An impedimetric immunosensor for the selective detection of CD34+ T-cells in human serum

Díaz-Fernández, Ana; Bernalte, Elena; Fernández-Ramos, Carlos; Moise, Sandhya; Estrela, Pedro; Lorenzo, Mirella Di

doi:10.1016/j.snb.2021.131306

Cited by 8 publications

(5 citation statements)

References 21 publications

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“…Capacitive biosensors have been used for the detection of a wide range of analytes (Table 1 ). This transduction approach is generalizable and compatible with the use of different molecular recognition elements: immunoreagents, both antigens for the recognition of antibodies [ 12 , 13 ] and antibodies for the recognition of proteins [ 14 ] or whole cells [ 15 ]; synthetic oligonucleotides for detecting RNA targets on the basis of the hybridization reaction [ 16 ] and aptamers for monitoring the corresponding targets after the affinity interaction [ 17 , 18 ]. Synthetic peptides or affimers [ 19 ] and macrocyclic compounds such as β-cyclodextrins [ 20 ] have also been employed in the design of the selective layer.…”

Section: Benefits Of Capacitive Sensors For Wearable Devicesmentioning

confidence: 99%

“…This strategy has been demonstrated for the detection of CD34 + -T-cells in 2% serum, using gold electrodes modified with a monolayer of mercaptoundecanoic acid and mercaptohexanol for anchoring the specific anti-CD34 + antibody. The sensor has a LOD of 44 cell mL −1 , a working range between 50 and 10 5 cell mL −1 and excellent selectivity to other cell types [ 15 ]. In addition, it is possible to construct the sensor on a flexible substrate more suitable for wearable devices using a thin-film gold sensor assembly with multiple channels.…”

Section: Benefits Of Capacitive Sensors For Wearable Devicesmentioning

confidence: 99%

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Capacitive spectroscopy as transduction mechanism for wearable biosensors: opportunities and challenges

Díaz-Fernández,

de-los-Santos-Álvarez,

Lobo-Castañón

2023

Anal Bioanal Chem

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Wearable sensors would revolutionize healthcare and personalized medicine by providing individuals with continuous and real-time data about their bodies and environments. Their integration into everyday life has the potential to enhance well-being, improve healthcare outcomes, and offer new opportunities for research. Capacitive sensors technology has great potential to enrich wearable devices, extending their use to more accurate physiological indicators. On the basis of capacitive sensors developed so far to monitor physical parameters, and taking into account the advances in capacitive biosensors, this work discusses the benefits of this type of transduction to design wearables for the monitoring of biomolecules. Moreover, it provides insights into the challenges that must be overcome to take advantage of capacitive transduction in wearable sensors for health. Graphical abstract

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Section: Benefits Of Capacitive Sensors For Wearable Devicesmentioning

confidence: 99%

Section: Benefits Of Capacitive Sensors For Wearable Devicesmentioning

confidence: 99%

Capacitive spectroscopy as transduction mechanism for wearable biosensors: opportunities and challenges

Díaz-Fernández,

de-los-Santos-Álvarez,

Lobo-Castañón

2023

Anal Bioanal Chem

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“…Carboxylic acids introduced via SAM formation, followed by peptide coupling 50-1 × 10 5 cells/mL 44 cells/mL in diluted serum [69] Bacteria answers to antibiotics / Tantalum silicide Polyethyleneimine layer for bacteria adsorption (non-specific) N/D N/D [57] Abbreviations: N/D: not detailed. TCID 50 : tissue culture infective dose.…”

Section: Peripheral Blood Mononuclear Cell Immunosensor Goldmentioning

confidence: 99%

“…Another strategy consists of adding a first layer of alkyl-thiol chain on the electrode, to which the capture molecules is further conjugated. This strategy was reported for the immobilization of antibodies [6,11,14,27,48,50,58,69,71,72,75,[77][78][79], ssDNA [13] and aptamers [8,80]. Numnuam et al also reported the immobilization of histones on gold electrodes for the detection of DNA traces [88].…”

Section: Self-assembled Monolayer (Sam) Formation On Metal Electrodesmentioning

confidence: 99%

Design and Preparation of Sensing Surfaces for Capacitive Biodetection

Robin

Gerber‐Lemaire

2022

Biosensors

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Despite their high sensitivity and their suitability for miniaturization, biosensors are still limited for clinical applications due to the lack of reproducibility and specificity of their detection performance. The design and preparation of sensing surfaces are suspected to be a cause of these limitations. Here, we first present an updated overview of the current state of use of capacitive biosensors in a medical context. Then, we summarize the encountered strategies for the fabrication of capacitive biosensing surfaces. Finally, we describe the characteristics which govern the performance of the sensing surfaces, along with recent developments that were suggested to overcome their main current limitations.

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“…in a wearable format) and overall a simplified practical implementation [ 14 , 16 ]. However, the capacitive measurement mode has been less studied with fewer capacitive biosensors reported to date [ 17 ]. Beyond the measurement itself, a crucial aspect of developing a new electrochemical detection technology is the nature of the surface, the ionic strength of the media, and the choice of the bioreceptor for recognition of the target [ 18 , 19 ].…”

Section: Introductionmentioning

confidence: 99%

Comparing nanobody and aptamer-based capacitive sensing for detection of interleukin-6 (IL-6) at physiologically relevant levels

Sánchez-Salcedo,

Miranda-Castro,

de-los-Santos-Álvarez

et al. 2023

Anal Bioanal Chem

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A major societal challenge is the development of the necessary tools for early diagnosis of diseases such as cancer and sepsis. Consequently, there is a concerted push to develop low-cost and non-invasive methods of analysis with high sensitivity and selectivity. A notable trend is the development of highly sensitive methods that are not only amenable for point-of-care (POC) testing, but also for wearable devices allowing continuous monitoring of biomarkers. In this context, a non-invasive test for the detection of a promising biomarker, the protein Interleukin-6 (IL-6), could represent a significant advance in the clinical management of cancer, in monitoring the chemotherapy response, or for prompt diagnosis of sepsis. This work reports a capacitive electrochemical impedance spectroscopy sensing platform tailored towards POC detection and treatment monitoring in human serum. The specific recognition of IL-6 was achieved employing gold surfaces modified with an anti-IL6 nanobody (anti-IL-6 VHH) or a specific IL-6 aptamer. In the first system, the anti-IL-6 VHH was covalently attached to the gold surface using a binary self-assembled-monolayer (SAM) of 6-mercapto-1-hexanol (MCH) and 11-mercaptoundecanoic acid. In the second system, the aptamer was chemisorbed onto the surface in a mixed SAM layer with MCH. The analytical performance for each label-free sensor was evaluated in buffer and 10% human serum samples and then compared. The results of this work were generated using a low-cost, thin film eight-channel gold sensor array produced on a flexible substrate providing useful information on the future design of POC and wearable impedance biomarker detection platforms.

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An impedimetric immunosensor for the selective detection of CD34+ T-cells in human serum

Cited by 8 publications

References 21 publications

Capacitive spectroscopy as transduction mechanism for wearable biosensors: opportunities and challenges

Capacitive spectroscopy as transduction mechanism for wearable biosensors: opportunities and challenges

Design and Preparation of Sensing Surfaces for Capacitive Biodetection

Comparing nanobody and aptamer-based capacitive sensing for detection of interleukin-6 (IL-6) at physiologically relevant levels

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