An improved Abbott ARCHITECT® assay for the detection of hepatitis B virus surface antigen (HBsAg)

Novel HBsAg markers tightly correlate with occult HBV infection and strongly affect HBsAg detection

“…A new Abbott ARCHITECT Ò assay for the detection of HBsAg has been recently proposed to specifically enhance the detection of some HBsAg mutants at position 122 and 123 (Lou et al, 2011). The impact of the other genetic markers we identified on the performance of this new assay has not yet been defined.…”

Amino Acid Substitutions at Positions 122 and 145 of Hepatitis B Virus Surface Antigen (HBsAg) Determine the Antigenicity and Immunogenicity of HBsAg and InfluenceIn VivoHBsAg Clearance

“…Some clinical samples may contain vtHBsAgs at lower levels and therefore will not be recognized in HBsAg immunoassays. In addition, the failure of the detection of some HBsAg mutants can be found in recent studies (17). To solve this issue, the development of new kits with MAb pools or polyclonal Abs are urgently needed.…”

Variable capacity of 13 hepatitis B virus surface antigen assays for the detection of HBsAg mutants in blood samples

“…Since vaccination coverage 27 and long-term anti-viral therapy of chronic carriers 28,29 that could induce mutations in the polymerase gene overlapping with S-gene, 30 may contribute to increasing the prevalence of HBsAg variants and despite progresses to improve the capacity of HBsAg assays to detect mutants, 31 surveillance programs of HBV genetic variability are needed. The limitations of some immunoassays in detecting HBV variants should encourage the health authorities to implement an evaluation protocol that systematically includes HBsAg variant panels.…”