“…Indeed, different sets of genes are transcriptionally impacted by tEVs when endothelial cells are cultured in static or low flow (Fig 4A, right). Among the genes upregulated by tEVs in flow‐stimulated endothelial cells, we found several pro‐angiogenic transcription factors, such as ID1, ID2, ID3, Hey1, Hey2, MAFB, Runx1 and HES1 (Benezra et al , 2001; Fischer et al , 2004; Kitagawa et al , 2013; Morioka et al , 2014). We further identified genes that are involved in two pro‐angiogenic signaling pathways, the Notch (HEY1, HEY2, HES1, JAG1) and the TGFß (Smad6, Smad7, Bambi, PMEPA1, Nog) pathways (Fig 4A, right).…”