An improved high-performance liquid chromatography (HPLC) method for detection of variations in the hydroxyproline content of tissue homogenates from Paracoccidioides brasiliensis-infected mice
Magnus Ake Gidlund,
Raphael Fagnani Sanchez Molina,
Eva Burger
Abstract:Background:
Paracoccidioidomycosis (PCM) is a severe granulomatous
disease
.
The hallmark of this mycosis is fibrin
degradation and granuloma formation as a result of a wound-healing process
in the context of excessive inflammation. Therefore, as the content of
collagen can be assessed by the methodology described in this manuscript, we
propose that the content of hydroxyproline (HYP) be employed as a new and
efficient measurement of granulomatous lesions developed. To e… Show more
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