An improved acetic acid‐urea polyacrylamide electrophoresis method to evaluate bovine sperm protamines

Hamilton, Thais Rose dos Santos; Simões, Rafael Plana; Assumpção, Mayra Elena Ortiz D’Ávila

doi:10.1111/rda.13941

Cited by 2 publications

(1 citation statement)

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“…Nevertheless, the relationship between protamination and fertility in bulls is a new topic, being at first described by relative values and recently through absolute quantifications (Hamilton et al, 2019). As protamines are basic proteins, they also can be measured by a modified western blot because protamines are negative-charged proteins, being been already validated for bulls (Hamilton et al, 2021).…”

Section: Protamination Check-upmentioning

confidence: 99%

What is known so far about bull sperm protamination: a review

2022

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Sperm routinary fitness evaluation is not sufficient to predict bull reproductive capacity as they present differences in fertility up to 40%. Among the defects which compromise spermatozoa functionality, new approaches consider the study of sperm chromatin, which is the core structure containing paternal genetic information. Sperm chromatin needs to be compacted to maintain the integrity of DNA, which occurs by binding nucleoproteins with high affinity to DNA. In the last stages of sperm maturation, chromatin is hyper-compacted by basic proteins called protamines in a process named protamination. In this review, we summarized intrinsic and extrinsic factors that are suggested to influence protamination in bull spermatozoa, considering old and new evidence from human and murine spermatozoa. Also, the current approaches to evaluate bull protamination and its relationship with fertility were described. Nevertheless, the physiological mechanisms of protamination are still poorly understood.

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Section: Protamination Check-upmentioning

confidence: 99%

What is known so far about bull sperm protamination: a review

2022

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Male Infertility Mediated by Gene Mutations

Guo

2024

Male-Mediated Developmental Toxicity

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Impairment of spermatogenesis can result from cell-cycle arrest or death of germ cells and potentially occur at any stage of life. Testis maldescensus, fluctuations of hormonal support and a wide range of exogenous factors may contribute to this impairment, likely resulting in a significant reduction in the number of sperm in the ejaculate (oligozoospermia) or azoospermia (the absence of sperm from semen). The testis pathological processes that result in the ablation of a particular cell type will distort the apparent relative expression levels of genes expressed in the testis, given that different cell types have different profiles of gene expression. Known genetic causes of male infertility include chromosome diseases, mitochondrial DNA (mtDNA) mutation, single-gene disease, and multifactor disease. The number of genetic abnormalities found in semen and etiological categories is increasing. Genetic research has made great progress in elucidating the causes of male infertility. Based on the support of high-throughput sequencing technology, more than 2000 genes were found to have mutations or abnormal expression related to spermatogenesis. This has made great contributions to diagnostic value, clinical decision-making, and appropriate genetic counselling. Therefore, this chapter will focus on the influence of gene mutations on male infertility from the perspective of genetics.

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An improved acetic acid‐urea polyacrylamide electrophoresis method to evaluate bovine sperm protamines

Cited by 2 publications

References 25 publications

What is known so far about bull sperm protamination: a review

What is known so far about bull sperm protamination: a review

Male Infertility Mediated by Gene Mutations

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