An improved amperometric determination of xanthine with xanthine oxidase nanoparticles for testing of fish meat freshness

Joon, Archana; Ahlawat, Jyoti; Aggarwal, Vishakha; Jaiwal, Ranjana; Pundir, C.S.

doi:10.1016/j.sbsr.2021.100437

Cited by 10 publications

(1 citation statement)

References 30 publications

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“…For the samples aged 2, 4, and 7 days, we measured increasing XA concentrations of 10 μM (±0.86 μM), 17 μM (±0.72 μM), and 32 μM (±1.1 μM), respectively. This increasing XA concentration results from purine nucleotide breakdown during the degradation of fish meat, as seen in previous studies. ,,− …”

Section: Resultssupporting

confidence: 66%

Amperometric Determination of Xanthine Using Nanostructured NiO Electrodes Loaded with Xanthine Oxidase

Tripathi

Elias

Jemere

et al. 2022

ACS Food Sci. Technol.

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Nickel oxide (NiO) thin films prepared by glancing angle deposition (GLAD) were investigated as electrodes for enzymatic electrochemical quantification of xanthine, a noted indicator of meat freshness. The large surface area of the macroporous GLAD NiO electrodes provided a suitable scaffold to successfully immobilize the enzyme xanthine oxidase (XO), and this XO immobilization was characterized by cyclic voltammetry, electrochemical impedance spectroscopy, and X-ray photoelectron spectroscopy. The XO-modified GLAD NiO electrodes electrochemically oxidize xanthine, with electron transfer from this adsorbed XO to the (Ni2+/Ni3+) redox species resulting in a strong amperometric response to xanthine in a reagent-free alkaline medium. Under optimal conditions, the fabricated xanthine biosensor exhibited a rapid response (∼7 s), wide dynamic range (0.1–650 μM), good reproducibility (relative standard deviation of ∼4%, n = 18), superior limit of detection (37 nM), and very high sensitivity (1.1 μA·μM–1·cm–2 in the low concentration range from 0.1–5 μM and 0.3 μA·μM–1·cm–2 in the higher concentration range from 5–650 μM). The biosensor was also evaluated against a selection of potential interferents commonly found in fish samples (hypoxanthine, uric acid, glucose, and sodium benzoate), demonstrating good selectivity toward xanthine. A low Michaelis–Menten constant (K m) of 0.4 mM for xanthine signifies the high affinity of the enzymatic sensor toward the target analyte. Measurements in real fish samples were also successfully performed, revealing strongly increased xanthine sensitivity in the presence of fish matrices (from 0.085 μA μM–1 without fish extract to as much as 0.27 μA μM–1).

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Section: Resultssupporting

confidence: 66%

Amperometric Determination of Xanthine Using Nanostructured NiO Electrodes Loaded with Xanthine Oxidase

Tripathi

Elias

Jemere

et al. 2022

ACS Food Sci. Technol.

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A photoelectrochemical sensor combining CS−GSH−CuNCs and xanthine oxidase for the detection of xanthine

Chen

Luo

et al. 2022

ChemElectroChem

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A photoelectrochemical (PEC) sensor combining chitosan‐coated and glutathione‐protected copper nanocluster (CS−GSH−CuNCs) with peroxidase‐like activity and xanthine oxidase (XAO) was developed for rapid detection of xanthine. Firstly, CS−GSH−CuNCs was prepared by glutathione (GSH) reduction using chitosan (CS) as protective agent, and then was modified on titanium dioxide nanoparticles (TiO2 NPs)/ITO electrode which was prepared by sintering titanium dioxide (TiO2) on the ITO electrode surface by high temperature calcination. After excited by light at 350 nm, the photoinduced electrons from the lowest unoccupied molecular orbital (LUMO) of CS−GSH−CuNCs were transferred to the conduction band of TiO2, which resulted in spatial separation of electron‐hole and enhancement of photocurrent signal. XAO oxidizes xanthine to produce hydrogen peroxide (H2O2). Part of the photoinduced electrons from CS−GSH−CuNCs transferred and catalyzed the reduction of H2O2, resulting in the reduction of the photocurrent. Under optimal conditions, the PEC sensor exhibits good sensitivity and reproducibility with the limit of detection (LOD) of 6.6 nmol ⋅ L−1 and a relative standard deviation of 3.5 % for 10 replicate detections of 1.00 μmol ⋅ L−1 xanthine and the linear range of 0.04–90.0 μmol ⋅ L−1 for xanthine. Furthermore, the PEC sensor presents nice selectivity owing to its enzyme‐like activity and was successfully applied to human urine, indicating its great potential for real sample analysis.

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Electrochemical xanthine biosensor based on carbon nanofiber and ferrocene carboxylic acid for the assessment of fish freshness

Can,

Kaçar Selvi,

Canel

et al. 2024

Ionics

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An improved amperometric determination of xanthine with xanthine oxidase nanoparticles for testing of fish meat freshness

Cited by 10 publications

References 30 publications

Amperometric Determination of Xanthine Using Nanostructured NiO Electrodes Loaded with Xanthine Oxidase

Amperometric Determination of Xanthine Using Nanostructured NiO Electrodes Loaded with Xanthine Oxidase

A photoelectrochemical sensor combining CS−GSH−CuNCs and xanthine oxidase for the detection of xanthine

Electrochemical xanthine biosensor based on carbon nanofiber and ferrocene carboxylic acid for the assessment of fish freshness

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