An immunoturbidimetric assay for canine C-reactive protein

Eckersall, P. D.; Conner, Joe; Harvie, Julie

doi:10.1007/bf00497786

Cited by 60 publications

(54 citation statements)

References 10 publications

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“…To the authors' knowledge, no data regarding precision studies of canine CRP measurements in CSF with other methods are available, so precision results could only be compared with those obtained with different methods that use serum for the determinations. Thus, CVs were similar to the ones reported in serum for a commercially available ELISA kit i,10,14 and for an immunoturbidimetric method, 4 except for the interassay CVs that were significantly lower than those obtained with the ELISA kit.…”

Section: Discussionsupporting

confidence: 80%

“…The limit of detection obtained with this method (7.1 3 10 26 mg/l) is much lower than that reported by the ELISA kit (0.1 mg/l) 14 and by the immunoturbidimetric assay (1 mg/l). 4 This extremely high sensitivity could represent a great advantage in comparison to former methodologies, since it will better detect low concentrations of CRP and might allow better monitoring of CNS diseases.…”

Section: Discussionmentioning

confidence: 99%

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Canine C-Reactive Protein Measurements in Cerebrospinal Fluid by a Time-Resolved Immunofluorimetric Assay

et al. 2011

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Abstract. In the current study, the quantification of C-reactive protein (CRP) in cerebrospinal fluid (CSF) of dogs using an adapted time-resolved immunofluorimetric assay (TR-IFMA) was investigated, as well as whether the assay could be used to detect the range of CRP concentrations found in different clinical situations. Intra-and interassay coefficients of variation were below 15% in all cases. The TR-IFMA measured the CRP values in a proportional and linear manner (r 5 0.99); also CRP concentrations measured in CSF and in serum were significantly correlated (r 5 0.80, P 5 0.003). The limit of detection of the method was 7.1 3 10 26 mg/l. The assay was able to detect differences in CRP concentrations in CSF of dogs with inflammatory disorders compared with dogs with spinal cord compression or idiopathic epilepsy. In conclusion, TR-IFMA constitutes a very sensitive, precise, and accurate method for the measurement of CRP concentrations in CSF.

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Section: Discussionsupporting

confidence: 80%

Section: Discussionmentioning

confidence: 99%

Canine C-Reactive Protein Measurements in Cerebrospinal Fluid by a Time-Resolved Immunofluorimetric Assay

et al. 2011

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“…Quantification of canine CRP in serum can be done using several assay formats,81, 82, 83, 84, 85, 86, 87, 88 all of which have a reference interval of approximately 0–8 mg/L. A high‐sensitivity CRP (hs‐CRP) assay with improved sensitivity for lower serum CRP concentrations89, 90 currently is not routinely available in veterinary medicine 91…”

Section: Biomarkers In Chronic Inflammatory Enteropathies Of Dogsmentioning

confidence: 99%

Clinical utility of currently available biomarkers in inflammatory enteropathies of dogs

Heilmann

Steiner

2018

Veterinary Internal Medicne

108

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Chronic inflammatory enteropathies (CIE) in dogs are a group of disorders that are characterized by chronic persistent or recurrent signs of gastrointestinal disease and histologic evidence of mucosal inflammation. These CIEs are classified as either food‐responsive, antibiotic‐responsive, or immunosuppressant‐responsive enteropathy. Patients not clinically responding to immunomodulatory treatment are grouped as nonresponsive enteropathy and dogs with intestinal protein loss as protein‐losing enteropathy. Disease‐independent clinical scoring systems were established in dogs for assessment of clinical disease severity and patient monitoring during treatment. Histopathologic and routine clinicopathologic findings are usually not able to distinguish the subgroups of CIE. Treatment trials are often lengthy and further diagnostic tests are usually at least minimally invasive. Biomarkers that can aid in defining the presence of disease, site of origin, severity of the disease process, response to treatment, or a combination of these would be clinically useful in dogs with CIE. This article summarizes the following biomarkers that have been evaluated in dogs with CIE during the last decade, and critically evaluates their potential clinical utility in dogs with CIE: functional biomarkers (cobalamin, methylmalonic acid, folate, α1‐proteinase inhibitor, immunoglobulin A), biochemical biomarkers (C‐reactive protein, perinuclear anti‐neutrophilic cytoplasmic antibodies, 3‐bromotyrosine, N‐methylhistamine, calprotectin, S100A12, soluble receptor of advanced glycation end products, cytokines and chemokines, alkaline phosphatase), microbiomic biomarkers (microbiome changes, dysbiosis index), metabolomic biomarkers (serum metabolome), genetic biomarkers (genomic markers, gene expression changes), and cellular biomarkers (regulatory T cells). In addition, important performance criteria of diagnostic tests are briefly reviewed.

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“…It should be noted that the observed correlation coefficient (r ¼ 0:94) between human CRP levels simultaneously measured by CRP-ELSA and immunoturbidimetry is superior to those previously achieved (r ¼ 0:88) for the comparative study of canine CRP detection by two immunometric methods, namely ELISA and canine CRP-specific immunoturbidimetry [14]. Furthermore, in the present study, the determination of canine CRP levels by CRP-ELSA correlates closely (r ¼ 0:89) with those obtained by canine CRPspecific ELISA.…”

Section: Discussionmentioning

confidence: 66%

Quantitative detection of C-reactive protein using phosphocholine-labelled enzyme or microspheres

Deegan

Walshe²,

Kavanagh

et al. 2003

Analytical Biochemistry

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C-reactive protein (CRP) is a positive, acute-phase protein. Plasma levels rise dramatically in response to tissue injury or inflammation and fall rapidly after recovery or treatment. Antibody-based human CRP test systems do not readily detect CRP from other animals due to the species specificity of antibodies directed against human CRP. Thus, generic systems for CRP detection, based solely on the interaction between CRP and phosphocholine (PC), have been developed. PC-bovine serum albumin (PC-BSA) conjugates were produced and either labeled with horseradish peroxidase to facilitate CRP detection in a CRP enzyme-linked sorbent assay (ELSA) or coupled to carboxy-modified microspheres to facilitate the nonenzymatic, turbidimetric detection of CRP. The CRP-ELSA is a competitive assay, where the total assay time is 45 min, the assay sensitivity is 1.06 mg/L CRP, and the dynamic range of the assay is 0-500 mg/L. When PC-BSA conjugate is covalently coupled to carboxylated microspheres, agglutination occurs in the presence of CRP, the extent of which depends on the quantity of CRP present in the sample. Total assay time is 5 min with a dynamic range of 25-500 mg/L. Both assay formats are capable of accurately detecting human CRP and the CRP-ELSA can detect canine CRP as a disease state indicator.

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An immunoturbidimetric assay for canine C-reactive protein

Cited by 60 publications

References 10 publications

Canine C-Reactive Protein Measurements in Cerebrospinal Fluid by a Time-Resolved Immunofluorimetric Assay

Canine C-Reactive Protein Measurements in Cerebrospinal Fluid by a Time-Resolved Immunofluorimetric Assay

Clinical utility of currently available biomarkers in inflammatory enteropathies of dogs

Quantitative detection of C-reactive protein using phosphocholine-labelled enzyme or microspheres

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