An immunosuppressive tryptophan-derived alkaloid from Lepidagathis cristata

Ravikanth, V.; Reddy, V. L. Niranjan; Ramesh, P.; Rao, T. Prabhakar; Diwan, Prakash V.; Khar, Ashok; Venkateswarlu, Y.

doi:10.1016/s0031-9422(01)00383-1

Cited by 40 publications

(39 citation statements)

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“…Plants of lepidagathis genus were used traditionally for the treatment of urinary calculi, dysuria, polyuria, fever, dysentery and uterine disorders (Madhavan et al, 2009). Other medicinal uses of lepidagathis plants include skin infections, malaria, migraine, cardiovascular diseases and gastric problems (HassanAbdallah et al, 2013;Mollik et al, 2009;Ravikanth et al, 2001;Richard et al, 2011). Previous studies have shown they possess excellent larvicidal, anti-inflammatory, analgesic, antipyretic and cytotoxic properties (Charoenchai et al, 2010;Obomanu et al, 2006;Richard et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of antiurolithiatic and antioxidant potential ofLepidagathis prostrata: APashanbhedplant

Devkar

Chaudhary

Adepu

et al. 2015

Pharmaceutical Biology

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Context: Oxidative stress acts as an essential mediator in the pathophysiology of urolithiasis. Lepidagathis prostrata Dalz. (Acanthaceae) is a Pashanbhed plant that is recommended for the management of urolithiasis; however, no scientific validation has been reported. Objectives: To evaluate the antiurolithiatic and antioxidant potential of L. prostrata. Materials and methods: Methanol extract (LPM) and fractions; petroleum ether (LPPE), ethyl acetate (LPEA), n-butanol (LPBU) and aqueous (LPAQ) were prepared. In vitro antiurolithiatic activity was evaluated by the capacity to inhibit calcium oxalate (CaOx) nucleation and aggregation at different concentrations of extract/fractions (0.04-3 mg/mL) for 30 min. Total phenol and flavonoid content and antioxidant potential were determined. A validated HPTLC method was performed to quantify lupeol and b-sitosterol. Results: LPEA exhibited the highest dose-dependent inhibition of CaOx nucleation (IC 50 : 336.23 ± 30.79 mg/mL) and aggregation (IC 50 : 149.63 ± 10.31 mg/mL), which was significantly (p50.05) better than standard Cystone Õ . The polar LPBU fraction was enriched with phenols (47.34 ± 0.19 mg GAE/g) and flavonoids (20.38 ± 0.05 mg QE/g), which correlates with its highest antioxidant potential in DPPH, ABTS, nitric oxide scavenging and iron chelating activities (IC 50 : 1.18-87.34 mg/mL). To our knowledge, this is the first study reporting the presence of lupeol and b-sitosterol in L. prostrata. Conclusion: The antiurolithiatic activity of L. prostrata is probably mediated through the inhibition of CaOx crystallization. In addition to its free radical scavenging and antioxidant activities, it would act as an excellent agent for the prevention of urolithiasis.

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Section: Introductionmentioning

confidence: 99%

Evaluation of antiurolithiatic and antioxidant potential ofLepidagathis prostrata: APashanbhedplant

Devkar

Chaudhary

Adepu

et al. 2015

Pharmaceutical Biology

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“…ARX., have been very frequently detected in housedust by low water activity (A w ) media such as dichloran 18% glycerol agar (DG-18) medium. 1) From the mycofloristic analyses of housedust samples from asthmatic patients' dwellings collected in Japan, 2) the total fungal counts cultivated on potato-dextrose agar (PDA) medium, which had high A w value, were 1.3ϫ10 5 CFU (colony formatting units) per 1 g of sample, in which Penicillium sp. (3ϫ10 4 CFU) was detected as main fungal flora along with a small amount of xerophilic fungi; A. restrictus (10 2 CFU), while the total fungal counts cultivated on DG-18 medium, which had low A w value, were 1.6ϫ10 6 CFU per 1 g of sample, in which A. restrictus (6ϫ10 5 CFU), Cladosporium sphaerospermum PENZIG (2ϫ10 5 CFU), W. sebi (10 5 CFU), and Penicillium sp.…”

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“…Cristatin A (3) had originally been isolated as an immunosuppressive substance from the whole plant of Lepidagathis cristata WILLD. (Acanthaceae), 5) but no report of its isolation from the fungal sources had been made, whereas asperglaucide (4), identified as N-benzoyl-L-phenylalanyl-L-phenylalaninol acetate, had been isolated from Aspergillus glaucus LINK: FR.…”

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confidence: 99%

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Two New Dioxopiperazine Derivatives, Arestrictins A and B, Isolated from Aspergillus restrictus and Aspergillus penicilloides

Itabashi

Matsuishi

Hosoe

et al. 2006

CHEMICAL & PHARMACEUTICAL BULLETIN

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In the course of searching for bioactive substances, two new dioxopiperazine derivatives, arestrictins A (1) and B (2), were isolated along with the known dioxopiperazine, cristatin A (3), and the known peptide, asperglucide (4), from the organic extract of the xerophilic fungi, Aspergillus restrictus and Aspergillus penicilloides. The absolute structures of 1 and 2, except for the configuration of the secondary alcohol in 1, were established by spectroscopic and chemical investigation. The absolute configuration of cristatin A (3) was also determined.

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“…The plants such as Achillea talagonica [20], Plantago ovata [21], Boerhaavia diffusa [22], Acorus calamus [23], Prunella vulgaris [12], Typha angustifolia [13], and Siegesbeckia orientalis [14] have been shown to possess immunosuppressive activity. Various active principles such as triptolide and tripdiolide from the root of Tripterygium wifordii [24], cyclolinopeptide B from the seeds of Linum usitatissimum [25], cynaropicrin from the root of Saussurea lappa [26] [27], cristatin A from the whole plant of Lepidagathis cristata [28], 6-hydroxythiobinupharidine, 6,6'- Significant differences with OVA groups were designated as *: P < 0.05, **: P < 0.01, and ***: P < 0.001. CsA: cyclosporin A (positive drug).…”

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confidence: 99%

Immunosuppressive Activity of Semen Persicae Ethanol Extract on Specific Antibody and Cellular Response to Ovalbumin in Mice

Zhang

Qin

Sun

2006

C&B

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The immunosuppressive activity of the ethanol extract of Semen Persicae (EESP) was studied with respect to specific antibody and cellular response to ovalbumin (OVA) in mice. The effects of EESP on mice splenocyte proliferation in vitro were measured. EESP significantly suppressed concanavalin A (ConA)- and lipopolysaccharide (LPS)-stimulated splenocyte proliferation in vitro in a concentration-dependent manner. Furthermore, the effects of EESP at three dose levels on the humoral and cellular immune responses in the OVA-immunized mice were examined. ICR Mice were immunized subcutaneously with OVA on day 0 and 14. Starting on the day of immunization, the mice were administered intraperitoneally with EESP at a single dose of 0.25, 0.5, and 1.0 mg, and cyclosporin A (CsA, positive drug) at a single dose of 0.1 mg at intervals of 7 days. On day 28, mitogen- and OVA-induced splenocyte proliferation and OVA-specific antibody level in serum were measured. EESP significantly decreased ConA-, LPS-, and OVA-induced splenocyte proliferation in the OVA-immunized mice at the dose of 1.0 mg. Meanwhile, the OVA-specific serum IgG, IgG1, and IgG2b antibody levels in the OVA-immunized mice were markedly reduced by EESP in a dose-dependent manner. The results suggest that EESP could suppress the cellular and humoral immune response in mice, and deserve further research to be developed as immunosuppressant.

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An immunosuppressive tryptophan-derived alkaloid from Lepidagathis cristata

Cited by 40 publications

References 7 publications

Evaluation of antiurolithiatic and antioxidant potential ofLepidagathis prostrata: APashanbhedplant

Evaluation of antiurolithiatic and antioxidant potential ofLepidagathis prostrata: APashanbhedplant

Two New Dioxopiperazine Derivatives, Arestrictins A and B, Isolated from Aspergillus restrictus and Aspergillus penicilloides

Immunosuppressive Activity of Semen Persicae Ethanol Extract on Specific Antibody and Cellular Response to Ovalbumin in Mice

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