An important role of the heat shock response in infected cells for replication of baculoviruses

Lyupina, Yu. V.; Dmitrieva, S. B.; Timokhova, Anna V.; Beljelarskaya, Svetlana N.; Zatsepina, Olga G.; Evgen’ev, Michael B.; Mikhailov, Victor S.

doi:10.1016/j.virol.2010.07.039

Cited by 41 publications

(39 citation statements)

References 30 publications

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“…The induction of heat shock proteins (HSPs) of the 70-kDa family (HSP/HSC70) in Sf-9 cells after infection with AcMNPV was monitored by Western blot analysis (Lyupina et al,2010). were selected through stringent procedure after considering their economic value to the sericulture industry.…”

Section: Discussionmentioning

confidence: 99%

Differential Level of Host Gene Expression Associated with Nucleopolyhedrovirus Infection in Silkworm Races of Bombyx mori

Lekha¹,

Vijayagowri²,

Sasibhushan³

et al. 2014

International Journal of Industrial Entomology

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The variation in the level of immune response related gene expression in silkworm, Bombyx mori following infection with Bombyx mori nucleopolyhedrovirus (BmNPV) was analyzed at different time intervals. The occlusion bodies of BmNPV orally inoculated to the two most divergent silkworm races viz., Sarupat (resistant to BmNPV infection) and CSR2 (susceptible to BmNPV infection) were subjected to oral BmNPV inoculation. The expression profile of gp41 gene of BmNPV in the Sarupat and CSR2 races revealed that the virus could invade the midguts of both susceptible and resistant races. However, its multiplication was significantly less in the midgut of resistant race, while, in the susceptible race, the viral multiplication reached maximum level within 12 h. These findings indicate that potential host genes are involved in the inhibition of viral multiplication within larval midgut.

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Section: Discussionmentioning

confidence: 99%

Differential Level of Host Gene Expression Associated with Nucleopolyhedrovirus Infection in Silkworm Races of Bombyx mori

Lekha¹,

Vijayagowri²,

Sasibhushan³

et al. 2014

International Journal of Industrial Entomology

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“…Based on our findings regarding the IE2 and HSP relationship in a mammalian system, we hypothesized that HSPs stimulate IE2 protein stabilization. To verify this hypothesis, we examined IE2 protein levels upon treatment with a heat shock response inhibitor, KNK437, which has also previously been applied in an insect cell system [23]. As shown in Fig 9A, upon baculovirus infection of Sf cells, IE2 protein was detectable as early as 2 hpi, followed by a decrease after 4 and hsc70 (A2) genes were quantified by RT-qPCR using specific primers.…”

Section: Hsps Stabilize the Ie2 Protein During Baculovirus Infectionmentioning

confidence: 94%

“…Although a previous study has shown that inhibition of the heat shock response decreased the levels of viral replication in infected cells [23], the viral factor(s) that are responsible for this show that at 48 hpt, IE2 formed mature nuclear bodies that included a high concentration of G-actin (green) and enlarged, activated Pol II dots (red). KNK437 treatment alone (panels e to h) led to a failure in IE2 nuclear body formation as well as G-actin and activated Pol II recruitment, whereas for KNK437 in combination with MG132 (panels i to l), mature IE2 nuclear bodies could again be observed.…”

Section: The Interplay Between Hsc70 and Ie2 During Baculovirus Infecmentioning

confidence: 94%

“…First, a benzylidene lactam compound, KNK437-the most widely-used heat shock response inhibitor [23,[36][37][38]]-was applied to our experiments. KNK437 prevents the development of thermotolerance by inhibiting the up-regulation of various HSPs at the transcriptional level [39].…”

Section: Ie2-expressing Recombinant Virus Transduction Stimulates Hspmentioning

confidence: 99%

“…We found that heat shock proteins (HSPs) are one of the major components that complex with the IE2 protein. Stimulation of a heat shock response during baculovirus infection and its importance for virus amplification has been frequently mentioned in the literature [23][24][25][26]. However, the viral factors that are responsible for this effect and the mechanism underlying the interaction between viral factors and HSPs are still unclear [27].…”

Section: Introductionmentioning

confidence: 99%

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Baculovirus IE2 stimulates the expression of heat shock proteins in insect and mammalian cells to facilitate its proper functioning

Chao¹

2016

2016 International Congress of Entomology

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Baculoviruses have gained popularity as pest control agents and for protein production in insect systems. These viruses are also becoming popular for gene expression, tissue engineering and gene therapy in mammalian systems. Baculovirus infection triggers a heat shock response, and this response is crucial for its successful infection of host insect cells. However, the viral protein(s) or factor(s) that trigger this response are not yet clear. Previously, we revealed that IE2-an early gene product of the baculovirus-could form unique nuclear bodies for the strong trans-activation of various promoters in mammalian cells. Here, we purified IE2 nuclear bodies from Vero E6 cells and investigated the associated proteins by using mass spectrometry. Heat shock proteins (HSPs) were found to be one of the major IE2-associated proteins. Our experiments show that HSPs are greatly induced by IE2 and are crucial for the trans-activation function of IE2. Interestingly, blocking both heat shock protein expression and the proteasome pathway preserved the IE2 protein and its nuclear body structure, and revived its function. These observations reveal that HSPs do not function directly to assist the formation of the nuclear body structure, but may rather protect IE2 from proteasome degradation. Aside from functional studies in mammalian cells, we also show that HSPs were stimulated and required to determine IE2 protein levels, in insect cells infected with baculovirus. Upon inhibiting the expression of heat shock proteins, baculovirus IE2 was substantially suppressed, resulting in a significantly suppressed viral titer. Thus, we demonstrate a unique feature in that IE2 can function in both insect and nonhost mammalian cells to stimulate HSPs, which may be associated with IE2 stabilization and lead to the protection of the its strong gene activation function in mammalian cells. On the other hand, during viral infection in insect cells, IE2 could also strongly stimulate HSPs and ultimately affect viral replication.

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Transcriptomic analysis of interactions between Hyphantria cunea larvae and nucleopolyhedrovirus

Sun

Liu

Sun

et al. 2018

Pest Management Science

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An important role of the heat shock response in infected cells for replication of baculoviruses

Cited by 41 publications

References 30 publications

Differential Level of Host Gene Expression Associated with Nucleopolyhedrovirus Infection in Silkworm Races of Bombyx mori

Differential Level of Host Gene Expression Associated with Nucleopolyhedrovirus Infection in Silkworm Races of Bombyx mori

Baculovirus IE2 stimulates the expression of heat shock proteins in insect and mammalian cells to facilitate its proper functioning

Transcriptomic analysis of interactions between Hyphantria cunea larvae and nucleopolyhedrovirus

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