An Improved Electrophoretic Method for Identifying Antibiotics with Special Reference to Animal Tissues and Animal Feeding Stuffs

Smither, R.; Vaughan, David R

doi:10.1111/j.1365-2672.1978.tb00816.x

Cited by 27 publications

(13 citation statements)

References 13 publications

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“…Similarly, sample #3 was classified as penicillin (+) by the microbial assay, whereas LC/MS n gave a negative result. These discrepancies between the two techniques could be due to a variety of causes, including poor detection of DCCD at lower concentrations, the presence of non‐ β ‐lactam antibiotics, or the presence of natural microbial inhibitors 22–25…”

Section: Resultsmentioning

confidence: 99%

Confirmatory analysis of β‐lactam antibiotics in kidney tissue by liquid chromatography/electrospray ionization selective reaction monitoring ion trap tandem mass spectrometry

Fagerquist

Lightfield

2003

Rapid Comm Mass Spectrometry

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Eleven beta-lactam antibiotics were analyzed in fortified and incurred beef kidney tissue using high-performance liquid chromatography/electrospray ionization/selective reaction monitoring-ion trap tandem mass spectrometry (LC/ESI-SRM-MS(n)). The analytes included: deacetylcephapirin, amoxicillin, cephapirin, desfuroylceftiofur cysteine disulfide (DCCD, a biomarker of ceftiofur), ampicillin, cefazolin, Pen G, oxacillin, cloxacillin, naficillin and dicloxicillin. Analytes were extracted with acetonitrile and water. Clean-up was performed by solid-phase extraction. Limits of confirmation in fortified tissue are as follows (tolerances or target levels in parentheses): deacetylcephapirin: 10-50 ng/g (100 ng/g); amoxacillin: 50-100 ng/g (10 ng/g); cephapirin: 10 ng/g (100 ng/g); DCCD: 500 ng/g (8000 ng/g); ampicillin: 10 ng/g (10 ng/g); cefazolin: 10 ng/g (10-50 ng/g); Pen G: 10 ng/g (50 ng/g); oxacillin: 10 ng/g (10-50 ng/g); cloxacillin: 10 ng/g (10 ng/g); naficillin: 10 ng/g (10-50 ng/g); dicloxacillin: 100-500 ng/g (10-50 ng/g). The present method was also tested on incurred kidney tissue that had previously been analyzed using a microbial assay. Good correspondence was found between the results from this new method and the bioassay. However, the present method is much more specific and, in several cases, more sensitive than the bioassay. In addition, the time of analysis is significantly shorter than the bioassay. We also found that SRM MS(n) was superior in the analysis of unknown incurred tissue than full spectrum MS(n). We also obtained an MS/MS spectrum of DCCD that is significantly at variance with previously published fragmentation spectra.

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Section: Resultsmentioning

confidence: 99%

Confirmatory analysis of β‐lactam antibiotics in kidney tissue by liquid chromatography/electrospray ionization selective reaction monitoring ion trap tandem mass spectrometry

Fagerquist

Lightfield

2003

Rapid Comm Mass Spectrometry

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“…(1979) for the FPT, and with those of Smither & Vaughan (1978) for electrophoresis. New data are given for several veterinary antibiotics that were not studied by those authors (Tables 1 and 2).…”

Section: Resultsmentioning

confidence: 97%

“…2 with added trimethoprim, and Micrococcus luteus at pH 8.0. The sensitivity of this test to some antibiotics has been evaluated, but the data d o not encompass the full range of agents available for use in animal husbandry in the UK and/or the European Community (Smither & Vaughan 1978;Anon 1980Anon , 1981Bogaerts &Wolf 1980).…”

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confidence: 99%

A note on inhibition test and electrophoretic detection limits of antibiotics used in British animal husbandry

Bates

Lindsay

Watson

1983

Journal of Applied Bacteriology

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A four plate microbiological inhibition test (the FPT) and a bioelectrophoretic method were evaluated for their ability to detect a range of antibiotic agents, which may be present as residues in animal tissues following their therapeutic use in animal husbandry. Both methods exhibited a wide range of sensitivities and several of the tested antibiotics could not be detected by either method. The pattern of responses across the bacterial plates in the FPT could not be used to identify agents and the bioelectrophoretic inhibition zone diameters were generally too large to allow the use of Rs values for identification. The Bacillus subtilis pH 7.2 plate with trimethoprim added was as effective as the four bacterial plates used in the FPT in antibiotic detection.

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“…The present study was undertaken to identify certain naturally produced inhibitors, following a recent survey in which 200 of 789 (25.5%) chicken liver and pig kidney samples, screened for antibiotic activity by the method of Smither (1975), gave a positive result. High voltage electrophoresis (Smither & Vaughan 1978) confirmed that four samples contained antibiotics. This method also showed, however, that the inhibitory activity of the remaining 196 positive samples, which moved towards the anode as an apparently single substance, was common to all the positive samples, but did not behave like any antibiotic encountered previously.…”

mentioning

confidence: 88%

“…A portion of the dry powder (5 g) was extracted with acetonitrile-distilled water (9:1, by vol.) as described by Smither & Vaughan (1978). Portions (50 PI) of the reconstituted extracts were added to wells (10 mm diam.…”

Section: Determination Of Total Inhibitionmentioning

confidence: 99%

Bacterial Inhibitors Formed During the Adventitious Growth of Micro‐organisms in Chicken Liver and Pig Kidney

Smither¹

1978

Journal of Applied Bacteriology

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Inhibitory activity (detected by Bacillus cereus var. mycoides), identical with that encountered in survey samples, was induced in homogenized fresh chicken liver or pig kidney samples by incubating at 30°C. relative potency of each of three active components as detected by t.l.c./bio‐autography of extracts of freeze‐dried material was ascertained. Three strains of Streptococcus faecalis and a Lactobacillus sp. were responsible for production of the inhibitory activity, which was not produced by any of these organisms in synthetic liquid media.

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An Improved Electrophoretic Method for Identifying Antibiotics with Special Reference to Animal Tissues and Animal Feeding Stuffs

Cited by 27 publications

References 13 publications

Confirmatory analysis of β‐lactam antibiotics in kidney tissue by liquid chromatography/electrospray ionization selective reaction monitoring ion trap tandem mass spectrometry

Confirmatory analysis of β‐lactam antibiotics in kidney tissue by liquid chromatography/electrospray ionization selective reaction monitoring ion trap tandem mass spectrometry

A note on inhibition test and electrophoretic detection limits of antibiotics used in British animal husbandry

Bacterial Inhibitors Formed During the Adventitious Growth of Micro‐organisms in Chicken Liver and Pig Kidney

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