An improved glucose-oxidase method for determining blood, C.S.F. and urine glucose levels

Маркс,

doi:10.1016/0009-8981(96)83704-1

Cited by 41 publications

(35 citation statements)

References 9 publications

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“…Plasma obtained by centrifugation (1600 g for 10 min at 4°C) was used for measurements of plasma glucose, glucosylated haemoglobin (HbA 1c ) and insulin levels. Plasma glucose was measured by the glucose‐oxidative method (Marks 1959). Glucosylated haemoglobin was determined using the sodium lauryl sulphate–haemoglobin (SLS–Hb) method (Falco Biosystems, Kyoto, Japan).…”

Section: Methodsmentioning

confidence: 99%

Fibre type distribution and gene expression levels of both succinate dehydrogenase and peroxisome proliferator‐activated receptor‐γ coactivator‐1α of fibres in the soleus muscle of Zucker diabetic fatty rats

Adachi

Kikuchi²,

Yasuda³

et al. 2007

Experimental Physiology

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We have reported that a change in muscle fibre type distribution is present in two strains of diabetic rats (Otsuka Long-Evans Tokushima Fatty and Goto-Kakizaki rats). In this study, we determined whether the change in soleus muscle fibre type distribution was caused by diabetes, using obese, diabetic (Zucker diabetic fatty, ZDF), obese, non-diabetic (Zucker fatty, ZF) and non-diabetic, non-obese rats (Zucker lean, ZL). Moreover, we investigated whether the gene expression levels of metabolic key molecules, namely the transcriptional factors of metabolic genes, exemplified by peroxisome proliferator-activated receptor-γ coactivator-1α (PGC-1α), and the oxidative enzymes in mitochondria, exemplified by succinate dehydrogenase (SDH), were changed in type I and II muscle fibres in each type of rat, using the new technique of laser capture microdissection (LCM). Both plasma glucose and glucosylated haemoglobin levels were significantly higher in ZDF than in ZL and ZF rats. A lower percentage of type IIA fibres was observed in the muscles of ZDF rats than in those of ZL and ZF rats. The mRNA expression levels of SDH in type II fibres and of PGC-1α in type I fibres were significantly lower in ZDF than in ZL and ZF rats as assessed by LCM and real-time PCR analysis. We have shown, for the first time, that a lower percentage of type IIA fibres was observed in ZDF rats. We have also discovered that the expression levels of the oxidative metabolism-related genes, PGC-1α and SDH, decreased in type I and type II fibres, respectively, of ZDF rats.

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Section: Methodsmentioning

confidence: 99%

Fibre type distribution and gene expression levels of both succinate dehydrogenase and peroxisome proliferator‐activated receptor‐γ coactivator‐1α of fibres in the soleus muscle of Zucker diabetic fatty rats

Adachi

Kikuchi²,

Yasuda³

et al. 2007

Experimental Physiology

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“…3 It was almost a quarter of a century before this conceptual terminology could be validated, courtesy of the advent of insulin radioimmunoassay and improved methodologies for accurate glucose measurement, 4,5 and intense research has ensued.…”

Section: Insulin Resistancementioning

confidence: 99%

Metabolic syndrome, or What you will: definitions and epidemiology

Day

2007

Diabetes and Vascular Disease Research

242

189

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The metabolic syndrome is a clustering of risk factors which predispose an individual to cardiovascular morbidity and mortality. There is general consensus regarding the main components of the syndrome (glucose intolerance, obesity, raised blood pressure and dyslipidaemia [elevated triglycerides, low levels of high-density lipoprotein cholesterol]) but different definitions require different cut points and have different mandatory inclusion criteria. Although insulin resistance is considered a major pathological influence, only the World Health Organization (WHO) and European Group for the study of Insulin Resistance (EGIR) definitions include it amongst the diagnostic criteria and only the International Diabetes Federation (IDF) definition has waist circumference as a mandatory component. The prevalence of metabolic syndrome within individual cohorts varies with the definition used. Within each definition, the prevalence of metabolic syndrome increases with age and varies with gender and ethnicity. There is a lack of diagnostic concordance between different definitions. Only about 30% of people could be given the diagnosis of metabolic syndrome using most definitions, and about 3540% of people diagnosed with metabolic syndrome are only classified as such using one definition. There is currently debate regarding the validity of the term metabolic syndrome, but the presence of one cardiovascular risk factor should raise suspicion that additional risk factors may also be present and encourage investigation. Individual risk factors should be treated.

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“…Two-hour post-prandial plasma glucose concentrations were also determined after a brief breakfast meal that was comprised of ~300 kcal cake (of the same brand in all participants) and a cup of tea. Glucose measurements (intra-assay coefficient of variants [CV] 2.1%, inter-assay CV 2.6%) were carried out using the glucose oxidase method (16). Chl, TG, and HDL-C were determined using enzymatic methods (Parsazmun, Karaj, Iran).…”

Section: Blood Samplesmentioning

confidence: 99%

HOMA-Estimated Insulin Resistance Is Associated with Hypertension in Iranian Diabetic and Non-Diabetic Subjects

Esteghamati¹,

Khalilzadeh²,

Abbasi³

et al. 2008

Clinical and Experimental Hypertension

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The relationship between insulin resistance (IR) and essential hypertension (HTN) is controversial. The aim of this study was to determine the association of IR estimated by homeostasis model assessment of insulin resistance (HOMA-IR) and HTN in a large sample of Iranian diabetic and non-diabetic population. A total of 2047 diabetic and non-diabetic individuals with or without HTN, aged 30-75 yrs, who were referred to a university general hospital between November 2004 and April 2007 were included in this study. Demographic data and anthropometric characteristics of participants were recorded. Fasting blood samples were collected, and fasting plasma glucose (FPG), serum creatinine, lipids, insulin, C-peptide and HbA1c were measured. HOMA-IR and HOMA derived Beta-cell function (HOMA-B) were also calculated. Age, sex and waist girth adjusted HOMA-IR values were compared between hypertensive and normotensive subjects. Hypertensive patients had significantly higher HOMA-IR than age-, sex-, and waist girth-adjusted normotensive individuals in both non-diabetic (2.163 +/- 0.08 and 1.75 +/- 0.03, p < 0.001) and diabetic (3.40 +/- 0.10 and 3.07 +/- 0.09, p < 0.05) groups. Multivariate logistic regression analysis showed that after adjustment for age, sex, waist girth, BMI, triglyceride, total cholesterol, FPG, and C-peptide, HOMA-IR was a significant independent predictor of HTN in all subjects (odds ratio = 1.117, CI 95% = 1.026-1.216, p < 0.05) and in diabetic and non-diabetic subjects separately (odds ratio = 1.102, CI 95% = 1.009-1.203, p < 0.05 and odds ratio = 1.328, CI 95% = 1.116-1.580, p < 0.01, respectively). In conclusion, this study showed that IR is associated with HTN in Iranian diabetic and non-diabetic subjects.

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An improved glucose-oxidase method for determining blood, C.S.F. and urine glucose levels

Cited by 41 publications

References 9 publications

Fibre type distribution and gene expression levels of both succinate dehydrogenase and peroxisome proliferator‐activated receptor‐γ coactivator‐1α of fibres in the soleus muscle of Zucker diabetic fatty rats

Fibre type distribution and gene expression levels of both succinate dehydrogenase and peroxisome proliferator‐activated receptor‐γ coactivator‐1α of fibres in the soleus muscle of Zucker diabetic fatty rats

Metabolic syndrome, or What you will: definitions and epidemiology

HOMA-Estimated Insulin Resistance Is Associated with Hypertension in Iranian Diabetic and Non-Diabetic Subjects

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