An immunohistochemical study of HLA-DR and α1-antichymotrypsin-positive cells in the pulp of human non-carious and carious teeth

Izumi, Toshio; Kobayashi, Ieyoshi; Okamura, Kazuhiko; Matsuo, Keitaro; Kiyoshima, Tamotsu; Ishibashi, Yukihide; Inoue, Hiroshi; Sakaf, Hidetaka

doi:10.1016/s0003-9969(96)00075-1

Cited by 25 publications

(16 citation statements)

References 7 publications

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“…The class II MHC antigen is called Ia antigen in the rat, and can be recognized by an OX6 antibody (McMaster and Williams, 1979). To date, these OX6-immunopositive cells have been reported to show characteristic reaction patterns under various experimental conditions such as tooth grinding (Kamal et al, 2000;Ohshima et al, , 1996, tooth replantation (Rungvechvuttivittaya et al, 1998;Shimizu et al, 2000), and carious teeth (Izumi et al, 1996;Kamal et al, 1997;Sakurai et al, 1999;Yoshiba et al, 1996). Interestingly, under experimental conditions, the class II MHC-positive cells have been suggested to have diverse functions in odontoblast differentiation in the rat dental pulp in addition to ordinary antigen presentation .…”

Section: Introductionmentioning

confidence: 99%

Pulpal regeneration after cavity preparation, with special reference to close spatio‐relationships between odontoblasts and immunocompetent cells

Ohshima

Nakakura-Ohshima

Takeuchi

et al. 2003

Microscopy Res & Technique

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The regeneration process of the odontoblast cell layer incident to tooth injury, especially its relationship with immunocompetent cells in pulp healing, has not been fully understood. The purpose of the present study was to clarify this relationship between odontoblasts and immunocompetent cells in the process of pulp regeneration following cavity preparation in rat molars by immunocytochemistry for heat shock protein (Hsp) 25 as well as class II major histocompatibility complex (MHC) molecules. In untreated control teeth, intense Hsp 25-immunoreactivity was found in the cell bodies of odontoblasts and their processes within the predentin, whereas class II MHC-positive cells were predominantly located beneath the odontoblast cell layer. Cavity preparation caused the destruction of the odontoblast layer to form an edematous lesion and the shift of class II MHC-positive cells with the injured odontoblasts toward the pulp core at the affected site. Some damaged odontoblasts without apparent cytoplasmic processes, round in profile, retained the immunoreactivity for Hsp25, suggesting the survival of a part of the odontoblasts against artificial external stimuli. Twelve hours after cavity preparation, numerous class II MHC-positive cells appeared along the pulp-dentin border and extended their processes deep into the exposed dentinal tubules. By postoperative 72 hours, newly differentiated odontoblasts with Hsp 25-immunoreactivity were arranged at the pulp-dentin border, but the class II MHC-positive cells moved from the pulp-dentin border to the subodontoblastic layer. These findings indicate that the time course of changes in the expression of Hsp 25-immunoreactivity reflects the regeneration process of odontoblasts. The functional roles of Hsp 25-positive odontoblasts and immunocompetent cells such as class II MHC-positive cells in the process of pulp regeneration after cavity preparation are discussed in conjunction with our previous experimental data.

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Section: Introductionmentioning

confidence: 99%

Pulpal regeneration after cavity preparation, with special reference to close spatio‐relationships between odontoblasts and immunocompetent cells

Ohshima

Nakakura-Ohshima

Takeuchi

et al. 2003

Microscopy Res & Technique

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“…The involvement of class II major histocompatibility complex (MHC)-positive cells in the initial defense reaction after tooth injury has been demonstrated in experimental and/or pathological conditions such as tooth grinding (Ohshima et al , 1996 and carious teeth (Yoshiba et al 1996;Izumi et al 1996, Kamal et al 1997Sakurai et al 1999). A recent investigation on tooth replantation has also identified a drastic accumulation of pulpal immunocompetent cells at the postoperative early stage, and postulated that they are strategically situated at the outermost portion of the injured pulp as an immunosurveillance (Rungvechuttivittaya et al 1998).…”

Section: Introductionmentioning

confidence: 99%

Responses of immunocompetent cells in the dental pulp to replantation during the regeneration process in rat molars

et al. 2000

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Responses of immunocompetent cells to tooth replantation during the regeneration process of the dental pulp in rat molars were investigated by immunocytochemistry using antibodies to class II major histocompatibility complex (MHC) molecules (OX6 antibody), monocyte/macrophage lineage cells (ED1 antibody) and protein gene product 9.5 (PGP 9.5), as well as by histochemical reaction for periodic acid-Schiff (PAS). Tooth replantation caused an increase in both the number of OX6- and ED1-positive cells and their immunointensity in the replanted pulp, but almost all PGP 9.5-immunoreactive nerves diminished in the initial stages. By postoperative day 3, many OX6- and ED -immunopositive cells had accumulated along the pulp-dentin border to extend their cytoplasmic processes into the dentinal tubules in successful cases. Once reparative dentin formation had begun after postoperative day 7, OX6- and ED1-immmunopositive cells became scattered in the odontoblast layer, while reinnervation was found in the coronal pulp. The temporal appearance of these immunocompetent cells at the pulp-dentin border suggests their participation in odontoblast differentiation as well as in initial defense reactions during the pulpal regeneration process. On postoperative day 14, the replanted pulp showed three regeneration patterns: (1) reparative dentin, (2) bone-like tissue formation, and (3) an intermediate form between these. In all cases, PAS-reactive cells such as polymorphonuclear leukocytes (PML) and mesenchymal cells occurred in the pulp space. However, the prolonged stagnation of inflammatory cells was also discernible in the latter two cases. Thus, the findings on PAS reaction suggest that the migration of the dental follicle-derived cells into the pulp space and the subsequent total death of the proper pulpal cells are decisive factors for eliciting bone-like tissue formation in the replanted pulp.

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“…The involvement of class II MHC-positive cells in the dental pulp in the initial defense reaction after tooth injury has been confirmed in the case of tooth grinding and in carious teeth (Yoshiba et al 1996;Izumi et al 1996;Kamal et al 1997). Furthermore, current reports suggest that the class II MHC-positive cells play a role in odontoblast differentiation in the dental pulp under experimental conditions , and might also play an inductive role in the differentiation, migration and/or activation of the odontoclasts and cementoblast-like cells in the coronal pulp of human deciduous teeth during physiological root resorption (Kannari et al 1998).…”

Section: Introductionmentioning

confidence: 82%

The distribution and ultrastructure of class II MHC-positive cells in human dental pulp

Ohshima

Maeda

Takano

1999

Cell and Tissue Research

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The distribution and ultrastructure of class II major histocompatibility complex (MHC)-positive cells were investigated in human dental pulp, employing immunohistochemistry using an anti-human leukocyte antigen (HLA)-DR-monoclonal antibody. HLA-DR-immunopositive cells, appearing spindle-like or dendritic in profile, were densely distributed throughout the dental pulp. Under the electron microscope, these cells exhibited various sizes of vesicles containing clear or opaque contents, multivesicular bodies and characteristic fine tubulovesicular structures in their cytoplasm. Some reactive cells possessed coated pits and vesicles including electron-dense materials, indicating an active endocytosis. At the periphery of the pulp tissue, the HLA-DR-immunopositive cells were predominantly situated in the subodontoblastic layer, with some located in the odontoblast layer and/or predentin and extending their cytoplasmic processes into the dentinal tubules. Cell processes of these cells occasionally made contact with several odontoblast processes in the same way as the nerve fibers in the predentin. These cells never contained the typical phagosomes frequently observed in the HLA-DR-immunoreactive macrophages in the subodontoblastic layer and the pulp core. The results suggest that the HLA-DR-immunopositive cells in the odontoblast layer and/or predentin have some regulatory function on the odontoblasts under physiological conditions, in addition to their involvement in the initial defense reaction after tooth injury.

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An immunohistochemical study of HLA-DR and α1-antichymotrypsin-positive cells in the pulp of human non-carious and carious teeth

Cited by 25 publications

References 7 publications

Pulpal regeneration after cavity preparation, with special reference to close spatio‐relationships between odontoblasts and immunocompetent cells

Pulpal regeneration after cavity preparation, with special reference to close spatio‐relationships between odontoblasts and immunocompetent cells

Responses of immunocompetent cells in the dental pulp to replantation during the regeneration process in rat molars

The distribution and ultrastructure of class II MHC-positive cells in human dental pulp

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