An improved assay of corticosterone in rat serum and adrenal tissue

Givner, Morris L.; Rochefort, J. G.

doi:10.1016/0039-128x(65)90061-9

Cited by 64 publications

(10 citation statements)

References 8 publications

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“…This is similar to the value of 3.0 ± 1.0 fig/lOO mg of adrenal tissue (mean ± SD) which was determined by UV spectro photometry [1]. Because the mean weight of the adrenal glands was 20 mg, this concentration could also be expressed as 0.312 ±0.127 fig per adrenal gland (m ean± SD); this compares well with the value of 0.5 fig corticosterone per adult rat adrenal determined by fluores cent spectrophotometry [6]. Unfortunately there arc no published determinations of cortisol, deoxycortisol or deoxycorticosterone.…”

Section: Resultssupporting

confidence: 57%

“…The concentration of corticosterone in adult Sprague-Dawley rat adrenal tissue determined by the CBG method (mean± SD = 1.56±0.64 ,wg/100 mg or 0.312 ± 0.127 fig per adrenal gland) favorably compares with the less specific values in Wistar rats determined by either the colorimetric tctrazole blue method (m ean± SD = 3.4d: 1 fig/iOO mg) [1] or UV spectro photometry (3 1 fig/100 mg [1] and in adult white rats of unspecified strain measured by UV spectrophotometry (0 .5 fig per gland) [6]. The fact that the value determined by this study is lower than the previous values could be due to species variation, coincidental varia tions in metabolic states, nonsteroidal fluorescence in the UV method or a-ketol contamination in the tetrazole blue method.…”

Section: Discussionmentioning

confidence: 65%

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Fetal Rat Adrenal Steroidogenesis

Kalavsky¹

1971

Neonatology

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Section: Resultssupporting

confidence: 57%

Section: Discussionmentioning

confidence: 65%

Fetal Rat Adrenal Steroidogenesis

Kalavsky¹

1971

Neonatology

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“…The similarity of corticosterone values between steroid-treated (1.0 mg) and control animals at 15 min fol lowing ether stress seen in the present studies differs from data previously obtained in this laboratory [29] showing significant suppres sion of plasma corticosterone at 15 min as well as at later time points. We feel that the use of a micro-rather than a macro-assay [5] for plasma corticosterone in the present stud ies provides more reliable values and that this methodological difference may contribute to this discrepancy.…”

Section: Discussionmentioning

confidence: 80%

Effects of Neonatal Hydrocortisone Treatment on Pituitary and Adrenocortical Response to Stress in Young Rats

Erskine¹,

Geller²,

Yuwiler³

1979

Neuroendocrinology

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Administration of corticosteroids to rat pups within the first several days of life results in retardation of several behavioral, neurophysiological, and biochemical developmental patterns. At 25 days of age, animals treated neonatally with 1.0, 0.5, or 0.1 mg hydrocortisone acetate showed dose-dependent decreases in plasma ACTH following 2.5 min continuous exposure to ether. On day 20, plasma corticosterone values did not differ between these groups 15 min after ether stress, but lower values were seen 60 min after ether in animals treated at birth with 0.5 mg hydrocortisone. At 45-48 days of age, hydrocortisone-treated animals exposed to one of 2 different stressors showed decreased plasma corticosterone response to stress; females had lower corticosterone levels following both stressors, while males showed suppressed corticosterone levels following exposure to the mild (novelty) but not to the intense (ether) stress. These data demonstrate that neonatal exposure to hydrocortisone results in decreased CNS-pituitary responsivity to stress at 20–25 days of age, and that the adrenocortical response to stress is impaired at 45–48 days of age.

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“…Any samples which required over 2 min to collect were not included in the data analysis. The blood samples were centrifuged, and the plasma was separated and frozen until assayed fluorometrically for corticosterone content by the method of Givner and Rochefort [7]. Corticosterone levels for each group were analyzed statistically using the Student's t test and the two-factor analysis of variance [18], Not all control animals tested on trial 1 were sampled on trial 18: 13 control animals were tested and terminated on trial 10 in order to determine whether the fourth trial of the day might influence the adrenal response to the novel stress.…”

Section: Methodsmentioning

confidence: 99%

Adrenocortical Response to Novelty Stress in Rats with Dentate Gyrus Lesions

Johnson¹,

Moberg²

1980

Neuroendocrinology

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Male rats exposed to a novel environment exhibited a marked rise of plasma corticosterone in response to the initial exposure. These animals showed a significant but not complete habituation of their adrenal response after 18 exposures. Following their first exposure to a novel environment, animals with bilateral lesions in the dentate gyrus of the hippocampus had plasma corticosterone concentrations which were significantly lower than those observed for the control animals. Whereas the control groups demonstrated a significant decrease in their adrenal response following 18 exposures (habituation), there was no decrease of the adrenocortical response to novelty stress within the dentate-lesioned group between trials 1 and 18. The effect of the dentate lesions appeared to be specific to the behavioral stress: dentate lesions failed to alter resting levels, or the animal’s adrenal responses to laparotomy stress and ether inhalation.

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An improved assay of corticosterone in rat serum and adrenal tissue

Cited by 64 publications

References 8 publications

Fetal Rat Adrenal Steroidogenesis

Fetal Rat Adrenal Steroidogenesis

Effects of Neonatal Hydrocortisone Treatment on Pituitary and Adrenocortical Response to Stress in Young Rats

Adrenocortical Response to Novelty Stress in Rats with Dentate Gyrus Lesions

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