An immunohistochemical study
on the regulation of estrogen receptor ? by estradiol
in the endometrium of the immature ewe

Meikle, A.; Bielli, Alejandro; Masironi, Britt; Pedrana, G.; Wang, Hong; Forsberg, M.; Sahlin, Lena

doi:10.1051/rnd:2000102

Cited by 24 publications

(20 citation statements)

References 31 publications

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“…A high dose of phytoestrogen genistein induced down-regulation of ERα mRNA and protein levels in human breast cancer cell lines [29]. Changes in ER seem to be very rapid, since down-regulation of ER occurred within 6-12 h in endometrium cells of prepubertal lambs treated with estrogen [30]. This report supports our presumption that PM phytoestrogens may bind and modulate ER in sexual skin.…”

Section: Discussionsupporting

confidence: 83%

Effect of Pueraria mirifica on the Sexual Skin Coloration of Aged Menopausal Cynomolgus Monkeys

Trisomboon

Malaivijitnond

Cherdshewasart

et al. 2006

Journal of Reproduction and Development

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Abstract. To investigate the estrogenic effect of Pueraria mirifica (PM), a Thai herbal plant that contains many phytoestrogens, sexual skin coloration was studied in cynomolgus monkeys. Aged menopausal monkeys were divided into three groups. Each group (n=3) was fed 10, 100, or 1,000 mg of PM daily. The treatment schedule was divided into three periods, a 30-day pre-treatment period, 90-day treatment period, and 60-day post-treatment period. The results show that the sexual skin exhibited reddish coloration within 24 h after PM-treatment and remained this way for the first half of the PMfeeding period. The changes in sexual skin coloration were not dose-dependent. The present results indicate that PM had estrogenic action by increasing reddish sexual skin coloration in aged menopausal monkeys. Key words: Macaca fascicularis, Phytoestrogens, Pueraria mirifica, Sexual skin (J. Reprod. Dev. 52: [537][538][539][540][541][542] 2006) t has been recognized that the roots of Pueraria mirifica (PM), a Thai herbal plant, contain many kinds of phytoestrogens, such as genistein, daidzein, and coumestrol, which are also found in soy plants and soy products, and miroestrol, deoxymiroestrol, peurarin, and mirificin [1][2][3][4]. The phytoestrogens from soys [5,6] and PM roots [7][8][9] have been reported to have estrogenic actions in both in vivo and in vitro studies. The estrogenic effect of PM was examined in vitro using MCF-7 cell proliferation and HepG2 cell transient transfection assay [7,8]. PM had a dual effect on the growth of MCF-7 human mammary adenocarcinoma cells after 4 days of incubation, i.e. stimulating growth at a low dose and suppressing it with a high dose [8]. PM also showed estrogenic activity on a human hepatoma cell line, HepG2 cells, containing estrogen receptor and luciferase reporter gene [7]. Administration of PM produced cornified cells, g r o w t h o f t h e e n d o m e t r i u m g l a n d , a n d proliferation of spiral arteries in ovariectomized rats [9]. Furthermore, we reported in a recent investigation that daily feeding of PM had an estrogenic effect by suppressing gonadotropin and estrogen secretions in aged female cynomolgus monkeys [10].The influence of estrogen on the sexual skin area around the perineum to the base of the tail has been

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Section: Discussionsupporting

confidence: 83%

Effect of Pueraria mirifica on the Sexual Skin Coloration of Aged Menopausal Cynomolgus Monkeys

Trisomboon

Malaivijitnond

Cherdshewasart

et al. 2006

Journal of Reproduction and Development

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“…These results were expected since the down-regulation of estrogen receptor α following estrogen treatment has been shown in endometrial glands of ovariectomized macaques (Wang et al 2002), in endometrial and stromal cells of immature ewes (Meikle et al 2000), and in the human endometrial carcinoma cell line ECC-1 (Dardes et al 2002). Fig.…”

Section: Immunocytochemical Characterizationsupporting

confidence: 61%

The phytoestrogens daidzein and genistein enhance the insulin-stimulated sulfate uptake in articular chondrocytes

et al. 2008

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Clinical observations have suggested a relationship between osteoarthritis and a changed estrogen metabolism in menopausal women. Phytoestrogens have been shown to ameliorate various menopausal symptoms. Proteoglycans (PG) consisting of low and high sulfated glycosaminoglycans (GAG) are the main components of articular cartilage matrix, and their synthesis is increased by insulin in growth plate cartilage. We have investigated whether GAG synthesis and sodium [35S]sulfate incorporation in female bovine articular chondrocytes are affected by daidzein, genistein, and/or insulin. For comparative purposes, estradiol incubations were performed. Articular chondrocytes were cultured in monolayers at 5% O2 and 5% CO2 in medium containing serum for 7 days followed by the addition of 10(-11) M-10(-4) M daidzein, genistein, 17beta-estradiol, or 5 microg/ml insulin in a serum-free culture phase of 2 days. Photometrically analyzed GAG synthesis was significantly suppressed by high doses (10(-5) M-10(-4) M) of daidzein, genistein, and 17beta-estradiol. Although insulin raised the sodium [35S]sulfate uptake significantly, different concentrations of daidzein, genistein, or 17beta-estradiol showed no significant effects. However, the stimulating effect of insulin on sulfate incorporation was enhanced significantly after preincubation of cells with 10(-11) M-10(-5) M daidzein or 10(-9) M-10(-5) M genistein but not by 17beta-estradiol. In view of the risks of long-term estrogen replacement therapy, further experiments should clarify the potential benefit of phytoestrogens and insulin in articular cartilage metabolism.

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“…By contrast, estrogens in the culture medium proved to be strong repressors of ER-alpha production by hTERT-EECs. Down-regulation of ER-alpha following estrogen treatment has recently been shown in endometrial glands of ovariectomized macaques (Wang et al 2002), in endometrial epithelial and stromal cells of immature ewes (Meikle et al 2000) and in the human endometrial carcinoma cell line ECC-1 (Dardes et al 2002). hTERT-EECs produced a functional ER-alpha that was clearly responsive to estrogen or the synthetic estrogenic compound DES, as demonstrated by a strong induction of luciferase using a ERE-luciferase reporter plasmid.…”

Section: Steroid Hormone-responsive Htert-eecs · S Hombach-klonisch Amentioning

confidence: 94%

Regulation of functional steroid receptors and ligand-induced responses in telomerase-immortalized human endometrial epithelial cells

Hombach‐Klonisch¹,

Kehlen²,

Fowler³

et al. 2005

Journal of Molecular Endocrinology

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Information on the regulation of steroid hormone receptors and their distinct functions within the human endometrial epithelium is largely unavailable. We have immortalized human primary endometrial epithelial cells (EECs) isolated from a normal proliferative phase endometrium by stably transfecting the catalytic subunit (hTERT) of the human telomerase complex and cultured these hTERT-EECs now for over 350 population doublings. Active hTERT was detected in hTERT-EECs employing the telomerase repeat amplification assay protocol. hTERT-EECs revealed a polarized, non-invasive epithelial phenotype with apical microvilli and production of a basal lamina when grown on a three-dimensional collagen-fibroblast lattice. Employing atomic force microscopy, living hTERT-EECs were shown to produce extracellular matrix (ECM) components and ECM secretion was modified by estrogen and progesterone (P4). hTERT-EECs expressed inducible and functional endogenous estrogen receptor-alpha (ER-alpha) as demonstrated by estrogen response element reporter assays and induction of P4 receptor (PR). P4 treatment down-regulated PR expression, induced MUC-1 gene activity and resulted in increased ER-beta transcriptional activity. Gene activities of cytokines and their receptors interleukin (IL)-6, leukemia inhibitory factor (LIF), IL-11 and IL-6 receptor (IL6-R), LIF receptor and gp130 relevant to implantation revealed a 17 beta-estradiol (E2)-mediated up-regulation of IL-6 and an E2-and P4-mediated up-regulation of IL6-R in hTERT-EECs. Thus, hTERT-EECs may be regarded as a novel in vitro model to investigate the role of human EECs in steroid hormone-dependent normal physiology and pathologies, including implantation failure, endometriosis and endometrial cancer.

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An immunohistochemical study on the regulation of estrogen receptor ? by estradiol in the endometrium of the immature ewe

Cited by 24 publications

References 31 publications

Effect of Pueraria mirifica on the Sexual Skin Coloration of Aged Menopausal Cynomolgus Monkeys

Effect of Pueraria mirifica on the Sexual Skin Coloration of Aged Menopausal Cynomolgus Monkeys

The phytoestrogens daidzein and genistein enhance the insulin-stimulated sulfate uptake in articular chondrocytes

Regulation of functional steroid receptors and ligand-induced responses in telomerase-immortalized human endometrial epithelial cells

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