An impedance-coupled microfluidic device for single-cell analysis of primary cell wall regeneration

Chen, Lincai; Han, Ziyu; Fan, Xintong; Zhang, Shuaihua; Wang, Jiehua; Duan, Xuexin

doi:10.1016/j.bios.2020.112374

Cited by 22 publications

(19 citation statements)

References 55 publications

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“…By adding hormones to the circulating medium, the authors also observed the induction of leafy buds (Figure 4D) (Sakai et al, 2019). In another study, a microfluidic platform was designed with microelectrodes, coupled with electrical impedance spectroscopy, to study primary cell wall regeneration at the single-cell level (Chen et al, 2020) (Figure 4E). In this study, cells displaying a completely regenerated cell wall exhibited higher impedance values (i.e.…”

Section: The Rise Of Single Cell Approaches To Study Mechanicsmentioning

confidence: 98%

“…dielectric properties) compared to nascent protoplasts (Chen et al, 2020). This system also allows researchers to discriminate between several cell wall mutants and wild-type cells, thus providing a new tool for phenotypic analyses (Chen et al, 2020).…”

Section: The Rise Of Single Cell Approaches To Study Mechanicsmentioning

confidence: 99%

“…To circumvent this problem, it is possible to image undifferentiated cells, which have weaker cytoplasmic streaming than highly differentiated cells. Another solution, which was recently introduced to study endocytosis, is to reduce the dynamics of the system by rapidly lowering its temperature (Wang et al, 2020;Johnson et al, 2021). Finally, one can also use fast imaging systems such as spinning disk confocal microscopy, TIRF microscopy, or light sheet microscopy (Table 1).…”

Section: Cytoplasmic Streamingmentioning

confidence: 99%

“…Scale bar, 70 µm. E) Microscope image of a trapped Arabidopsis mesophyll cell (adapted fromChen et al, 2020). Flow direction was from left to right (red arrows).…”

mentioning

confidence: 99%

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Imaging the living plant cell: From probes to quantification

et al. 2021

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At the center of cell biology is our ability to image the cell and its various components, either in isolation or within an organism. Given its importance, biological imaging has emerged as a field of its own, which is inherently highly interdisciplinary. Indeed, biologists rely on physicists and engineers to build new microscopes and imaging techniques, chemists to develop better imaging probes, and mathematicians and computer scientists for image analysis and quantification. Live imaging collectively involves all the techniques aimed at imaging live samples. It is a rapidly evolving field, with countless new techniques, probes, and dyes being continuously developed. Some of these new methods or reagents are readily amenable to image plant samples, while others are not and require specific modifications for the plant field. Here, we review some recent advances in live imaging of plant cells. In particular, we discuss the solutions that plant biologists use to live image membrane-bound organelles, cytoskeleton components, hormones, and the mechanical properties of cells or tissues. We not only consider the imaging techniques per se, but also how the construction of new fluorescent probes and analysis pipelines are driving the field of plant cell biology.

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Section: The Rise Of Single Cell Approaches To Study Mechanicsmentioning

confidence: 98%

Section: The Rise Of Single Cell Approaches To Study Mechanicsmentioning

confidence: 99%

Section: Cytoplasmic Streamingmentioning

confidence: 99%

“…Scale bar, 70 µm. E) Microscope image of a trapped Arabidopsis mesophyll cell (adapted fromChen et al, 2020). Flow direction was from left to right (red arrows).…”

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confidence: 99%

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Imaging the living plant cell: From probes to quantification

et al. 2021

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“…For instance, protoplasts have been employed to assess the efficiency of CRISPR-associated protein 9 (Cas9) mutagenesis, bypassing or preceding stable transformation which can be time-consuming ( Lin et al, 2018 ; Hahn et al, 2020 ; Guyon-Debast et al, 2021 ; Nicolia et al, 2021 ). Another example is the adaptation of efficient and low-cost microfluidic techniques to perform spatiotemporal studies of plant protoplasts physiology during their development ( Sakai et al, 2019 ) and to apprehend the electrical resistance of CW-regenerated protoplasts ( Chen, 2020 ). Similarly, the usefulness of protoplasts for high-throughput RNA sequencing has also been put forward due to its many advantages over traditional RNA-seq.…”

Section: Challenges and Future Perspectivesmentioning

confidence: 99%

Protoplast: A Valuable Toolbox to Investigate Plant Stress Perception and Response

et al. 2021

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Plants are constantly facing abiotic and biotic stresses. To continue to thrive in their environment, they have developed many sophisticated mechanisms to perceive these stresses and provide an appropriate response. There are many ways to study these stress signals in plant, and among them, protoplasts appear to provide a unique experimental system. As plant cells devoid of cell wall, protoplasts allow observations at the individual cell level. They also offer a prime access to the plasma membrane and an original view on the inside of the cell. In this regard, protoplasts are particularly useful to address essential biological questions regarding stress response, such as protein signaling, ion fluxes, ROS production, and plasma membrane dynamics. Here, the tools associated with protoplasts to comprehend plant stress signaling are overviewed and their potential to decipher plant defense mechanisms is discussed.

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Design and 3D modeling investigation of a microfluidic electrode array for electrical impedance measurement of single yeast cells

et al. 2021

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High‐resolution microscopic imaging may cause intensive image processing and potential impact of light irradiation on yeast replicative lifespan (RLS). Electrical impedance spectroscopy (EIS) could be alternatively used to perform high‐throughput and label‐free yeast RLS assays. Prior to fabricating EIS‐integrated microfluidic devices for yeast RLS determination, systematic modeling and theoretical investigation are crucial for device design and optimization. Here, we report three‐dimensional (3D) finite‐element modeling and simulations of EIS measurement in a microfluidic single yeast in situ impedance array (SYIIA), which is designed by patterning an electrode matrix underneath a cell‐trapping array. SYIIA was instantiated and modeled as a 5 × 5 sensing array comprising 25 units for cell immobilization, culturing, and time‐lapse EIS recording. Simulations of yeast growing and budding in a sensing unit demonstrated that EIS signals enable the characterization of cell growth and daughter‐cell dissections. In the 5 × 5 sensing array, simulation results indicated that when monitoring a target cell, daughter dissections in its surrounding traps may induce variations of the recorded EIS signals, which could cause mistakes in identifying target daughter‐cell dissections. To eliminate the mis‐identifications, electrode array pitch was optimized. Therefore, the results could conduct the design and optimization of microfluidic electrode‐array‐integrated devices for high‐throughput and accurate yeast RLS assays.

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An impedance-coupled microfluidic device for single-cell analysis of primary cell wall regeneration

Cited by 22 publications

References 55 publications

Imaging the living plant cell: From probes to quantification

Imaging the living plant cell: From probes to quantification

Protoplast: A Valuable Toolbox to Investigate Plant Stress Perception and Response

Design and 3D modeling investigation of a microfluidic electrode array for electrical impedance measurement of single yeast cells

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