An immunohistochemical assessment of cellular proliferation markers in head and neck squamous cell cancers

Jh, Kearsley; Furlong, Kevin; Cooke, RA; Waters, M. J.

doi:10.1038/bjc.1990.184

Cited by 99 publications

(57 citation statements)

References 40 publications

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“…The immunocytochemical pattern of EGF receptor expression in the tissues from which the cell lines were derived in the present study was differentiation related in that there was marked staining in basal epithelial cells and diminished reactivity in areas of squamous differentiation; this confirms previous observations (Partridge et al, 1988;Sakai et al, 1990;Kearsley et al, 1990;Shirasuna et al, 1991 were evident in the present study, and it may be that radioimmunoassays would clarify this situation.…”

Section: Discussionsupporting

confidence: 81%

Epidermal growth factor and transforming growth factor α characteristics of human oral carcinoma cell lines

Prime

Game²,

Matthews³

et al. 1994

Br J Cancer

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Section: Discussionsupporting

confidence: 81%

Epidermal growth factor and transforming growth factor α characteristics of human oral carcinoma cell lines

Prime

Game²,

Matthews³

et al. 1994

Br J Cancer

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“…Extensive scoring methods have been designed in an attempt to increase the objectivity of these parameters [34][35][36][37]. Other ancillary parameters, such as mitotic index, DNA content, Ki-67, proliferation cell nuclear antigen, and bromodeoxyuridine labelling index, may be used in an attempt to identify new prognostic indicators [38,39]. But, due to financial constraints, availability of advanced parameters along with advanced technology for carrying out research in the developing countries, like us, are the real limitations.…”

Section: Discussionmentioning

confidence: 99%

Nuclear pleomorphism-based cytopathological grading in human oral neoplasm

Mohanta¹,

Prafulla²

2017

Russ. Open Med. J.

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Objective -The objective of the present study is record the nuclear pleomorphism in various stages of oral carcinogenesis and to analyse their utility in cytopathological grading for early detection of human oral cancer Material and Methods -In this hospital based case-control study, oral site, age-group and sex-matched 272 subjects (136 cases and 136 normal healthy individuals) were included. Scraped exfoliated cytosmears were collected from the affected oral site of the subjects and smearing was done in the pre-cleaned-coded glass-slides. Two such slides were prepared from each subject. The cytosmears were immediately fixed in aceto-alcohol (1 part of glacial acetic acid: 3 part of absolute ethyl alcohol) fixative. One set of the slide was stained with Papanicolaou's stain and the other set was counter-stained with Giemsa's Solution for cytopathological analysis. Test of proportion (ztest) was followed and the critical ratio (z-value) was calculated for the test of significance. Results -Nuclear pleomorphism in the form of round, oval, spindle, elongated fiber as well as irregular shapes were mostly observed in oral squamous cells during different stages of carcinogenesis. Appearance of such nuclear pleomorphism in human oral neoplasm may be considered as a sign of cellular alternation in general and index of oral carcinogenesis in particular. In the present study, the frank malignant cases mimic to be either premalignant lesions or benign/carcinoma in situ were detected on the basis of nuclear pleomorphismbased cytopathological grading and so an increasing trend was observed from precancerous lesions to malignant cases due to shifting of numbers. Diagnostic tests also indicated that the Sensitivity was calculated to be 83.5%, Specificity was 100%, positive predictive value (PPV) was 100%, negative predictive value (NPV) was 30% and the accuracy was found to be 84.6%. Therefore, the nuclear pleomorphismbased cytopathological grading system makes itself an ideal screening test for early detection of human oral cancer. Conclusion -Pattern of nuclear pleomorphism corresponding to various cytological atypias is a common feature observed during different stages of oral carcinogenesis and thus, it has a practical implication in grading and early detection of oral cancer.

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“…A severe overexpression of EGFR may correspond with a wide expression of receptors on the cell surface, leading to a constitutive activation of the downstream effectors, with generation of a marked, proliferative ligand-mediated signal. In fact, a specific clinical study has demonstrated a positive correlation between the intensity of EGFR expression and the nuclear proliferative index, Ki-67, in head-and-neck SCC (21). Tumour cells increase their proliferative activity, and the repopulation effect derived from this mechanism may counteract the effects of radiotherapy (22,23).…”

Section: Biology Of Nmsc and The Rationale For Using Targeted Therapymentioning

confidence: 99%

Concomitant cetuximab and radiation therapy: A possible promising strategy for locally advanced inoperable non-melanoma skin carcinomas

Scarpati

Perri

Pisconti

et al. 2016

Molecular and Clinical Oncology

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Abstract. Non-melanoma skin cancers (NMSCs) include a heterogeneous group of malignancies arising from the epidermis, comprising squamous cell carcinoma (SCC), basal cell carcinoma (BCC), Merkel cell carcinoma and more rare entities, including malignant pilomatrixoma and sebaceous gland tumours. The treatment of early disease depends primarily on surgery. In addition, certain patients present with extensive local invasion or metastasis, which renders these tumours surgically unresectable. Improving the outcome of radiotherapy through the use of concurrent systemic therapy has been demonstrated in several locally advanced cancer-treatment paradigms. Recently, agents targeting the human epidermal growth factor receptor (EGFR) have exhibited a consolidated activity in phase II clinical trials and case series reports. Cetuximab is a monoclonal antibody that binds to and completely inhibits the EGFR, which has been revealed to be up-regulated in a variety of SCCs, including NMSCs. The present review aimed to summarize the role of anti-EGFR agents in the predominant types of NMSC, including SCC and BCC, and focuses on the cetuximab-based studies, highlighting the biological rationale of this therapeutic option. In addition, the importance of the association between cetuximab and radiotherapy for locally advanced NMSC is discussed.

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An immunohistochemical assessment of cellular proliferation markers in head and neck squamous cell cancers

Abstract: Summary Prognostic information is essential for the evaluation, judgement and optimal treatment of patients with squamous cell cancers (SCCs)

Cited by 99 publications

References 40 publications

Epidermal growth factor and transforming growth factor α characteristics of human oral carcinoma cell lines

Epidermal growth factor and transforming growth factor α characteristics of human oral carcinoma cell lines

Nuclear pleomorphism-based cytopathological grading in human oral neoplasm

Concomitant cetuximab and radiation therapy: A possible promising strategy for locally advanced inoperable non-melanoma skin carcinomas

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