An improved and reliable chili pepper ( Capsicum annuum L.) plant regeneration method

Ramírez-Malagón, Rafael; Ochoa-Alejo, Neftalí

doi:10.1007/s002990050212

Cited by 7 publications

(8 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…There have been reports that, in some species, the TDZ can stimulate both processes, somatic embryogenesis and the proliferation of adventitious shoots (Bates et al, 1992;Fiola et al, 1990;Santana-Buzzy et al, 2005), and although there are few reports available, TDZ has been used in the range of 0.5 to 10 mM to stimulate somatic embryogenesis from cotyledons of white ash (Bates et al, 1992;Preece and Bates, 1990;), eastern black walnut (Neuman et al,1988(Neuman et al, , 1993, Rubus (Fiola et al,1990), and Vitis vinifera L. (Matsuta and Hirabayashi, 1989). TDZ was successfully applied to induce organogenesis and somatic embryogenesis from different explants such as half seedlings, intact seedlings, immature embryos, embryonic hypocotyls, embryonic cotyledons, cotyledons, leaves, cotyledonary nodes, and shoot tips of pepper (Binzel et al, 1996a(Binzel et al, , 1996bDabauza and Pena, 2001;Hyde and Phillips, 1996;Kaparakis and Alderson, 2002;Manoharan et al, 1998;Ramírez-Malagon and Ochoa-Alejo, 1996;Szasz et al, 1995;Venkataiah et al, 2003). Thidiazuron shows high efficiency in stimulating cytokinindependent shoot regeneration in a wide variety of plants (Huetteman and Preece, 1993;Murthy et al, 1998) and, in particular, for the micropropagation of many recalcitrant species (Huetteman and Preece, 1993).…”

Section: Discussionmentioning

confidence: 99%

Somatic Embryogenesis in Habanero Pepper (C. chinense Jacq.) From Cell Suspensions

Zapata-Castillo¹,

Flick²,

López-Puc³

et al. 2007

horts

View full text Add to dashboard Cite

To induce the somatic embryogenesis of Habanero pepper, different culture media and different types of explants (node, internode, hypocotyl, half seeds, and fruit segments) were evaluated. For the induction of embryogenic callus, 9.05 μm of 2,4-dichlorofenoxiacetic acid, 3% sucrose, and 0.8% gelrite were added to the basic MS medium over a period of 30 days at 25 ± 2 °C under continuous light (40–50 μmol·m2·s−1). Once the callus formed, they were transferred to liquid medium using the same induction formulation. Somatic embryogenesis only occurred from explants of hypocotyl and in the presence of 3.4 μm thidiazuron. This constitutes the first proposal of a protocol for the “induction of somatic embryogenesis in Habanero pepper (Capsicum chinense Jacq.) from cell suspensions.”

show abstract

Section: Discussionmentioning

confidence: 99%

Somatic Embryogenesis in Habanero Pepper (C. chinense Jacq.) From Cell Suspensions

Zapata-Castillo¹,

Flick²,

López-Puc³

et al. 2007

horts

View full text Add to dashboard Cite

show abstract

“…Additionally, radical differences in the regeneration response have been reported at the intervarietal level and explant source. Therefore, specific cultivar and tissue culture media have been devised to optimize regeneration from specific cultivars (Christopher and Rajam, 1994;Ramírez-Malagón and Ochoa-Alejo, 1996;Venkataiah and Subhash, 2001). Thus, the strong influence of the pepper variety (genotype) makes it necessary to optimize regeneration protocols for specific cultivars.…”

mentioning

confidence: 99%

In Vitro Germplasm Conservation of Habanero Pepper (Capsicum chinense Jacq.)

Montalvo-Peniche

Iglesias-Andreu

Mijangos‐Cortés

et al. 2007

horts

View full text Add to dashboard Cite

To determine the effect of different nitrogen sources and osmotic regulators on minimal growth of Habanero pepper (Capsicum chinense Jacq.) germplasm for in vitro conservation, different concentrations of nitrate, sucrose, mannitol, and sorbitol were evaluated. The micropropagation system based on Santana-Buzzy et al. (2006) culture medium was modified in its nitrate concentrations: reduced to 50% and increased to 150%, and osmoregulators were added to the basal culture media: sucrose (6% and 8%), mannitol (2%, 4%, and 8%), or sorbitol (2%, 4%, and 8%). The apical meristems of germinated plants were cultivated in the different treatments for 35 weeks without subculture. Results have demonstrated that mannitol at 2% had the better effect on minimal growth of the plantlets and did not affect the plant physiology and quality. The plantlets remained small in size, turgent, with green leaves and stems and looked like normal plants until to the end of the evaluation period. Changes in nitrogen media concentration did not prove to be adequate for conserving because they affected the plantlet quality (they became chlorotic). The presence of sorbitol and high osmolite concentrations induced minimal growth but reduced the plant quality. Sucrose at mid or low concentrations did not induce minimal growth.

show abstract

“…Data were analyzed using the General Linear Models Procedure (SAS Institute, Cary, N.C.). enced by photoperiod during germination and growth (Ramirez-Malagon and Ochoa-Alejo, 1996), seedling age at excision (Ramirez-Malagon and Ochoa-Alejo, 1996;Yang et al, 1991), and BA concentration (Calvo and Sequra, 1988;Kameya and Widholm, 1981;Yang et al, 1991). In A. majus, the effects of different germination and growth photoperiods, hypocotyl ages at excision, and BA concentrations in the culture medium have been evaluated for adventitious shoot formation, but studies to optimize shoot formation are lacking.…”

mentioning

confidence: 99%

Adventitious Shoot Formation on Excised Hypocotyls of Antirrhinum majus L. (Snapdragon) In Vitro

Schroeder¹,

Stimart²

1999

HortSci

View full text Add to dashboard Cite

Hypocotyls from Antirrhinum majus L. were excised at 2 weeks of age from seedlings grown under a 16-hour photoperiod or continuous darkness. Explants were cultured on modified Murashige-Skoog (MS) medium containing 0, 0.44, 2.22, 4.44, 8.88, or 44.4 μm BA to investigate adventitious shoot formation. Excised hypocotyls from eight commercial cultivars, three inbred lines, and an F1 hybrid between two of the inbreds were cultured on MS medium containing 2.22 μm BA to assess genotypic effects on adventitious shoot formation. The influence of seedling age was assessed by excising hypocotyls from seedlings at 6, 10, 14, 18, 22, 26, or 30 days. Optimal conditions for adventitious shoot formation on excised hypocotyls included: seedling growth in a lighted environment, use of hypocotyls from 10-day-old seedlings, and culture on medium containing 2.22 μm BA for 3 weeks. Under these conditions, up to a 5-fold improvement in number of shoots per hypocotyl over previous studies was achieved. Adventitious shoot formation was genotype-dependent and appeared to be a dominant trait. Chemical name used: N6-benzyladenine (BA).

show abstract

An improved and reliable chili pepper ( Capsicum annuum L.) plant regeneration method

Cited by 7 publications

References 0 publications

Somatic Embryogenesis in Habanero Pepper (C. chinense Jacq.) From Cell Suspensions

Somatic Embryogenesis in Habanero Pepper (C. chinense Jacq.) From Cell Suspensions

In Vitro Germplasm Conservation of Habanero Pepper (Capsicum chinense Jacq.)

Adventitious Shoot Formation on Excised Hypocotyls of Antirrhinum majus L. (Snapdragon) In Vitro

Contact Info

Product

Resources

About