An improved agar plate method for rapid assessment of chemical inhibition to microbial populations

Synthesis and antibacterial activity of cefoxitin metal complexes

“…In vitro antibacterial activities of cefoxitin and its complexes were tested using the paper disc diffusion method [12]. The chosen strains were G(+) Staphylococcus aureus ATCC 25923 and Enterococcus faecalis W.H.O.…”

Concentrations of Copper Thought To Be Toxic to Escherichia coli Can Induce the Viable but Nonculturable Condition

“…It has been suggested that toxicity occurs due to membranebound copper catalyzing the formation of hydroperoxide free radicals (16). Toxicity levels are determined by examining bacterial growth in the presence of various concentrations of an agent on solid or liquid growth medium (5,9,13). A lack of growth is considered to indicate that the agent has killed cells.…”

“…These results indicate that copper can induce E. coli to enter the VBNC condition. The concentrations of copper used in this study include those considered toxic (9,13) and are higher than those used to examine copper-induced cell injury (6,17). That high concentrations of copper do not kill all cells suggests that current growth-based microbiological methods for assaying toxicity result in an undercount of the number of viable cells through incorrect scoring of VBNC cells as dead.…”

Synthesis and antibacterial activity of copper(II) complexes with sulphathiazole and cephalosporin ligands

“…Antibacterial activities were tested using the paper disc diffusion method [18]. The chosen strains were G(+) Staphylococcus aureus ATCC 25923 and G(-) Escherichia coli ATCC 11775, Pseudomonas aeruginosa ATCC 27853, Klebsiella pneumoniae ATCC 23357, Salmonella enteritidis CDC 64 and Bacillus subtilis ATCC 6051.…”