An improved high performance liquid chromatographic method for the quantification of solasodine

Kittipongpatana, Nisit; Porter, John R.; Hock, Rick S.

doi:10.1002/(sici)1099-1565(199901/02)10:1<26::aid-pca432>3.0.co;2-5

Cited by 26 publications

(19 citation statements)

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“…[3 -5] Due to the difficulty in the practical separation of all these compounds for rapid analysis, [27] sometimes researchers in this field base their determinations solely on the hydrolysis products and not the actual glycoalkaloids (SGAs); moreover, the aglycones can further hydrolyze, making such determinations of aglycone "equivalents" inaccurate. [28] Methods for the analysis of glycoalkaloids have primarily focused on the potato. [29 -41] Exceptions to this are seen, for example, in the works of Aubert et al who used HPLC for the the analysis of glycoalkaloids present in the eggplant variety Solanum melongena [42,43] and Fukuhara et al who used countercurrent chromatography for the separation of glycoalkaloids in Solanum incanum.…”

Section: Introductionmentioning

confidence: 99%

Development of Practical HPLC Methods for the Separation and Determination of Eggplant Steroidal Glycoalkaloids and their Aglycones

Eanes

Tek

Kirsoy

et al. 2008

Journal of Liquid Chromatography & Related Technologies

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A practical set of HPLC methods was developed for the separation and determination of the eggplant steroidal glycoalkaloids, solanine, chaconine, solasonine, solamargine, and their aglycones, solasodine and solanidine. A gradient method was initially developed, but proved to be neither robust nor practical. Three separate isocratic methods using acetonitrile and ammonium dihydrogen phosphate were developed and shown to be more repeatable, less subject to fluctuations in mobile phase composition, and less time consuming. The effect of adjusting buffer pH, column temperature, and buffer type (triethylammonium phosphate vs. ammonium dihydrogen phosphate) were evaluated. It was also discovered that, by addition of 10% methanol to the acetonitrile portion of the mobile phase, more control over the separations was possible. The use of methanol as a mobile phase entrainer greatly improved separations in some cases and its effectiveness was also dependent upon column temperature. Assessments of the method recovery, limit of detection, and limit of quantitation were made using extracts from S. melongena and S. linnaeanum.

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Section: Introductionmentioning

confidence: 99%

Development of Practical HPLC Methods for the Separation and Determination of Eggplant Steroidal Glycoalkaloids and their Aglycones

Eanes

Tek

Kirsoy

et al. 2008

Journal of Liquid Chromatography & Related Technologies

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“…Extensive literature survey revealed that few HPTLC [5][6][7] and HPLC [8,9] methods are available for estimation of Glycyrrhetic acid individually and in combination with other marker compounds in the same plant. HPTLC [10,11] and HPLC [12,13] methods have also been reported for estimation of Glycyrrhetic acid individually and in combination with other marker compounds. Even though both of these compounds are most commonly used together, there were no reports found for simultaneous estimation of glycyrrhetic acid and solasodine.…”

Section: Introductionmentioning

confidence: 99%

Development and Validation of a HPTLC Method for Simultaneous Densitometric Analysis of Glycyrrhetic Acid and Solasodine in Herbal Drug Formulation

Patel

Bhatt

2017

Int J Curr Pharm Sci

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Objective: To develop and validate a simple, precise, selective, and accurate high-performance thin layer chromatographic method for simultaneous densitometric analysis of glycyrrhetic acid and solasodine in the polyherbal formulation. Methods:The method was developed using HPTLC silica gel GF254precoated aluminium plate as the stationary phase and Chloroform: Methanol (9:1 v/v) as the mobile phase. Quantization of glycyrrhetic acid was achieved by determining the area under the curve at 267 nm using CAMAG TLC Scanner and CATS 3 software. Since the structure of solasodine lacks conjugated double bond, it does not give any fluorescence either in the absorbance mode or reflectance mode hence solasodine was derivatized using 0.5% anisaldehyde sulphuric acids which gave a bluish spot as seen on TLC plate. These spots were scanned at 546 nm wavelength using CAMAG TLC Scanner and CATS 3 software. Results:The retention factor for glycyrrhetic acid and solasodine were found to be 0.52±0.01, 0.40±0.01% w/w respectively. The developed HPTLC method was validated using parameters described in International Conference on Harmonization (ICH) guideline. The proposed method showed good linearity in the range of 400-2000 ng spot -1 for glycyrrhetic acid as well as for solasodine. The content of glycyrrhetic acid and solasodine in marketed polyherbal formulation were found to be 0.67%±0.8 and 0.10±0.35%w/w respectively. Conclusion:The developed method can be used as quality control tool for the routine analysis of glycyrrhetic acid and solasodine in polyherbal formulation.

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“…Solasodine and tomatidine are glycoalkaloids found in solanaceous plants and are used for skin cancer and cancer chemotherapy, respectively [16]. In many countries, solasodine is used as a precursor of steroidal drugs, for example, as an alternative to diosgenin [17].…”

Section: Introductionmentioning

confidence: 99%

Glycoalkaloid isolation fromSolanum linnaeanumberries

Gürbüz

Karabey

Ozturk

et al. 2015

Fruits

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-Introduction. Glycoalkaloids are plant secondary metabolites that can be both harmful and beneficial to human health. They cause gastroenteric symptoms, coma and even death at high concentrations. It is thought that glycoalkaloids are toxic to human health as a result of their effects on the nervous system and destruction of cell membranes. On the other hand, glycoalkaloids can be effective drugs. For example, solasodine is used to treat skin cancer and tomatidine is used in cancer chemotherapy. Solasodine is also used as a precursor of steroidal drugs. The goal of the work was to isolate and separate efficiently these similar compounds. Materials and methods. Glycoalkaloids from Solanum linnaeanum berries were isolated using column chromatography and confirmed via NMR spectroscopy and MS/MS spectrometry. Results and discussion. The chemical structures of glycoalkaloids are nearly identical. For example, solamargine differs from solasonine in having a methyl group instead of a hydroxyl group bound to the sugar residue of the compound. Thus, their molecular weight and polarity are quite similar. In this work, the process yielded 37.1 mg solasonine, 92.3 mg solasonine and solamargine mix and 56.2 mg solamargine from 81.67 g dried S. linnaeanum berries. Conclusion. Solanum linnaeanum berries are a good source of these glycoalkaloids and the developed protocol proved efficient for purification of solasonine and solamargine.Keywords: Turkey / nightshade / Solanum linnaeanum / glycoalkaloids / solamargine / solasonine Résumé -Isolement de glycoalcaloïdes des baies de Solanum linnaeanum L.. Introduction. Les glycoalcaloïdes sont des métabolites secondaires végétaux qui peuvent être à la fois néfastes et bénéfiques pour la santé humaine. À des concentrations élevées, ils provoquent des symptômes de gastro-entérite, le coma et même la mort. On pense que les glycoalcaloïdes sont toxiques pour la santé humaine en raison de leurs effets sur le système nerveux et sur la destruction des membranes cellulaires. D'autre part, les glycoalcaloïdes peuvent être des médicaments efficaces. Par exemple, la solasodine est utilisée pour traiter le cancer de la peau et la tomatidine est utilisée en chimiothérapie du cancer. La solasodine est également utilisée comme précurseur de médicaments stéroïdiens. Le but de ce travail consistait à isoler et séparer efficacement ces composés analogues. Matériels et méthodes. Les glycoalcaloïdes extraits des baies de morelle de Linné (Solanum linnaeanum) ont été isolés par chromatographie sur colonne et leurs structures confirmées par spectroscopie à résonance magnétique nucléaire couplée à la spectrométrie de masse en tandem (RMNMS/MS). Résultats et discussion. Les structures chimiques des glycoalcaloïdes se sont révélées quasi identiques. La solasonine s'est distinguée de la solamargine par un groupe méthyle à la place d'un groupe hydroxyle lié au résidu carbohydrate du composé. Ainsi, le poids moléculaire et la polarité de ces deux composés sont assez semblables. Dans ce travail, le pro...

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An improved high performance liquid chromatographic method for the quantification of solasodine

Cited by 26 publications

References 9 publications

Development of Practical HPLC Methods for the Separation and Determination of Eggplant Steroidal Glycoalkaloids and their Aglycones

Development of Practical HPLC Methods for the Separation and Determination of Eggplant Steroidal Glycoalkaloids and their Aglycones

Development and Validation of a HPTLC Method for Simultaneous Densitometric Analysis of Glycyrrhetic Acid and Solasodine in Herbal Drug Formulation

Glycoalkaloid isolation fromSolanum linnaeanumberries

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