An immunohistochemical study of regenerating newt retinas

Morphological and Functional Organization of ON and OFF Pathways in the Adult Newt Retina

“…Therefore, classification of amacrine cells is highly dependent on the parameters chosen. We have already shown neurochemical diversity of newt amacrine cells with respect to GABA (Chiba et al, 1997), acetylcholine (Cheon and Saito, 1999), and several neuropeptides (Negishi et al, 1992). In newt retina, like other urodele species (Gläsener, et al, 1988), GABAergic amacrine cells are most abundant and located in the INL and GCL in two to three cell rows close to the IPL.…”

“…The antibody was used at a dilution of 1:300 in a mixture of 2% normal goat serum and 0.3% Triton X-100 in PBS. The procedure of PKC immunohistochemistry is described above and also in the previous papers (Negishi, et al ., 1992;Saito et al ., 1994). For double labeling with synaptotagmin and PKC, sections were first incubated with the anti-PKC and then with anti-synaptotagmin as described above.…”

“…At least two immunohistochemical markers, PKC and RB-1, for retinal bipolar cells have been identified in the adult newt retina (Negishi, et al ., 1992;Saito et al ., 1994). Fig.…”

“…The procedure of RB-1 immunohistochemistry is described in the previous papers (Negishi, et al ., 1992;Saito et al ., 1994). Because RB-1 and anti-synaptotagmin were made from the same host species, we used Fab fragments of affinity-purified secondary antibodies for double labeling of the primary antibodies.…”

Rx for tissue restoration: Regenerative biology and medicine

“…The depigmented RPE cells continue to express this antigen as they proliferate. Expression of RPE-1 is extinguished as the cells express NR cell markers (Negeshi et al, 1992;Mtashov, 1996). This pattern suggests that dedifferentkting RPE cells may continue to express genes specific for the RPE as they undergo transdetermination to patterns of gene activity characteristic of NR cells.…”