An Immunohistochemical Study on Ki-67 Expression in Squamous Cell Carcinomas of Cervix With Clinicopathological Correlation

Abstract

BackgroundCervical carcinoma is one of the most prevalent cancers affecting women worldwide. Studies on Ki-67 expression in cervical lesions had focused mainly on the intraepithelial lesions of the cervix and not much on invasive carcinomas. The few studies published so far on Ki-67 expression in invasive cervical carcinomas have shown inconsistent results on the association of Ki-67 with various clinicopathological prognostic factors. Aims and objectivesTo assess Ki-67 expression in cervical carcinomas and to compare it with various clinicopathological prognostic factors. Materials and methodsFifty cases of invasive squamous cell carcinoma (SCC) were included in the study. Histological patterns and grades were identified and noted in these cases after microscopic examination of the histological sections. Immunohistochemical (IHC) staining with anti-Ki-67 was done and scored from 1+ to 3+. This score was compared with clinicopathological prognostic factors like clinical stage, histological pattern, and grade. ResultAmong the 50 cases of SCC, 41 showed keratinizing pattern (82%) and nine showed non-keratinizing pattern (18%). Four were in stage I, 25 were in stage II, and 21 were in stage III. Overall, 34 (68%) cases had Ki-67 score 3+, 11 (22%) had Ki-67 score 2+, and five (10%) had Ki-67 score 1+. Ki-67 score of 3+ was the most common score in keratinizing SCC (75.6%), poorly differentiated carcinomas (76.2%), and stage III cases (81%). ConclusionWe observed statistically significant correlation of Ki-67 expression with higher clinical stage, keratinizing tumours, and poorly differentiated tumours (p<0.05) indirectly implying the poor prognostic significance of this marker.

Expression and Clinical Significance of Ki-67, CD10, BCL6, MUM1, c-MYC, and EBV in Diffuse Large B Cell Lymphoma Patients

“…In addition, despite the lack of significant association between Ki-67 expression and clinicopathologic features in patients with DLBCL observed in this study, it should be noted that such associations have been reported in various malignancies, including DLBCL. [51][52][53] For instance, in a study conducted by Hashmi et al, patients with non-GCB subtype demonstrated a significantly higher Ki67 index when compared with those with the GCB subtype of DLBCL. Additionally, a positive association was found between c-MYC expression and a higher Ki-67 index.…”

PD-L1 expression in squamous cervical carcinomas of Mozambican women living with or without HIV

“…Ki67 was scored as positive when brown staining was present in the nucleus at any intensity. The positive staining was further categorized in three-grade scale in a semi-quantitative manner as: Score 1: 10–30% stained cells (Low proliferation); Score2: 30–50% stained cells (moderate proliferation); Score3: > 50% (high proliferation) 70 .…”

Prognostic and clinicopathological value of Ki-67 in patients with oesophageal squamous cell carcinoma: a systematic review and meta-analysis

“… 6 Ki-67 is related to the cell cycle and mitosis and is commonly used in clinical settings to measure cellular proliferation. 7 Ki-67 expression measured through immunohistochemical (IHC) staining has been identified as a biomarker to predict the prognosis of different cancer types, such as breast cancer, 8 multiple myeloma, 9 cervical cancer, 10 diffuse malignant peritoneal mesothelioma 11 and oral squamous cell carcinoma. 12 Many studies have examined the role of Ki-67 in predicting ESCC prognosis, but no consistent findings have been obtained.…”