An immunosuppressive peptide from the horsefly inhibits inflammation by repressing macrophage maturation and phagocytosis

Abstract

Ectoparasites repress host immune responses while they obtain nutrition from their hosts. Understanding the immunosuppressive mechanisms between ectoparasites and their hosts will provide new strategies to develop potential immunosuppressive drugs against immune disorder diseases. Previously, we have discovered that a small peptide, immunoregulin HA, from the horsefly (Hybomitra atriperoides) may play an immunosuppressive role in rat splenocytes. However, the targeting cells and detailed mechanisms of immunoregulin HA in immunosuppressive reactions are not well defined. Here, we show that immunoregulin HA reduces the secretion of proinflammatory cytokines upon lipopolysaccharide (LPS) stimulation. Interestingly, we discover that the major cytokines repressed by immunoregulin HA are secreted by macrophages, rather than by T cells. Furthermore, immunoregulin HA inhibits macrophage maturation and phagocytosis. Mechanically, the activations of c-JUN N-terminal kinase and extracellular signal-regulated kinase upon LPS stimulation are decreased by immunoregulin HA. Consistently, immunoregulin HA treatment exhibits an anti-inflammatory activity in a mouse model of adjuvant-induced paw inflammation. Taken together, our data reveal that immunoregulin HA conducts the anti-inflammatory activity by blocking macrophage functions.

Advances in Modern Pharmacology Research of Tabanus

“…It was found that cecropin-TY1, immunomodulatory protein HA and immunoregulin TPs in Tabanus SGE had strong immune and anti-inflammatory effects [2,6,7] . Immunomodulatory protein HA inhibits the maturation and phagocytosis of macrophages [6] . Tabimmunregulins increased the production of IL-10 and decreased IFN-γ secretion [2] .…”