2020
DOI: 10.1074/mcp.ra119.001857
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An Improved Boosting to Amplify Signal with Isobaric Labeling (iBASIL) Strategy for Precise Quantitative Single-cell Proteomics

Abstract: Mass spectrometry (MS)-based proteomics has great potential for overcoming the limitations of antibody-based immunoassays for antibody-independent, comprehensive, and quantitative proteomic analysis of single cells. Indeed, recent advances in nanoscale sample preparation have enabled effective processing of single cells. In particular, the concept of using boosting/carrier channels in isobaric labeling to increase the sensitivity in MS detection has also been increasingly used for quantitative proteomic analys… Show more

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Cited by 133 publications
(216 citation statements)
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“…6E). The abundance pattern of RRM2 is consistent with previous proteomic studies and targeted degradation of RRM2 in late G2/early mitosis by the cyclin F-SCF complex [5] [26]. Example proteins from this cluster include TPX2 and Aurora A kinase (Fig.…”
Section: High Temporal Resolution Analysis Of An Unperturbed Cell Cycsupporting
confidence: 88%
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“…6E). The abundance pattern of RRM2 is consistent with previous proteomic studies and targeted degradation of RRM2 in late G2/early mitosis by the cyclin F-SCF complex [5] [26]. Example proteins from this cluster include TPX2 and Aurora A kinase (Fig.…”
Section: High Temporal Resolution Analysis Of An Unperturbed Cell Cycsupporting
confidence: 88%
“…The advanced PRIMMUS method presented here significantly reduces the number of cells required, i.e. ~10 3 versus ~10 5 .…”
Section: An Improved Primmus For Proteomic Analysis Of Low Cell Numbementioning
confidence: 99%
See 1 more Smart Citation
“…6a. This effect of an increased MS2 AGC target is consistent with previous observations [23], and previously used analytical parameters for SCoPE-MS and SCoPE2 analysis have corresponding to the high AGC target regime [10,17]. In this regime, the isobaric carrier does not limit MS2 accumulation times .…”
Section: Effects Of Isobaric Carriers On Peptide Identificationsupporting
confidence: 91%
“…The field has advanced rapidly since those initial reports. More than 1000 protein groups can now be reliably profiled from single cells using both label-free (13), and isobaric labeling workflows (14)(15)(16), and ~6000 protein groups can be profiled from samples comprising just a few hundred cells (17). Thus, a tradeoff still exists between sample size and proteome coverage, but it has dramatically shifted in favor of much smaller samples.…”
mentioning
confidence: 99%