An improved elastase-based method to create a saccular aneurysm rabbit model

Wang, Yübo; Ma, Chi; Xu, Ning; Xu, Kan; Wang, Honglei; Yu, Jinlu; Yang, Li; Wang, Kuizhong; Wang, Xu; Luo, Qiong

doi:10.3109/02688697.2013.799633

Cited by 8 publications

(4 citation statements)

References 23 publications

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“…[ 123 ] 2013 X Surgical Rabbits Saccular X Wang Y. [ 124 ] 2013 X Surgical Rabbits Saccular X Kühn A.L. [ 125 ] 2014 X Surgical Rabbits Saccular X Simgen A.…”

Section: Methodsmentioning

confidence: 99%

Imaging of intracranial aneurysms in animals: a systematic review of modalities

et al. 2023

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Intracranial aneurysm (IA) animal models are paramount to study IA pathophysiology and to test new endovascular treatments. A number of in vivo imaging modalities are available to characterize IAs at different stages of development in these animal models. This review describes existing in vivo imaging techniques used so far to visualize IAs in animal models. We systematically searched for studies containing in vivo imaging of induced IAs in animal models in PubMed and SPIE Digital library databases between 1 January 1945 and 13 July 2022. A total of 170 studies were retrieved and reviewed in detail, and information on the IA animal model, the objective of the study, and the imaging modality used was collected. A variety of methods to surgically construct or endogenously induce IAs in animals were identified, and 88% of the reviewed studies used surgical methods. The large majority of IA imaging in animals was performed for 4 reasons: basic research for IA models, testing of new IA treatment modalities, research on IA in vivo imaging of IAs, and research on IA pathophysiology. Six different imaging techniques were identified: conventional catheter angiography, computed tomography angiography, magnetic resonance angiography, hemodynamic imaging, optical coherence tomography, and fluorescence imaging. This review presents and discusses the advantages and disadvantages of all in vivo IA imaging techniques used in animal models to help future IA studies finding the most appropriate IA imaging modality and animal model to answer their research question.

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“…[ 123 ] 2013 X Surgical Rabbits Saccular X Wang Y. [ 124 ] 2013 X Surgical Rabbits Saccular X Kühn A.L. [ 125 ] 2014 X Surgical Rabbits Saccular X Simgen A.…”

Section: Methodsmentioning

confidence: 99%

Imaging of intracranial aneurysms in animals: a systematic review of modalities

et al. 2023

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“…The elastase-induced ECA bifurcation stump model also underwent modifications. Wang et al 40 proposed to ligate the ECA temporally using an aneurysm clip and infuse the ECA stump with elastase for 20 min. This surgical procedure is simple, fast, and requires no interventional support.…”

Section: Represents a Natural Arterial Bifurcation (Figure 4 B6; Supmentioning

confidence: 99%

Preclinical extracranial aneurysm models for the study and treatment of brain aneurysms: A systematic review

Marbacher

Strange

Frösén

et al. 2020

J Cereb Blood Flow Metab

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Animal models make an important contribution to our basic understanding of the pathobiology of human brain aneurysms, are indispensable in testing novel treatment approaches, and are essential for training interventional neuroradiologists and neurosurgeons. Researchers are confronted with a broad diversity of models and techniques in various species. This systematic review aims to summarize and categorize extracranial aneurysm models and their characteristics, discuss advantages and disadvantages, and suggest the best use of each model. We searched the electronical Medline/PubMed database between 1950 and 2020 to identify main models and their refinements and technical modifications for creation of extracranial aneurysms. Each study included was assessed for aneurysm-specific characteristics, technical details of aneurysm creation, and histological findings. Among more than 4000 titles and abstracts screened, 473 studies underwent full-text analysis. From those, 68 different techniques/models in five different species were identified, analyzed in detail, and then grouped into one of the five main groups of experimental models as sidewall, terminal, stump, bifurcation, or complex aneurysm models. This systematic review provides a compact guide for investigators in selecting the most appropriate model from a range of techniques to best suit their experimental goals, practical considerations, and laboratory environment.

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“…For DSA, animals were anesthetized and the femoral artery was exposed. Rabbits were prepared and DSA performed as described in published methods to observe the morphology of the aneurysm [12].…”

Section: Visualization Of Aneurysm and Measurements Of Vesselmentioning

confidence: 99%

An Effective and Simple Way to Establish Elastase-Induced Middle Carotid Artery Fusiform Aneurysms in Rabbits

Lyu

Luo

Zhang

et al. 2020

BioMed Research International

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Objective. Elastase-induced aneurysms in rabbits have been proposed as a preclinical tool for device development, but there is still much deficiency in those aneurismal models. So we need to explore the efficient and convenient animal models for the investigation of intracranial aneurysms. Then, we compared and analyzed three methods of elastase-induced carotid artery aneurysms in rabbits and aimed to find a simple, effective, and reproducible method for creating elastase-induced aneurysms. Methods. 42 standard feeding male adult Japanese white rabbits (3.05±0.65 kg) were randomly divided into 3 groups and treated with elastase ablation to create common carotid artery (RCCA) aneurysm models: Group A (root-RCCA medication group, n=12), Group B (mid-RCCA medication group, n=18), and Group C (ligated RCCA+medication group, n=12). For Group A, the origin of the RCCA was blocked by two temporary aneurysm clips, and the resulting 2 cm cavity was infused with elastase for 20 min, then the clip was removed and the RCCA was not ligated. For Group B, the middle part of RCCA was treated the same way as Group A and the RCCA was not ligated. For Group C, the middle part of RCCA was treated as Group B, but the distal RCCA was ligated. After the aneurysm models were created for 3 weeks, prior to sacrificing the animals, color Doppler ultrasound and angiography were performed for blood flow measurements inside the aneurysms. Histological analysis (such as SMA-α, CD31, CD34, CD68, collagen IV, and Ki67) and the other relevant indexes were compared between the ideal model’s aneurysmal tissues and the human intracranial aneurysm’s tissues to confirm whether we have successfully established elastase-induced aneurysm models. Results. Compared with human intracranial aneurysm specimens by the color Doppler ultrasound, angiography, and changes in the inner diameter of arteries, all three methods have successfully established the elastase-induced aneurysm models. Histology showed that biological responses were similar to both human cerebral aneurysms and previously published elastase-induced rabbit aneurysm models. Group A and Group B had the same morphology, but Group A had a higher mortality rate than Group B. Group B and Group C had different morphology. The aneurysm of Group C was more similar to human cerebral aneurysms but had a higher mortality rate than Group B. Group B was confirmed not only as an alternative method but also as a more safe and effective method for creating elastase-induced aneurysm models. Conclusion. Through analysis and comparison, the Group B is proven to be the simplest, reproducible, and most effective modeling method. The aneurysm model established by Group B can be used for basic research related to aneurysm mechanism. We have provided a new and effective method for basic research on aneurysm.

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An improved elastase-based method to create a saccular aneurysm rabbit model

Cited by 8 publications

References 23 publications

Imaging of intracranial aneurysms in animals: a systematic review of modalities

Imaging of intracranial aneurysms in animals: a systematic review of modalities

Preclinical extracranial aneurysm models for the study and treatment of brain aneurysms: A systematic review

An Effective and Simple Way to Establish Elastase-Induced Middle Carotid Artery Fusiform Aneurysms in Rabbits

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