An immunofluorescence assay for the detection of parvovirus B19 IgG and IgM antibodies based on recombinant viral antigen

Brown, Caroline; Bussel, Mario J.A.W.M. van; Wassenaar, Alfred L. M.; Elsacker-Niele, Anne-Marie W. van; Weiland, H. T.; Salimans, M. M. M.

doi:10.1016/0166-0934(90)90007-3

Cited by 46 publications

(18 citation statements)

References 17 publications

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“…Anti-parvovirus B 19 IgM assays have previously been described which have used a variety of techniques such as R1 A, FITC enhanced ELISA, IgM capture assay and immunofluorescence [4,5,7,18]. Furthermore it is known that monoclonal antibodies have been made against the VP 1 and VP 2 capsid proteins of B 19 [19].…”

Section: Discussionmentioning

confidence: 99%

Two anti-parvovirus B 19 IgM capture assays incorporating a mouse monoclonal antibody specific for B 19 viral capsid proteins VP 1 and VP 2

O’Neill

Coyle

1992

Archives of Virology

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During an outbreak of parvovirus B 19 in 1989 in Northern Ireland, 7580 blood donors were screened for B 19 antigen. Two units screened positive, one of which was obtained for use as viral antigen. A monoclonal antibody (R92F6) made against this antigen was specific for B 19 capsid proteins VP 1 and VP 2. The monoclonal antibody was used in the development of 2 anti-B 19 IgM capture enzyme assays. These used a conventional substrate (O-phenylene diamine) and a chemiluminescent signal reagent. There was excellent concordance between the 2 assays. A total of 403 patients sera were tested and 65 sera were positive in each assay.

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Section: Discussionmentioning

confidence: 99%

Two anti-parvovirus B 19 IgM capture assays incorporating a mouse monoclonal antibody specific for B 19 viral capsid proteins VP 1 and VP 2

O’Neill

Coyle

1992

Archives of Virology

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“…They were initially tested for recent infection with cytomegalovirus, Epstein-Barr virus, and hepatitis B virus as possible causes of thrombocytopenia. Now they were tested for parvovirus B19-specific IgG and IgM antibodies using an immunofluorescence assay [3] or an ELISA [16]. These tests appear to be of comparable diagnostic value (unpublished data).…”

Section: Methodsmentioning

confidence: 97%

Parvovirus B19 infection and idiopathic thrombocytopenic purpura*

et al. 1996

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The potential association of human parvovirus B19 infection with idiopathic thrombocytopenic purpura (ITP) was studied. All 60 adult patients presenting with ITP at the University Hospital Rotterdam - Dijkzigt during a 12-year period (41 with acute ITP, 19 with chronic ITP) were included. Patient files were retrospectively analyzed. Stored serum samples were tested for parvovirus B19-specific IgG and IgM anti-bodies, and for parvovirus B19 DNA. In only one patient (1.7%) was evidence of recent B19 infection found. Parvovirus B19 is not a frequent cause of adult ITP and should be tested for only when there are other indications of possible parvovirus B19 involvement.

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“…B19 antibody detection (IgM and IgG) by IFA using baculovirus infected cells expressing VP1 was first described by Brown et al (1990b). There is now a commercial IFA test developed by Biotrin International Ltd (Ireland) based on VP1 expressed in Sf9 cells.…”

Section: Discussionmentioning

confidence: 99%

Anti-VP1 and anti-VP2 antibodies detected by immunofluorescence assays in patients with acute human parvovirus B19 infection

Pereira¹,

Paula²,

Cubel³

et al. 2001

Mem. Inst. Oswaldo Cruz

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An immunofluorescence assay for the detection of parvovirus B19 IgG and IgM antibodies based on recombinant viral antigen

Cited by 46 publications

References 17 publications

Two anti-parvovirus B 19 IgM capture assays incorporating a mouse monoclonal antibody specific for B 19 viral capsid proteins VP 1 and VP 2

Two anti-parvovirus B 19 IgM capture assays incorporating a mouse monoclonal antibody specific for B 19 viral capsid proteins VP 1 and VP 2

Parvovirus B19 infection and idiopathic thrombocytopenic purpura*

Anti-VP1 and anti-VP2 antibodies detected by immunofluorescence assays in patients with acute human parvovirus B19 infection

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