An Important Role for RPRD1B in the Heat Shock Response

Abstract

During the heat shock response (HSR), heat shock factor (HSF1 in mammals) binds to target gene promoters, resulting in increased expression of heat shock proteins that help maintain protein homeostasis and ensure cell survival. Besides HSF1, only a relatively few transcription factors with a specific role in ensuring correctly regulated gene expression during the HSR have been described.

Transcriptional Stress Induces the Generation of DoGs in Cancer Cells

“…In general, any insult that generates stress in a cell causes the inactivation and overexpression of genes. A characteristic observed under oxidative stress, heat shock, and viral infections is the generation of DoGs [ 18 , 19 , 20 , 21 , 22 , 23 , 31 ]. We recently described how stress due to inhibition of the initiation of transcription mediated by RNA pol II causes the overexpression of some genes [ 16 ].…”

“…A characteristic that has recently been noted in cellular responses to different types of stress, such as osmotic stress and heat shock, as well as in some tumor cells and during viral infections, is the production of long noncoding RNAs (lncRNAs) generated from a readthrough transcription of genes transcribed by RNA pol II; in other words, these transcripts are not processed at the site where these events normally occur downstream of the polyadenylation site (PAS) [ 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 ]. These lncRNAs have been called downstream-of-gene (DoG)-containing transcripts.…”

Transcriptional stress induces the generation of DoGs in cancer cells

“…A characteristic that has recently been noted in cellular responses to different types of stress, such as osmotic stress and heat shock, as well as in some tumor cells and during viral infections, is the production of long noncoding RNAs (lncRNAs) generated from readthrough transcription of genes transcribed by RNA pol II; in other words, these transcripts are not processed at the site where these events normally occur downstream of the polyadenylation site (PAS) [Cardiello et al, 2018; Cugusi et al, 2022; Heinz et al, 2018; Morgan et al, 2022; Nemeroff et al, 1998; Rosa-Mercado et al, 2021; Shalgi et al, 2014; Vilborg et al, 2015]. These lncRNAs have been called downstream-of-gene (DoG)-containing transcripts.…”