An Immunohistochemical Study of the Cytoskeletal Proteins in the Testis of the Sunda porcupine (<i>Hystrix javanica</i>)

Budipitojo, Teguh; Sasaki, Motoki; Nurliani, Anni; Ariana, .; Mulyani, Guntari Titik; Kitamura, Nobuo

doi:10.3106/ms2017-0052

Cited by 2 publications

(2 citation statements)

References 21 publications

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“…Intermediate filaments are one of the three major elements of the cell cytoskeleton and are associated with cell junctions at Sertoli-Sertoli cell junctions. The localization of vimentin and other non-typical intermediate filaments as glial fibrillary acid protein (GFAP) and neurofilaments (NF) were targeted in the Sertoli cell cytoplasm ( Aumüller et al, 1992 ; Davidoff et al, 1999 ; Budipitojo et al, 2018 ). Vimentin filaments distribution changed with the stage of spermatogenesis, with the shortest filaments found during early spermiation stages ( Show et al, 2003 ).…”

Section: Background: Mammalian Spermatogenesis and Btbmentioning

confidence: 99%

What do we know about blood-testis barrier? current understanding of its structure and physiology

Luaces

Toro-Urrego

Otero‐Losada

et al. 2023

Front. Cell Dev. Biol.

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Blood-testis barrier (BTB) creates a particular compartment in the seminiferous epithelium. Contacting Sertoli cell-Sertoli cell plasma membranes possess specialized junction proteins which present a complex dynamic of formation and dismantling. Thus, these specialized structures facilitate germ cell movement across the BTB. Junctions are constantly rearranged during spermatogenesis while the BTB preserves its barrier function. Imaging methods are essential to studying the dynamic of this sophisticated structure in order to understand its functional morphology. Isolated Sertoli cell cultures cannot represent the multiple interactions of the seminiferous epithelium and in situ studies became a fundamental approach to analyze BTB dynamics. In this review, we discuss the contributions of high-resolution microscopy studies to enlarge the body of morphofunctional data to understand the biology of the BTB as a dynamic structure. The first morphological evidence of the BTB was based on a fine structure of the junctions, which was resolved with Transmission Electron Microscopy. The use of conventional Fluorescent Light Microscopy to examine labelled molecules emerged as a fundamental technique for elucidating the precise protein localization at the BTB. Then laser-scanning confocal microscopy allowed the study of three-dimensional structures and complexes at the seminiferous epithelium. Several junction proteins, like the transmembrane, scaffold and signaling proteins, were identified in the testis using traditional animal models. BTB morphology was analyzed in different physiological conditions as the spermatocyte movement during meiosis, testis development, and seasonal spermatogenesis, but also structural elements, proteins, and BTB permeability were studied. Under pathological, pharmacological, or pollutant/toxic conditions, there are significant studies that provide high-resolution images which help to understand the dynamic of the BTB. Notwithstanding the advances, further research using new technologies is required to gain information on the BTB. Super-resolution light microscopy is needed to provide new research with high-quality images of targeted molecules at a nanometer-scale resolution. Finally, we highlight research areas that warrant future studies, pinpointing new microscopy approaches and helping to improve our ability to understand this barrier complexity.

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Section: Background: Mammalian Spermatogenesis and Btbmentioning

confidence: 99%

What do we know about blood-testis barrier? current understanding of its structure and physiology

Luaces

Toro-Urrego

Otero‐Losada

et al. 2023

Front. Cell Dev. Biol.

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“…Desmin intermediate filament is expressed in the cytoplasm of all muscle types and endothelial cells [13], while smooth muscle actin (SMA) is a contractile actin isoform present in smooth muscle cells [14] and myofibroblasts [15]. Immunohistochemical studies have demonstrated that desmin and SMA are localized in the peritubular smooth muscle layer of the epididymal duct of the Sunda porcupine [16], as well as in the testicular excurrent duct system of adult duck, emu, fowl, Japanese quail, masked weaver, ostrich, and turkey [3,4,6]. Desmin and SMA play an important role in the structural and functional support of the contractile action of smooth muscle cells [13,15].…”

Section: Introductionmentioning

confidence: 99%

Immunohistochemical Changes in the Testicular Excurrent Duct System of Healthy, Male Japanese Quail (Coturnix coturnix japonica) Observed at 4, 6–7, 12, and 52 Weeks of Age

Ibrahim

Williams

Botha

2022

IJMS

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The immunolocalization of the cytoskeletal and the extracellular matrix proteins was investigated in the testicular excurrent duct system of healthy Japanese quail at 4, 6–7, 12 and 52 weeks of age. TdT dUTP Nick End Labeling (TUNEL) assay was used to assess apoptotic cell formation. The epithelia of the testicular excurrent duct system in birds of all age groups displayed various immunolabeling intensities and localization of cytokeratin 5 and beta-tubulin, while α-SMA was observed in epithelia only of 4-week-old birds. In all age groups, vimentin immunostaining was observed in the rete testes and efferent ductular epithelia, but not in the epididymal duct unit. The periductal smooth muscle cells of the excurrent duct system displayed variably intense immunopositivity with cytokeratin 5, desmin, fibronectin, α-SMA, and beta-tubulin. Furthermore, beta-tubulin and vimentin immunolabeled endothelial cells and fibroblasts with various intensities, while fibronectin immunostained extracellular matrices surrounding these cells. TUNEL-positive apoptotic cells were observed in the rete testes and efferent ductular epithelia, with increased frequency (p < 0.001) in 52-week-old birds. The study serves as a baseline normal for this region in healthy birds at 4, 6–7, 12, and 52 weeks of age, for comparison in future similar immunohistochemical studies involving environmental toxins affecting this region.

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An Immunohistochemical Study of the Cytoskeletal Proteins in the Testis of the Sunda porcupine (Hystrix javanica)

Cited by 2 publications

References 21 publications

What do we know about blood-testis barrier? current understanding of its structure and physiology

What do we know about blood-testis barrier? current understanding of its structure and physiology

Immunohistochemical Changes in the Testicular Excurrent Duct System of Healthy, Male Japanese Quail (Coturnix coturnix japonica) Observed at 4, 6–7, 12, and 52 Weeks of Age

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