An Immunogenetically Defined and Immunodominant Trypanosoma Cruzi Antigen

R, Ramos Galván; Juri, María Cecilia; Ramos, Amparo; Hoecker, G; Lavandero, Sergio; Peña, Pedro Rafael Jiménez; Morello, Antonio; Repetto, Yolanda; Aguillón, Juan Carlos; Ferreira, Arturo

doi:10.4269/ajtmh.1991.44.314

Cited by 22 publications

(17 citation statements)

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“…In contrast, we have shown that epimastigotes display an important amount of TcCRT in the cytoplasmic compartment (45). Although the presence of TcCRT in supernatants from trypomastigote and epimastigote cultures, on the epimastigote surface, and in sera of T. cruzi-infected individuals and mice, should be addressed, at present it is not possible to estimate the real TcCRT concentrations in the relevant parasite in vivo microenvironment.…”

Section: Discussionmentioning

confidence: 81%

“…Native TcCRT is immunogenic in humans (43,44) and mice (45). Thus, most, if not all, T. cruzi-seropositive humans display variable amounts of anti-TcCRT Igs.…”

mentioning

confidence: 99%

“…TcCRT from another T. cruzi strain has also been characterized (33,47). TcCRT is a 45-kDa immunodominant (45), dimorphic Ag (different parasite clones present alternative 45-and 43-kDa forms), with multiple gene copies located in a variable number of chromosomes (32,43). Importantly, certain regions within the TcCRT S (TcS) domain are up to 80% identical with HuCRT.…”

mentioning

confidence: 99%

“…cruzi CRT (TcCRT) was first isolated in our laboratory and named Tc45 (43)(44)(45)(46). We have cloned, sequenced, and expressed a TcCRT gene (32).…”

mentioning

confidence: 99%

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The Classical Activation Pathway of the Human Complement System Is Specifically Inhibited by Calreticulin from Trypanosoma cruzi

Ferreira¹,

Valck²,

Sánchez

et al. 2004

The Journal of Immunology

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124

141

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The high resistance of Trypanosoma cruzi trypomastigotes, the causal agent of Chagas’ disease, to complement involves several parasite strategies. In these in vitro studies, we show that T. cruzi calreticulin (TcCRT) and two subfragments thereof (TcCRT S and TcCRT R domains) bind specifically to recognition subcomponents of the classical and lectin activation pathways (i.e., to collagenous tails of C1q and to mannan-binding lectin) of the human complement system. As a consequence of this binding, specific functional inhibition of the classical pathway and impaired mannan-binding lectin to mannose were observed. By flow cytometry, TcCRT was detected on the surface of viable trypomastigotes and, by confocal microscopy, colocalization of human C1q with surface TcCRT of infective trypomastigotes was visualized. Taken together, these findings imply that TcCRT may be a critical factor contributing to the ability of trypomastigotes to interfere at the earliest stages of complement activation.

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Section: Discussionmentioning

confidence: 81%

“…Native TcCRT is immunogenic in humans (43,44) and mice (45). Thus, most, if not all, T. cruzi-seropositive humans display variable amounts of anti-TcCRT Igs.…”

mentioning

confidence: 99%

mentioning

confidence: 99%

“…cruzi CRT (TcCRT) was first isolated in our laboratory and named Tc45 (43)(44)(45)(46). We have cloned, sequenced, and expressed a TcCRT gene (32).…”

mentioning

confidence: 99%

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The Classical Activation Pathway of the Human Complement System Is Specifically Inhibited by Calreticulin from Trypanosoma cruzi

Ferreira¹,

Valck²,

Sánchez

et al. 2004

The Journal of Immunology

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124

141

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“…Thus, when ␤ 2 -microglobulin binds to the major histocompatibility complex (MHC) class I ␣ chain, the partially folded ␣␤ 2 -microglobulin heterodimer dissociates from calnexin and binds calreticulin. [14][15][16] The findings previously reported by this laboratory, 7,17,18 have characterized the immunogenicity and diagnostic value of T. cruzi calreticulin, then known as Tc45. These results plus those reported here open a wide range of possibilities to study the role of this immunologically relevant molecule in parasite infectivity and its potential immunoprophylactic and immunodiagnostic value.…”

Section: Introductionmentioning

confidence: 96%

Tc45, a dimorphic Trypanosoma cruzi immunogen with variable chromosomal localization, is calreticulin.

Aguillón

Ferreira²,

Pérez³

et al. 2000

The American Journal of Tropical Medicine and Hygiene

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Abstract. We demonstrate that Tc45, a polypeptide described as an immunogenetically restricted Trypanosoma cruzi antigen in mice, is calreticulin, a dimorphic molecule encoded by genes with variable chromosomal distribution. Previously we showed that IgG from A.SW (H2 s ) mice immunized with T. cruzi trypomastigotes or epimastigotes and sera from infected humans recognize Tc45, a 45 kD parasite polypeptide. Herein we describe the cloning, sequencing, and expression of the Tc45 gene. A 98% homology in the deduced amino acid sequence was found with a T. cruzi calreticulin-like molecule and 41% with Leishmania donovani and human calreticulin. In the T. cruzi CL Brener clone and in the Tulahuén strain, the gene is located in two and four chromosomes, respectively. Calreticulin was detected in several T. cruzi clones, in the Tulahuén strain, and in T. rangeli, displaying alternative 43 and 46 kD forms.

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Comparative effect of human andTrypanosoma cruzicalreticulin in wound healing

Arias

Sepúlveda

Bravo

et al. 2012

J Tissue Eng Regen Med

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In orthopaedics, the use of factors that enhance granulation tissue formation and prevent or delay new bone regeneration is sometimes desirable. Calreticulin (CRT), a unique endoplasmic reticulum luminal Ca(2+) -binding chaperone widely distributed in eukaryotic cells, is involved in many cellular functions. Among them, CRT has an important influence in cutaneous wound healing and diverse processes associated with cutaneous repair, inhibition of angiogenesis, promotion of cell adhesion and antitumour effect. One of the molecules involved in several aspects of the host-parasite interplay is Trypanosoma cruzi calreticulin (TcCRT), which is highly homologous to human calreticulin (HuCRT). Here, recombinant (r)HuCRT and rTcCRT are compared on their abilities to affect fibroblast behaviour in a scratch plate assay, and wound healing in in vivo skin rat models. In molar terms, rTcCRT is three orders of magnitude more efficient than rHuCRT in increasing proliferation and migration of human fibroblasts in vitro. A similar effect was observed in vivo on rat skin wounds and inhibition of bone gap bridging in rabbit unicortical bone osteotomies.

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An Immunogenetically Defined and Immunodominant Trypanosoma Cruzi Antigen

Cited by 22 publications

References 0 publications

The Classical Activation Pathway of the Human Complement System Is Specifically Inhibited by Calreticulin from Trypanosoma cruzi

The Classical Activation Pathway of the Human Complement System Is Specifically Inhibited by Calreticulin from Trypanosoma cruzi

Tc45, a dimorphic Trypanosoma cruzi immunogen with variable chromosomal localization, is calreticulin.

Comparative effect of human andTrypanosoma cruzicalreticulin in wound healing

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