An improved estrogenic activity reporter gene assay (T47D-KBluc) for detecting estrogenic activity in wastewater and drinking water

Chou, Hui-Ming; Chao, How‐Ran; Lin, Ching‐Yi; Chiang, Pen‐Chi; Wang, Gen-Shuh; Tsou, Tsui‐Chun

doi:10.1080/02772248.2015.1123481

Cited by 6 publications

(6 citation statements)

References 23 publications

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“…Estrogenic activity has been widely studied in treated drinking water using a range of in vitro assays [11][12][13][14][21][22][23][24][25], with an overview of reported activity provided in Figure 2. Many studies also measure estrogenic activity in source water and compare BEQ bio before and after treatment, with 39 to 99% removal of estrogenic activity reported [12,13,[23][24][25], which indicates the removal or degradation of causative compounds.…”

Section: Application Of In Vitro Bioassays To Drinking Watermentioning

confidence: 99%

In vitro bioassays to assess drinking water quality

Neale

Escher

2019

Current Opinion in Environmental Science & Health

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In vitro assays indicative of different stages of cellular toxicity pathways have been applied to both source water and drinking water. The majority of studies showed a decrease in receptor-mediated effects after drinking water treatment due to the removal of micropollutants, while reactive toxicity typically increased after chlorination due to the formation of disinfection byproducts. Using both chemical analysis and bioanalysis, iceberg modelling can be applied to determine which chemicals are contributing to the observed effect, though one limitation is that typical sample pretreatment for bioanalysis fails to capture volatile chemicals. Bioassays are increasingly sensitive and effects can be detected in clean samples, thus effect-based trigger values can be applied to determine whether an effect in drinking water is acceptable.

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Section: Application Of In Vitro Bioassays To Drinking Watermentioning

confidence: 99%

In vitro bioassays to assess drinking water quality

Neale

Escher

2019

Current Opinion in Environmental Science & Health

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“…It should, however, be noted that the estrogenic activities observed in these samples are all considerably below this benchmark value of 1 ng E2/L. Previous studies of estrogenic activity in drinking water, using different bioassays based on mammalian cells, have reported BEQ values in the range of 0.01-5 ng E2eq/L [14][15][16][17][18][19][20]. Effect-based trigger values for estrogenic activities in drinking water in the range 0.1-3.8 ng E2eq/L have been proposed [11,15,21,22].…”

Section: Estrogen Receptor Activitymentioning

confidence: 73%

Glass-bottled drinking water: a time capsule to study the historic presence of hazardous chemicals using effect-based methods

2021

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Background Contamination of drinking water by hazardous chemicals can be associated with human health risks. Recent studies using effect-based in vitro methods have demonstrated that a large part of the observed toxic effects are caused by unknown chemicals. In this study, we have used a panel of effect-based methods to study the presence of chemical contaminants in a unique material; glass-bottled Swedish tap water collected during the 1990s. These water samples were compared to drinking water from the same source waters and drinking water facilities, yet collected about 25 years later, in 2020. Results Samples were concentrated by solid phase extraction and evaluated for the following activities; estrogen receptor activity, androgen receptor activity, antiandrogenic activity, aryl hydrocarbon receptor activity, and oxidative stress response. We observed aryl hydrocarbon receptor activities in almost all studied samples and estrogen receptor activity in three out of ten studied samples. No activities were observed for androgen receptor activity, antiandrogenic activity or oxidative stress response. In general, observed activities were more frequent and higher in the water samples collected during the 1990s as compared to the corresponding samples collected in 2020. Conclusions This study demonstrates that it is possible to conduct an effect-based evaluation of the presence of hazardous chemicals in drinking water, with as small starting volume as 330 mL, by using miniaturized bioassays. Further, by comparing the glass-bottled water samples with newly collected water samples from the same drinking water treatment facilities, our results indicate that the presence of aryl hydrocarbon receptor and estrogen receptor activating compounds in the drinking water has decreased over the approximately quarter of a century that is separating the two sampling occasions. This difference could be due to improved raw water quality and/or improved treatment efficiency in the treatment plants.

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“…Therefore, we did similarly to Chou et al, and to improve the estrogen test sensitivity, we included T47D-KBluc cells grown in medium without phenol red for 12h and subsequently exposed the cells to the test substances. Literature data show that such an effect signi cantly stimulates estradiol (E2)-induced activation of luciferase and thus its sensitivity and precision (Chou HM et al, 2016). A similar method, also based on the T47D-KBluc cells, was used by Wehmas et al (Wehmas et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

“…Bioassay for measurement of estrogenic activity An estrogen receptor (ER) reporter gene assay, referred to as the T47D-KBluc assay, was performed according to Chou et al (Chou et al, 2016).…”

Section: E-screen Testmentioning

confidence: 99%

"Selected fungicides as potential EDC estrogenic micropollutants in the environment"

Jabłońska-Trypuć,

Wydro,

Wołejko

et al. 2023

Preprint

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An increasing level of pesticides exposition is being observed as a result of the consumption of large amounts of fruit, vegetables and grain products, which are key components of the vegetarian diet. Fungicides have been classified as a endocrine-disrupting compounds, but their mechanisms of action have not yet been clarified. The effect of boscalid (B), cyprodinil (C) and iprodione (I) combined with Tamoxifen (T) and 17β-estradiol (E2) on cell viability, cell proliferation, reporter gene expression, ROS content, cell membrane’s function, cell morphology and antioxidant enzymes gene expression on MCF-7 and T47D-KBluc cell lines were investigated. Cell lines were chosen due to their response to 17β -estradiol. Selected fungicides are commonly used in Poland to protect crops against fungi. Our results revealed that studied fungicides caused significant increase in cell viability and proliferation and estrogenic activity was present in all studied compounds depending on their concentrations. Oxidative stress activated uncontrolled cancer cell proliferation by inducing ROS production and by inhibiting antioxidant defense. Our findings verified that studied fungicides could possibly exhibit endocrine disrupting properties and exposure should be avoided.

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An improved estrogenic activity reporter gene assay (T47D-KBluc) for detecting estrogenic activity in wastewater and drinking water

Cited by 6 publications

References 23 publications

In vitro bioassays to assess drinking water quality

In vitro bioassays to assess drinking water quality

Glass-bottled drinking water: a time capsule to study the historic presence of hazardous chemicals using effect-based methods

"Selected fungicides as potential EDC estrogenic micropollutants in the environment"

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