An Improved Diffusion Chamber (DC) for Culture of Hematopoietic Cells

Carsten, A. L.; Chanana, A. D.; Chikkappa, G.; Cronkite, Eugene P.; Öhl, S.

doi:10.3181/00379727-150-38983

Cited by 10 publications

(3 citation statements)

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“…For almost 15 years, several diffusion chambers have been widely used to encase implanted tissues, including thyroid (25), thymus (26), and ovarian (27) tissues and hemopoietic cells (28). Chambers constructed with Millipore filters have been used in pancreatic islet implants in studies on obese mice (29,30) while Maratos et al (31) described a diffusion chamber constructed with nucleopore filters.…”

Section: Discussionmentioning

confidence: 99%

Membrane immunoisolation of a diffusion chamber for a bioartificial pancreas

Ohgawara¹,

Hirotani²,

Edamura³

et al. 2000

Diabetes Research and Clinical Practice

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Section: Discussionmentioning

confidence: 99%

Membrane immunoisolation of a diffusion chamber for a bioartificial pancreas

Ohgawara¹,

Hirotani²,

Edamura³

et al. 2000

Diabetes Research and Clinical Practice

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“…For almost 15 years, several diffusion chambers have been widely used to encase implanted tissues, including thyroid ( 25), thymus ( 26), and ovarian ( 27) tissues and hemopoietic cells ( 28). Chambers constructed with Millipore filters have been used in pancreatic islet implants in studies on obese mice ( 29, 30) while Maratos et al.…”

Section: Discussionmentioning

confidence: 99%

Membrane Immunoisolation of a Diffusion Chamber for a Bioartificial Pancreas

et al. 1998

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Immunoisolation is a potentially important approach to transplanting islets without any immunosuppressive therapy. The concept of immunoisolation is outlined in systems in which the transplanted tissue is separated from the immune system of the host by an artificial barrier. We previously described a diffusion chamber as a bioartificial endocrine pancreas (Bio-AEP), which was constructed by placing pancreatic endocrine cells, trapped in a mixed matrix, in the center of a ring holder sandwiched between nucleopore membranes, which were shielded by silicone. This experiment was designed to evaluate a suitable pore size for the nucleopore membrane to ensure immunoisolation during xenoimplantation of the Bio-AEP in vitro and in vivo. A nucleopore membrane of pore size 0.1 microm or 0.2 microm was employed as the semipermeable membrane which provided a mechanical barrier between the endocrine pancreas graft and the host immune system. The protective effect of the Bio-AEP from humoral immunity was determined in vitro, using sensitized sheep erythrocytes (EAs). A complement protein did not destroy the cell membranes of the EAs in the diffusion chamber containing the mixed matrix with the nucleopore membrane of 0.1 microm pore size. In an in vivo experiment, 6 streptozotocin (STZ) induced diabetic rats were implanted with Bio-AEPs constructed with nucleopore membranes of pore size 0.1 microm and containing MIN6 cells in the mixed matrix. In the STZ diabetic rats with Bio-AEPs, a return to normoglycemia was observed up to 50 weeks after implantation without the use of any immunosuppressant. Also, the body weights of the rats gradually increased. During the observation, when the Bio-AEPs were removed from the STZ diabetic rats, the blood glucose immediately returned to preimplantation levels, and the body weights of the rats also decreased. The membranes of the Bio-AEPs removed from the STZ diabetic rats showed a very thin layer of fibroblastic cells on the outer surfaces. The results indicated that the Bio-AEP, in which pancreatic endocrine cells were trapped in a mixed matrix and with a 0.1 microm pore size membrane, should be useful for xenoimplantation into diabetic animals and may open a new field in the therapy of human diabetics.

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“…Diffusion chambers have been widely used for some years as a means of maintaining the survival and function of the enclosed tissue after transplantation by allowing the passage of essential nutrients, whilst at the same time separating the implanted tissue from the cells of the host which would otherwise be harmful to the implant. This technique has been applied to a variety of tissues including thyroid [10], thymus [11], ovarian tissue [12] and haemopoietic cells [13]. Diffusion chambers constructed of Millipore filters have been used to enclose pancreatic islet implants in studies on hereditary obese mice [14,15], while very transient reduction in plasma glucose was demonstrated in diabetic rats xenografted with piscine islets [16] in Millipore envelopes.…”

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confidence: 99%

Problems in the use of polycarbonate diffusion chambers for syngeneic pancreatic islet transplantation in rats

Theodorou

Tyhurst

et al. 1980

Diabetologia

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Islets of Langerhans have been enclosed in polycarbonate diffusion chambers and transplanted intraperitoneally to syngeneic streptozotocin diabetic rats. Direct implantation of 1100--1400 islets in these chambers failed to reverse diabetes during a period of 12 weeks, and viable islet tissue was not recoverable at the end of this period. Islets placed in chambers which had been implanted 3--12 weeks previously similarly failed to lower blood glucose of diabetic recipients, as a result of lack of survival of the islets. Insulin infusion into chambers previously implanted in vivo, I125 insulin diffusion studies in chambers recovered 6--8 weeks after implantation, and scanning electron microscopy of the recovered membranes all indicated that the pores were not totally occluded. The failure of islet transplantation via chambers in this simple syngeneic model has discouraging implications for their use as a means of avoiding allograft rejection.

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An Improved Diffusion Chamber (DC) for Culture of Hematopoietic Cells

Cited by 10 publications

References 0 publications

Membrane immunoisolation of a diffusion chamber for a bioartificial pancreas

Membrane immunoisolation of a diffusion chamber for a bioartificial pancreas

Membrane Immunoisolation of a Diffusion Chamber for a Bioartificial Pancreas

Problems in the use of polycarbonate diffusion chambers for syngeneic pancreatic islet transplantation in rats

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