploidy in Centella asiatica (L.) Urban

Chemical characterization and antioxidant activity of three medicinal Apiaceae species

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