An improved cage system for the bioassay of<i>Metarhizium anisopliae</i>on<i>Thrips tabaci</i>(Thysanoptera: Thripidae)

Pourian, Hamid-Reza; Ezzati-Tabrizi, Reyhaneh; Talaei-Hassanloui, Reza

doi:10.1080/09583150802290618

Cited by 4 publications

(2 citation statements)

References 15 publications

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“…Conidial concentrations were calculated using a Neubauer hemocytometer. Like Pourian et al (2008), germination percentage was calculated by counting 100 conidia on each PDA plate following 20 hours of incubation and then 48 hours (for slow-germinated but viable conidia) at 25±2°C. The fresh conidial germination rate of > 85-90% on PDA was assessed after a 20 h incubation at 25±2°C.…”

Section: Bioassaysmentioning

confidence: 99%

Isolation of entomopathogenic fungi from cultivated and uncultivated soils and evaluation of virulence against Callosobruchus maculatus (F.) (Coleoptera:Chrysomelidae)

Mehrmoradi¹,

Jamali²,

Pourian³

2022

J. Ent. Soc. Iran

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We aimed to explore the entomopathogenic fungi (EPF) from the different ecosystems including forests, gardens, fields, and rangeland soils in Kermanshah province, Iran. Trials were carried out on morphological, molecular characterization, diversity indices, and virulence assessments of indigenous EPF from 41 sampling sites of various localities. Using the Ephestia kuehniella (Zeller) as host bait, 114 fungal isolates were recovered-i.e., 39 from forests, 38 from fields, 22 from rangelands, and 15 from garden soils. Based on morphological features and the sequence analysis of the internal transcribed spacer (ITS) of the ribosomal DNA, the recovered entomopathogenic fungi were identified as

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Section: Bioassaysmentioning

confidence: 99%

Isolation of entomopathogenic fungi from cultivated and uncultivated soils and evaluation of virulence against Callosobruchus maculatus (F.) (Coleoptera:Chrysomelidae)

Mehrmoradi¹,

Jamali²,

Pourian³

2022

J. Ent. Soc. Iran

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“…We used C 1 V 1 = C 2 V 2 formula for preparing of the latest concentration (1×10 7 conidia/ml) were prepared by serial dilution from 1×10 8 conidia/ml. For bioassay of Unauthenticated Download Date | 5/11/18 2:31 PM larvae with conidial formulations, the bioassay chamber consisted of common hyaline canisters for storage of 35 mm photography film, 5 cm hight and 3 cm in diameter (Pourian et al 2008). Briefly, we reduced the height to 3 cm by cutting off the top 2 cm and made a hole, 1 cm in diameter in the lid and covered it with polyester net in size of 200 µm meshes.…”

Section: Bioassaymentioning

confidence: 99%

Effect of Formulating of Beauveria Bassiana Conidia on their Viability and Pathogenicity to the Onion Thrips, Thrips Tabaci Lind. (Thysanoptera: Thripidae)

Ezzati-Tabrizi¹,

Talaei-Hassanloui²,

Pourian³

2009

Journal of Plant Protection Research

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Abstract:The wettable powder was prepared on the basis of aerial conidia of two isolates of the entomopathognic fungus, Beauveria bassiana. Viability and pathogenicity of conidia products were evaluated against the second-instar larva of Thrips tabaci in four cases;Conidial-product Maintained in Refrigerator (CMR), Conidial-product Maintained in Laboratory (CML), New Formulated Conidia (NFC) and New Conidia without formulation (NC). Analysis of corrected seven-day total mortality data revealed that there were significant differences among these product-cases in their pathogenicity to thrips larvae. Recorded mortality rates for CMR, CML, NFC and NC showed that the pathogenicity of CML was lower compared to three other cases for both isolates. In the next step, inorganic salts (MgCl 2 , NH 4 PO 4 , KH 2 PO 4 , MgSO 4 and NaCl) were added at a rate of 0.1 M into the both CMR and CML products. Bioassay results indicated that caused total mortality of thrips larvae increased with adding of salts. Our results showed that applied carriers and salts have positively effected preserving of conidia viability and pathogenicity to the second-instar larva of the onion thrips.

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Laboratory techniques used for entomopathogenic fungi

Inglis

Enkerli

Goettel

2012

Manual of Techniques in Invertebrate Pathology

173

130

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An improved cage system for the bioassay ofMetarhizium anisopliaeonThrips tabaci(Thysanoptera: Thripidae)

Cited by 4 publications

References 15 publications

Isolation of entomopathogenic fungi from cultivated and uncultivated soils and evaluation of virulence against Callosobruchus maculatus (F.) (Coleoptera:Chrysomelidae)

Isolation of entomopathogenic fungi from cultivated and uncultivated soils and evaluation of virulence against Callosobruchus maculatus (F.) (Coleoptera:Chrysomelidae)

Effect of Formulating of Beauveria Bassiana Conidia on their Viability and Pathogenicity to the Onion Thrips, Thrips Tabaci Lind. (Thysanoptera: Thripidae)

Laboratory techniques used for entomopathogenic fungi

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