An Immunological Approach to the Evaluation of Welfare in Holstein Frisian cattle

Amadori, Massimo; Archetti, I. L.; Frasnelli, M.; Bagni, M.; Olzi, E.; Caronna, G.; Lanteri, Marion C.

doi:10.1111/j.1439-0450.1997.tb00982.x

Cited by 42 publications

(41 citation statements)

References 15 publications

(6 reference statements)

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“…Pernthaner et al (1997) admitem também ocorrer trombocitopenia e Adler et al (1996) descrevem decréscimo no fator de necrose tumoral alfa. Feitosa et al (2001) Amadori et al (1997) e Bonnizzi et al (2003.…”

Section: Discussionunclassified

Dosagem de lisozima para avaliação da resposta imune em bezerros

García

Chate

Porto

et al. 2005

RBCV

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ResumoO presente trabalho visou verificar a eficiência da dosagem de lisozima como indicador de imunidade em bovinos, valendo-se de animais naturalmente acometidos de processo diarréico. Para tanto, foram utilizados bezerros com até três meses de idade, criados em manejo semi-intensivo e divididos em três grupos experimentais (I -sem diarréia, II -com diarréia mas sem sintomas sistêmicos, III -com diarréia e sintomas sistêmicos). Foram colhidas amostras sangüíneas de cada animal, que foram processadas para a realização da dosagem de gamaglobulinas por eletroforese, dosagem de IgG por imunodifusão radial, contagem de linfócitos e dosagem de lisozima. Os resultados encontrados mostraram existir linfopenia nos animais do grupo III, mas não foi verificada hipogamaglobulinemia na eletroforese ou na imunodifusão radial. Não foram encontradas diferenças estatisticamente significantes no nível de lisozima entre os grupos estudados. Não foi possível demonstrar correlação entre a dosagem de lisozima e os outros indicadores preconizados no presente experimento. Todavia, embora no presente experimento não se tenha encontrado uma correlação positiva entre o teor de lisozima e parâmetros de resposta imunitária, os resultados obtidos sugerem que novos trabalhos devam ser realizados para que tal parâmetro seja validado como indicador biológico.Palavras-chave: lisozima, resposta imune, bezerros. AbstractThe purpose of this work was to evaluate the serum lysozyme concentration as an indicator of immune response in bovine, considering naturally affected animals with diarrheic process. The experiment was conducted using 3 months old calves, raised in semi-intensive system and allocated into 3 groups with 10 animals each one (I -no diarrhea, II -diarrhea but no systemic symptoms, III -diarrhea and systemic symptoms). Blood samples were collected from each animal and processed by electrophoresis to verify the gamma-globulin content. The IgG was measured by radial immunodiffusion. Other measurements included blood lymphocyte count and serum lysozyme concentration. The results showed lymphopenia in animals of group III, but no differences were found in gamma-globulin content, as measured by electrophoresis and radial immunodiffusion. No differences were found in the lysozyme content in all groups analyzed and it was not possible to demonstrate a correlation between the lysozime content and the other indicators studied in this experiment. However, although it was not found this positive correlation, the results suggest that new works have to be performed to validate this parameter as a biological indicator.

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Section: Discussionunclassified

Dosagem de lisozima para avaliação da resposta imune em bezerros

García

Chate

Porto

et al. 2005

RBCV

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“…Serum lysozyme was measured by the lyso-plate assay (Osserman and Lawlor, 1966) and its concentration value was expressed in µg/ml.The serum bactericidal activity was valued following conventional procedures (Amadori et al, 1997). Statistical analysis were performed using the Student's T-test and expressed as mean ±stan-dard deviation (SD).…”

Section: Methodsmentioning

confidence: 99%

Investigations of some parameters of natural immunity in meat turkeys reared outdoors

Franciosini

Proietti

Moscati

et al. 2009

Italian Journal of Animal Science

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The progression of some parameters of natural immunity in meat turkey reared outdoors was investigated. The levels of the haemolytic complement were lower than those obtained in commercial turkeys and in turkeys reared in a controlled environment during one of our previous investigations. The weather conditions could have influenced the trend of the haemolytic complement in turkeys selected for high meat production and kept outside, underlining the possible importance of the rearing system.

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“…Whenever animals are forced to deal with severe, prolonged coping reactions with a considerable energy expense, welfare suffers and a serious depression of the immune system results as one of the negative outcomes. In this scenario, clinical immunology tests (complement, lysozyme, serum bactericidal activity) reveal a substantial decrease in the ability of the immune system to deal with environmental pathogens, which paves the way to opportunistic microbial infections (Amadori, 1997). Interestingly, the above functions relate to the innate immune system, which does not have memory and acts irrespective of antigenic specificities by recognition of conserved microbial PAMPs.…”

Section: The Immune System Is Affected By the Stress Responsementioning

confidence: 99%

Immune system response to stress factors

Amadori¹,

Stefanon

Sgorlon

et al. 2009

Italian Journal of Animal Science

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This review highlights fundamental mechanisms of the stress response and important findings as to how the immune system is affected and affects, in turn, such a response. The crucial link between stress response and energy metabolism is dealt with as well. The effector mechanisms in the stress response are remarkably similar for both infectious and non-infectious stimuli, albeit differently modulated. "Psychosensitive stimuli/behavioural response" and "Antigenic stimuli/immune response" are indeed two subsystems of a unitary, integrated complex aimed at providing optimal conditions for the host's survival and adaptation. The interaction between the immune system and the stress/inflammation complex has led to the development of a diversified network of cytokines and chemokines in vertebrate animals. The cytokine response can be mounted in different forms and extent by the host after exposure to both infectious and non-infectious stimuli. In this conceptual framework, microbial infections are just one category of stressing agents, which modulate the cytokine response for a better performance of the innate and adaptive immune responses. The response to infectious and non-infectious stress leads to a metabolic shift that enhances energy, amino acids and micronutrients consumption. The influence of each nutrient on different aspects of immune function is not easy to define, but it is becoming clear that many nutrients have defined roles in the immune response and, accordingly, their requirements are changed to support optimal immune function. Therefore, impairment of immune functions may arise from intakes of nutrients below or above these modified ranges of requirements.

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An Immunological Approach to the Evaluation of Welfare in Holstein Frisian cattle

Cited by 42 publications

References 15 publications

Dosagem de lisozima para avaliação da resposta imune em bezerros

Dosagem de lisozima para avaliação da resposta imune em bezerros

Investigations of some parameters of natural immunity in meat turkeys reared outdoors

Immune system response to stress factors

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