An improved, highly sensitive method for the determination of serum iron using chromazurol B

Tabacco, Alessandro; Moda, Edoardo; Tarli, P; Neri, Paolo

doi:10.1016/0009-8981(81)90404-6

Cited by 10 publications

(4 citation statements)

References 2 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This solution turns blue violet at pH 13 (595 nm). It is a highly sensitive colorimetric reagent for Fe(III) and is utilized for the determination of the iron content in blood serum at pH 4.4-5.5 in the presence of cetyltrimethylammonium bromide [33,34]. The dye has been utilized for spectrophotometric determination of protein (human serum albumin) using Chrom Azurol B-beryllium(II) complex by manual and flowinjection methods [35].…”

Section: Dynamic Sorption Of U(vi)mentioning

confidence: 99%

Solid‐Phase Extraction of Trace Amounts of Uranium(VI) in Environmental Water Samples Using an Extractant‐Impregnated Resin Followed by Detection with UV‐Vis Spectrophotometry

Hosseini-Bandegharaei

Sarwghadi

Heydarbeigi

et al. 2013

Journal of Chemistry

View full text Add to dashboard Cite

A stable extractant-impregnated resin (EIR) containing Chrome Azurol B was prepared using Amberlite XAD-2010 as a porous polymeric support. The new EIR was employed for trace separation and preconcentration of U(VI) ion followed by spectrophotometric determination with the arsenazo III procedure. CAB/XAD-2010 exhibited excellent selectivity for U(VI) ion over coexisting ions. Experimental parameters including pH, contact time, shaking speed, and ionic strength were investigated by batch extraction methods. Maximum sorption of U(VI) ions occurred at pH 4.3–6.9. The capacity of EIR was found to be 0.632 mmol·g−1. Equilibrium was reached in 25 min and the loading half-time,t1/2, was less than 6 min. The equilibrium adsorption isotherm of U(VI) was fitted with the Langmuir adsorption model. In addition, a column packed with CAB/XAD-2010 was used for column-mode separation and preconcentration of U(VI) ion. For the optimization of the dynamic procedure, effects of sample volume, sample and eluent flow rate, eluent concentration, and its volume were investigated. The preconcentration factors for U(VI) were found out to be 160. But, for convenience, a preconcentration factor of 150 was utilized for the column-mode preconcentration. The dynamic procedure gave a detection limit of5.0×10-10 mol·L−1(0.12 μg·L−1) for U(VI) ion. The proposed dynamic method showed good performance in analyzing environmental water samples.

show abstract

Section: Dynamic Sorption Of U(vi)mentioning

confidence: 99%

Solid‐Phase Extraction of Trace Amounts of Uranium(VI) in Environmental Water Samples Using an Extractant‐Impregnated Resin Followed by Detection with UV‐Vis Spectrophotometry

Hosseini-Bandegharaei

Sarwghadi

Heydarbeigi

et al. 2013

Journal of Chemistry

View full text Add to dashboard Cite

show abstract

“…The CRX Iron Kit is based on the use of chromazurol B, which creates a complex with Fe 3+ for the direct determination of the labile iron in a buffered acidic environment . No reduction step is needed before the interaction between sample and reagent.…”

Section: Discussionmentioning

confidence: 99%

Iron sucrose: assessing the similarity between the originator drug and its intended copies

Francesco

Philipp

Borchard

2017

Annals of the New York Academy of Sciences

View full text Add to dashboard Cite

Iron sucrose (IS) is a complex nanocolloidal intravenous suspension used in the treatment of iron-deficiency anemia. Follow-on IS products (iron sucrose similars (ISSs)) have obtained marketing authorization by the generic pathway, implying that identical copies of IS may be manufactured. However, recent prospective and retrospective clinical studies showed discrepancies in clinical outcomes, which might be related to differences in physicochemical properties. The aim of this work is to measure and compare the physicochemical properties of IS and three ISSs available in the market using innovative analytical procedures. The comprehensive elucidation of size, size distribution, morphology, and stability of these complex drugs revealed very significant differences between the products. This study serves to provide the basis to define critical quality attributes that may be linked to differences in clinical outcome and thus may contribute to an adequate regulatory approach for IS and its follow-on products.

show abstract

“…In our methodology, for the quantification of zinc in serum, we employed a spectrophotometric determination method facilitated by an Elabscience Biotechnology Kit (Tokimoto et al, 2003), whereas iron concentrations were measured using a kit from QUÍMICA CLÍNICA APLICADA S.A. ( Tobacco et al, 1981) , employing a similar spectrophotometric approach. Vitamin D levels were assessed through the Cobas e 411 analyzer, which leverages electrochemiluminescence (ECL) technology for immunoassay analyses, another innovation from Roche Diagnostics Ltd. (Roach Diagnostic company, Forrenstrasse Rotkreuz, Switzerland).…”

Section: Methods For Determinationsmentioning

confidence: 99%

Investigating Zinc , Iron and 25-hydroxyvitamin D Levels alongside Hemoglobin status in Acute Myocardial Infarction (AMI) Patients

Hassan,

Abdalla,

Gad

et al. 2024

Aswan University Journal of Environmental Studies

View full text Add to dashboard Cite

An improved, highly sensitive method for the determination of serum iron using chromazurol B

Cited by 10 publications

References 2 publications

Solid‐Phase Extraction of Trace Amounts of Uranium(VI) in Environmental Water Samples Using an Extractant‐Impregnated Resin Followed by Detection with UV‐Vis Spectrophotometry

Solid‐Phase Extraction of Trace Amounts of Uranium(VI) in Environmental Water Samples Using an Extractant‐Impregnated Resin Followed by Detection with UV‐Vis Spectrophotometry

Iron sucrose: assessing the similarity between the originator drug and its intended copies

Investigating Zinc , Iron and 25-hydroxyvitamin D Levels alongside Hemoglobin status in Acute Myocardial Infarction (AMI) Patients

Contact Info

Product

Resources

About