An immunoassay for pyrethroids: detection of permethrin in meat

Stanker, Larry H.; Bigbee, Carolyn; Emon, Jeanette Van; Watkins, Bruce E.; Jensen, Ronald H.; Morris, Charles; Vanderlaan, Martin

doi:10.1021/jf00087a057

Cited by 74 publications

(52 citation statements)

References 11 publications

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“…In both cases, signal gradually decreased as salt concentration increased, in agreement with the results reported above on the influence of ionic strength on the maximum absorbance, but absorbance dropped more rapidly when the buffer contained Tween 20 than when it did not. An effect of the Tween 20 concentration on the maximum absorbance and sensitivity was also found in immunoassays for pyrethroids and polychlorinated biphenyls (Stanker et al, 1989;Chiu et al, 1995). Concerning carbaryl I 50 , it decreased as salt concentration increased, both in the presence and in the absence of Tween 20 (Figure 4b).…”

Section: Assay Optimization Reagent Concentrations Andmentioning

confidence: 55%

Development of an Enzyme-Linked Immunosorbent Assay to Carbaryl. 2. Assay Optimization and Application to the Analysis of Water Samples

Abad

Montoya

1997

J. Agric. Food Chem.

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The effect of several physicochemical factors on the analytical characteristics of an immunoassay to carbaryl has been studied and this information used to optimize the assay. The immunoassay is based on the LIB-CNH36 monoclonal antibody (MAb) and employs a heterologous coating conjugate. The optimized enzyme-linked immunosorbent assay (ELISA), performed in a high salt concentration buffer with bovine serum albumin instead of Tween 20, has a carbaryl I 50 of 0.058 ppb and a detection limit of 0.010 ppb, which means a 4-fold improvement in the assay sensitivity with respect to the nonoptimized conditions. The assay proved to be very specific for carbaryl since not even 1-naphthol, the main carbaryl metabolite, was recognized. Water samples with very different conductivities were spiked with carbaryl at 0.05, 0.1, and 0.5 ppb and directly analyzed by the developed ELISA. The mean recovery was 112%, and the mean coefficient of variation was 10.7%, which proves the suitability of the method to determine carbaryl in waters at such low levels.

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Section: Assay Optimization Reagent Concentrations Andmentioning

confidence: 55%

Development of an Enzyme-Linked Immunosorbent Assay to Carbaryl. 2. Assay Optimization and Application to the Analysis of Water Samples

Abad

Montoya

1997

J. Agric. Food Chem.

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“…Fused cells were resuspended in Iscove's modified Dulbecco's medium containing, 2 mM-glutamine, 36mM-NaHCO 2 , 100 U ml" 1 of penicillin, lOO/jgmP ' of streptomycin, 5% fetal bovine serum, lOOmM-hypoxanthine, 0.2 mM-aminopterin and 8 mM-thymidine, and pipetted into the wells of 96-well plates containing a feeder layer of mouse macrophages. After 10-14 days, the supernatants were analyzed for antibodies against FBi by a modification of the indirect binding ELISA (idb-ELISA) described by Stanker et al (1989). Ninety-six well flat-bottomed microtiter plates were coated with 100 pi of FBi-BSA (200 ng/well) in water and dried for 24 h at 37°C in a food dehydrator.…”

Section: Monoclonal Antibody Productionmentioning

confidence: 99%

Development of an improved monoclonal antibody‐based Elisa for fumonisin b_1–3and the use of molecular modeling to explain observed detection limits

Elissalde

Kamps‐Holtzapple

Beier

et al. 1995

Food and Agricultural Immunology

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1995) Development of an improved monoclonal antibody-based Elisa for fumonisin b 1-3 and the use of molecular modeling to explain observed detection limits, Food and Agricultural Immunology, 7:2, 109-122, Monoclonal antibodies were prepared against the fumonisins, a group of mycotoxins produced by the plant pathogen, Fusarium moniliforme. Splenic lymphocytes, from Balb/c mice immunized with fumonisin B 1 -ovalbumin conjugate, were fused with SP2/O myeloma cells, and 14 hybridomas were selected. In a competitive enzyme-linked immunosorbent assay, fumonisin B 1 -bovine serum albumin and free fumonisin B 1 (FB 1 ) competed for the monoclonal antibody. The concentrations of FB 1 required to inhibit 50% antibody binding (IC 50 ) ranged from 300 to 670 ppb. Antibodies also cross-reacted with fumonisins B 2 and B 3 (FB 2 , FB 3 ), and the hydrolyzed backbone of fumonisin B 1 (HB-FB 1 ). None of the 14 monoclonal antibodies recognized the sphingolipids, sphingosine and sphinganine, that are structurally similar to the backbone of the fumonisins. Three-dimensional computer models of FB 1 , FB 2 and FB 3 show the amine backbone folding with the two esterified trimethylpropane-1,2,3-tricarboxylic acid side-chains to form a cage into which the hydroxyl and acid groups of these fumonisins extend. The HB-FB 1 molecule, with the two trimethylpropane-1,2,3-tricarboxylic acid esterified moieties at carbons 14 and 15 removed, does not possess two of the three branches which are folded together with inter-hydrogen bonding to formulate the three-dimensional structure that makes up the cage feature of FB 1 , FB 2 and FB 3 . Because of the unexpected folding of the three arms to make a cage of FB 1 , attachment of the protein to the amine group, which is close to, or appears to be pari of, the epitope, may have allowed the immune system of the mouse to produce antibodies more specific for the FB 1 -protein conjugate than to free FB 1 .

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“…Although ELISA has been used successfully in simple ®eld methods for the determination of PCBs in water (Lawruk et al, 1996;Harrison and DeBaise, 1994) and soils (Harrison and Melnychuk, 1995;Lawruk et al, 1996), a logical extension of the ELISA method is to adapt it for determination of PCB concentrations in ®sh tissue extracts. Others have developed sensitive and selective ELISAs for hydrophobic contaminants, such as, chlorinated cyclodienes (Stanker et al, 1991), polychlorinated dibenzo-p-dioxins (Sherry et al, 1989), and permethrin (Stanker et al, 1989) in biological tissue extracts, but in each case the success of the method was dependent on extensive sample preparation prior to the ELISA measurements. In all instances, the ELISA determinative steps retained their inherent bene®ts of simplicity, low cost, and speed.…”

Section: Introductionmentioning

confidence: 99%

Comparison of an enzyme-linked immunosorbent assay (ELISA) to gas chromatography (GC) – measurement of polychlorinated biphenyls (PCBs) in selected US fish extracts

et al. 2000

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Robert O., "Comparison of an enzymelinked immunosorbent assay (ELISA) to gas chromatography (GC) -measurement of polychlorinated biphenyls (PCBs) in selected US fish extracts" (1999). USGS Comparison of an enzyme-linked immunosorbent assay (ELISA) to gas chromatography (GC) ± measurement of polychlorinated biphenyls (PCBs) AbstractThe analysis of PCBs in ®sh tissues by immunoassay methods was evaluated using ®sh collected from a US monitoring program, the National Contaminant Biomonitoring Program of the US Department of Interior, Fish and Wildlife Service. Selected composite whole ®sh samples, which represented widely varying concentrations and sources of PCBs, were extracted and subjected to congener PCB analysis by gas chromatography (GC) and total PCB analysis using an ELISA (ePCBs) calibrated against technical Aroclor 1248. PCB congener patterns in these ®shes were di erent from the patterns found in commercial Aroclors or their combinations as demonstrated by principal component analysis of normalized GC congener data. The sum of the PCB congeners measured by GC (total-PCBs) ranged from 37 to 4600 ng/g (wet weight). Concentrations of PCBs as determined by the ELISA method were positively correlated with total-PCBs and the ePCBs/total-PCBs ratios for individual samples ranged from 1 to 6. Ratios of ePCBs/totalPCBs for dilutions of Aroclors 1242, 1254, and 1260 and for matrix spikes range from 0.6 for 1242 to 2.5 for 1254 and 1260. These results suggest that higher chlorinated PCB congeners have higher a nity for the anti-PCB antibodies. Partial least squares with latent variable analysis of GC and ELISA data of selected Aroclors and ®sh samples also support the conclusion that ELISA derived PCB concentrations are dependent on the degree of chlorination. Ó

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An immunoassay for pyrethroids: detection of permethrin in meat

Cited by 74 publications

References 11 publications

Development of an Enzyme-Linked Immunosorbent Assay to Carbaryl. 2. Assay Optimization and Application to the Analysis of Water Samples

Development of an Enzyme-Linked Immunosorbent Assay to Carbaryl. 2. Assay Optimization and Application to the Analysis of Water Samples

Development of an improved monoclonal antibody‐based Elisa for fumonisin b_1–3and the use of molecular modeling to explain observed detection limits

Comparison of an enzyme-linked immunosorbent assay (ELISA) to gas chromatography (GC) – measurement of polychlorinated biphenyls (PCBs) in selected US fish extracts

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An immunoassay for pyrethroids: detection of permethrin in meat

Cited by 74 publications

References 11 publications

Development of an Enzyme-Linked Immunosorbent Assay to Carbaryl. 2. Assay Optimization and Application to the Analysis of Water Samples

Development of an Enzyme-Linked Immunosorbent Assay to Carbaryl. 2. Assay Optimization and Application to the Analysis of Water Samples

Development of an improved monoclonal antibody‐based Elisa for fumonisin b1–3and the use of molecular modeling to explain observed detection limits

Comparison of an enzyme-linked immunosorbent assay (ELISA) to gas chromatography (GC) – measurement of polychlorinated biphenyls (PCBs) in selected US fish extracts

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Development of an improved monoclonal antibody‐based Elisa for fumonisin b_1–3and the use of molecular modeling to explain observed detection limits