An immunohistochemical study of the ontogeny of the horizontal cell in the rat retina using an antiserum against spot 35 protein, a Novel purkinje cell‐specific protein, as a marker

Kondo, Hisatake; Yamamoto, Miyuki; Yamakuni, Tohru; Takahashi, Yasuo

doi:10.1002/ar.1092220115

Cited by 17 publications

(8 citation statements)

References 17 publications

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“…In accordance with earlier data (Ramon y Cajal, 1960;Hinds and Hinds, 1979;Shaw and Weber, 1983;Schnitzer and Rusoff, 1984;Prada et al, 1984;Redburn and Madtes, 1986;Osborne et al, 1986;Reichenbach et al, 1987;Kondo et al, 1988;Versaux-Botteri et al, 1989;Araki and Kimura, 1991), we found that embryonic HC are often vertically oriented and may be found in inner retinal zones. HC processes reaching into the inner plexiform layer and cells with dendrites in both plexiform layers (Fig.…”

Section: Developmental Stages Of Rabbit A-type Hcsupporting

confidence: 94%

Development of A‐type (axonless) horizontal cells in the rabbit retina

Scheibe

Schnitzer

Röhrenbeck

et al. 1995

J of Comparative Neurology

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The development of A-type horizontal cells (HC) was studied in the rabbit retina between embryonic day (E)24 and adulthood [the day of birth was called postnatal day (P)1 and corresponds to E31-32]. The cells were visualized by several methods 1) by immunolabeling with antibodies to neurofilament 70,000 (NF-70kD), 2) by immunolabeling with antibodies to a calcium binding protein (CaBP-28kD), 3) by two different methods of silver impregnation, and 4) by histochemical demonstration of NADH-diaphorase activity. Most methods labeled A-type HC only in the dorsal retina; thus, our study is restricted to HC of this region. HC densities were determined at each developmental stage. The cells were drawn at scale, and size, quotient of symmetry, and topographical orientation of dendritic trees were studied by image analysis. The growth of HC dendritic fields was correlated with data on the postnatal local retinal expansion, which is known to be driven by the intraocular pressure (after cessation of retinal cell proliferation at P9). This expansion was evaluated in an earlier paper (Reichenbach et al. [1993] Vis. Neurosci. 10:479-498) by using local subpopulations of Müller cells as "markers" of distinct topographic regions of the retinae. After E24, when the final number of HC is established, we can discriminate three distinct developmental stages of A-type HC. During the first stage, between E24 and E27, the young cells are often vertically oriented and may extend their first short dendrites within (the primordia of) both plexiform layers. The irregular HC mosaic at E24 shows a significant difference to all other stages. The second stage begins after birth when the dendritic trees of the cells are already restricted to the outer plexiform layer. Between P3 and P9, their dendritic trees enlarge more than the surrounding retinal tissue expands, and the coverage factor almost doubles from 2.5 to 4.4. The third stage occurs after P9 when the growth rate of dendritic tree areas corresponds to that of the local retinal tissue expansion caused by "passive stretching" of the postmitotic tissue, and the coverage factor remains constant. This is compatible with the view that mature synaptic connections of A-type HC are mostly established after the first week of life and are then maintained.

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Section: Developmental Stages Of Rabbit A-type Hcsupporting

confidence: 94%

Development of A‐type (axonless) horizontal cells in the rabbit retina

Scheibe

Schnitzer

Röhrenbeck

et al. 1995

J of Comparative Neurology

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“…Neither spot 35-nor CaBP-28k-immunoreacfivity has so far been detected in ordinary astrocytes, Schwann or satellite cells in the nervous system (Stichel, et al, 1986;Furness et al, 1988;Kondo et al, 1988;Philippe & Droz, 1988;DiFiglia et aI., 1989;Sloviter, 1989). This, together with the phylogenetic consideration of the occurrence of the calcium-binding protein described above, suggests that the calciumbinding spot 35 protein in the pineal interstitial cells is involved in some yet to be determined calciummediated functions that are not shared with ordinary astrocytes.…”

Section: Discussionmentioning

confidence: 66%

Expression of immunoreactivity for Ca-binding protein, spot 35 in the interstitial cell of the rat pineal organ

et al. 1990

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In the rat pineal organ numerous stellate cells exhibited intense immunoreactivity for calcium-binding spot 35 protein. Because of their peculiar shape and ultrastructure, identical to those of intrapineal S-100-immunoreactive cells, the spot 35-immunoreactive stellate cells were identified as the interstitial cells. The comparison of the morphology and population density of spot 35-, S-100-, and GFAP (glial fibrillar acidic protein)-immunoreactive cells, suggests that spot 35-immunoreactive cells represent a major subpopulation of the interstitial cells, all of which are S-100-immunoreactive and generally considered to be of glial nature, while GFAP-immunoreactive cells represent a minor subpopulation of the interstitial cells located in the proximal part close to the pineal stalk. This is the first report describing the occurrence of the calcium-binding protein in cells of glial nature.

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“…8), the numbers of immunoreactive cells in the neuroblast layer was reduced, whereas the inner plexiform layer was thicker and displayed a reduction in the density of immunoreactive processes ramifying through it. At this time point, many presumptive horizontal cells (defined by reference to previous descriptions of these cells in the developing rat retina; Kondo et al 1988) were clearly immunonegative for D-aspartate. By P7 (Fig.…”

Section: Uptake Of D-aspartate In Neonatal Retinaementioning

confidence: 99%

Changing patterns of spatial buffering of glutamate in developing rat retinae are mediated by the Müller cell glutamate transporter GLAST

Pow

Barnett

1999

Cell and Tissue Research

103

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The patterns of expression of the glutamate transporter GLAST were compared with the patterns of uptake of exogenous D-aspartate, which is a substrate for all glutamate transporters. At postnatal day 0, fine radial processes and end feet of presumptive Müller cells were weakly immunoreactive for GLAST. At postnatal day 3, intense labelling was associated with astrocytes enveloping newly formed blood vessels on the vitread surface of the retina. Between postnatal days 7 and 10, there was a rapid increase in the intensity of labelling in the Müller cells but clear stratification of GLAST-immunoreactive processes in the inner plexiform layer was not observed until postnatal day 14. By comparison, D-aspartate uptake was initially associated with a wide variety of cellular elements including most neuroblasts, presumptive Müller cells, and astrocytes associated with blood vessels but was absent from the somata of many neurons in the ganglion cell layer and amacrine cell layer. There was a gradual contraction in the numbers of cells that were able to take up D-aspartate, such that, by adulthood, uptake was restricted mainly to Müller cells and astrocytes. We conclude that, during early retinal development, the low levels of GLAST expression by Müller cells permit D-aspartate, and by inference, glutamate, to permeate the retina freely, thus allowing uptake by other glutamate transporters on other cell types. As the retina matures, increased expression of GLAST by Müller cells restricts the access of D-aspartate to other cellular compartments in the retina. This changing pattern of spatial buffering of glutamate by GLAST probably has significant implications regarding our understanding of the role of glutamate during processes such as retinal synaptogenesis.

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An immunohistochemical study of the ontogeny of the horizontal cell in the rat retina using an antiserum against spot 35 protein, a Novel purkinje cell‐specific protein, as a marker

Cited by 17 publications

References 17 publications

Development of A‐type (axonless) horizontal cells in the rabbit retina

Development of A‐type (axonless) horizontal cells in the rabbit retina

Expression of immunoreactivity for Ca-binding protein, spot 35 in the interstitial cell of the rat pineal organ

Changing patterns of spatial buffering of glutamate in developing rat retinae are mediated by the Müller cell glutamate transporter GLAST

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