An immunocytochemical and ultrastructural study of endocrine cells in the gut of a teleost fish, Sparus auratus L

Elbal, M.T.; Agulleiro, B.

doi:10.1016/0016-6480(86)90067-5

Cited by 54 publications

(63 citation statements)

References 46 publications

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“…The presence of abundant vesicles implicated in pinocytotic processes immediately below the terminal web of the intestinal absorptive cells of S. auratus, has previously been described by electron microscopy (6). In keeping with this, WGA-positive granules have been visualized in such a region in the present study.…”

Section: Gallus Gallus (Chicken) Small Intestinesupporting

confidence: 88%

Characterization of glycoconjugates in the intestinal mucosa of vertebrates by means of lectin histochemistry.

Madrid

Ballesta

Castells

et al. 1989

Acta Histochem. Cytochem

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The glycoproteins of the intestinal mucosa of five vertebrate species including fishes (Sparus auratus), amphibians (Rang perezi), reptiles (Testudo graeca), birds (Gallus gallus) and mammals (Mesocricetus auratus) were studied by means of methods of lectin histochemistry. Goblet cells of the intestine showed different lectin binding patterns depending upon the location in the villi. Thus, goblet cells located in the intestinal glands presented a different reactivity from that of the goblet cells located at the tip of the villi. Differences in lectin stainings between small and large intestines were more marked in higher than in lower vertebrates. WGA binding sites were the most abundant throughout the intestinal mucosa of the animals studied. Golgi areas of the different cell types studied were frequently reactive for DBA and SBA. The results obtained in the Brunner's glands of the hamster were similar to those obtained in other mammals, suggesting a common mucin composition. In the hamster intestinal glands, Con-A stained the Paneth cells.

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Section: Gallus Gallus (Chicken) Small Intestinesupporting

confidence: 88%

Characterization of glycoconjugates in the intestinal mucosa of vertebrates by means of lectin histochemistry.

Madrid

Ballesta

Castells

et al. 1989

Acta Histochem. Cytochem

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“…A capillary network is present in the lamina propria of the mucosal layer under the epithelium. This stomach morphology has been described for several teleostean fishes, including Gasterosteus aculeatus (Hale, 1965), Monopterus albus (Liem, 1967), Esox lucius (Bucke, 1971), Perca fluviatilis (Noaillac-Depeyre and Gas, 1978), Ictalurus punctatus (Sis et al, 1979), Anguilla anguilla (Clarke and Witcomb, 1980), Girella tricuspidata (Anderson, 1986), Sparus auratus (Elbal and Agulleiro, 1986a), Anarhichas lupus (Helberg and Bjerkas, 2000), and Misgurnus mizolepis (Park and Kim, 2001).…”

Section: Discussionmentioning

confidence: 92%

“…Three types of EC in the stomach of Hypostomus with DCV of diameter 88 -389 nm are located only within the gastric glands and one type of EC is open. EC of type I in the stomach of Hypostomus possess DCV with a mean diameter of 132 nm, which resemble DCV in EC of type I in the respiratory intestine of Corydoras aeneus and are similar to DCV found in EC producing catecholamines in other fish species: Perca fluviatilis and Ameiurus nebulosus (NoaillacDepeyre and Gas, 1982), and Sparus auratus (Elbal and Agulleiro, 1986b). EC of type II in the stomach of Hypostomus have one type of DCV.…”

Section: Discussionmentioning

confidence: 92%

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Morphology of the air‐breathing stomach of the catfish Hypostomus plecostomus

Podkowa

Goniakowska-Witalińska

2003

Journal of Morphology

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Histological and ultrastructural investigations of the stomach of the catfish Hypostomus plecostomus show that its structure is different from that typical of the stomachs of other teleostean fishes: the wall is thin and transparent, while the mucosal layer is smooth and devoid of folds. The epithelium lining the whole internal surface of the stomach consists of several types of cells, the most prominent being flattened respiratory epithelial cells. There are also two types of gastric gland cells, three types of endocrine cells (EC), and basal cells. The epithelial layer is underlain by capillaries of a diameter ranging from 6.1-13.1 microm. Capillaries are more numerous in the anterior part of the stomach, where the mean number of capillary sections per 100 microm of epithelium length is 4, compared with 3 in the posterior part. The cytoplasm of the epithelial cells, apart from its typical organelles, contains electron-dense and lamellar bodies at different stages of maturation, which form the sites of accumulation of surfactant. Small, electron-dense vesicles containing acidic mucopolysaccharides are found in the apical parts of some respiratory epithelial cells. Numerous gastric glands (2 glands per 100 microm of epithelium length), composed of two types of pyramidal cells, extend from the surface epithelium into the subjacent lamina propria. The gland outlets, as well as the apical cytoplasm of the cells are Alcian blue-positive, indicating the presence of acidic mucopolysaccharides. Zymogen granules have not been found, but the apical parts of cells contain vesicles of variable electron density. The cytoplasm of the gastric gland cells also contains numerous electron-dense and lamellar bodies. Gastric gland cells with electron-dense cytoplasm and tubulovesicular system are probably involved in the production of hydrochloric acid. Fixation with tannic acid as well as with ruthenium red revealed a thin layer of phospholipids and glycosaminoglycans covering the entire inner surface of the stomach. In regions of the epithelium where the capillaries are covered by the thin cytoplasmic sheets of the respiratory epithelial cells, a thin air-blood barrier (0.25-2.02 microm) is formed, thus enabling gaseous exchange. Relatively numerous pores closed by diaphragms are seen in the endothelium lining the apical and lateral parts of the capillaries. Between gastric gland cells, solitary, noninnervated endocrine cells (EC) of three types were found. EC are characterized by lighter cytoplasm than the surrounding cells and they contain dense core vesicles (DCV) with a halo between the electron-dense core and the limiting membrane. EC of type I are the most abundant. They are of an open type, reaching the stomach lumen. The round DCV of this type, with a diameter from 92-194 nm, have a centrally located core surrounded by a narrow halo. EC of type II are rarely observed and are of a closed type. They possess two kinds of DCV with a very narrow halo. The majority of them are round, with a diameter ranging from 88-177 nm, whil...

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“…Conventional histochemical techniques (PAS, alcian blue, bromophenol blue) are powerful and reliable tools to investigate the characteristics and distribution of carbohydrates and proteins in the digestive system of fishes (Elbal and Agulleiro, 1986;Domeneghini et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

Morpho-histochemical changes in the liver and intestine of young giltheads (fish-nursery), Sparus aurata, L., induced by acute action of the anionic tensioactive alkylbenzene sulphonate

Rosety

Ribelles-García

et al. 2009

Eur J Histochem

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SUMMARYIn the present study we have assessed the effect on the survival and the morpho-histochemical changes in the liver and intestine of young giltheads (fishnursery), Sparus aurata, L., induced by acute action of the anionic tensioactive, alkyl benzene sulphonate (ABS). Firstly, the LC50 of ABS at 96 hours was found to be 0.6 mg/L. Secondly, lots with 50 young giltheads (fish-nursery) were exposed to ABS concentrations of 0.5, 1, 3 and 5 mg/L, to obtain the surface tension value and exposure time required for 50% mortality of the specimens at each tested concentration. Exposure to ABS caused several forms of histopathological damage in the liver (the radial arrangement of hepatocytes was lost) and intestine (destruction of the structure of villi and increase in thickness of the other three layers). In addition, changes in bio-macromolecule components (proteins in general, siderophile proteins, neutral mucopolysaccharides, glycogen and acid mucopolysaccharides) were observed. The degree of these alterations was dependent upon the ABS concentration. These changes could have detrimental effects on the growth and survival of the species. 259 INTRODUCTIONMarine pollution is intimately related to socioindustrial development (Prat and Giraud, 1964;Swedmark et al., 1971). Tensioactives are significant components of several consumer products, such as laundry detergents, shampoos, toothpastes and cosmetics. About 70% of industrial production of tensioactives corresponds to anionic tensioactives (García Dominguez, 1986), the group to which ABS (alkylbenzene sulphonate) belongs.Little is known about the damaging effect of detergents on marine fauna (Mahajan and Singh, 1972;Abel, 1974), in particular about histopathological and histochemical aspects. The aim of this work was firstly to determine the LC50 at 96 h and secondly to estimate the surface tension value and exposure time required for 50% mortality of the specimens resulting at each concentration of ABS. Finally, to examine histopathological alterations as well as the histochemical distribution of proteins (proteins in general, siderophile proteins) and carbohydrates (neutral mucopolysaccharides, glycogen and acid mucopolysaccharides) in two Morpho-histochemical changes in the liver and intestine of young giltheads (fish-nursery), Sparus aurata, L., induced by acute action of the anionic tensioactive alkylbenzene sulphonate

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An immunocytochemical and ultrastructural study of endocrine cells in the gut of a teleost fish, Sparus auratus L

Cited by 54 publications

References 46 publications

Characterization of glycoconjugates in the intestinal mucosa of vertebrates by means of lectin histochemistry.

Characterization of glycoconjugates in the intestinal mucosa of vertebrates by means of lectin histochemistry.

Morphology of the air‐breathing stomach of the catfish Hypostomus plecostomus

Morpho-histochemical changes in the liver and intestine of young giltheads (fish-nursery), Sparus aurata, L., induced by acute action of the anionic tensioactive alkylbenzene sulphonate

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