An immunoassay for ochratoxin A without the mycotoxin

Development of a colloidal gold strip for rapid detection of ochratoxin A with mimotope peptide

“…OTA-BSA conjugates were prepared by covalently attaching the carboxylic acid of OTA to BSA (Kawamura et al, 1989). The amino acid residue sequence of mimotope peptide of OTA that was produced in our laboratory was IRPMVDP (Liu, Yu, He, & Xu, 2007). Hydrogen tetrachloroaurate trihydrate was obtained from Aldrich (Milwaukee, WI, USA).…”

Phage-displayed peptides that mimic zearalenone and its application in immunoassay

“…The next procedure of selection of phages by panning-elution was described by Liu et al (2007). After each round of panning-elution selection and amplification, the number of eluted and amplified phages were determined by titering.…”

“…In a search for this alternative, an attempt was made to obtain peptides that mimic ZEN by selection from phage displayed random peptide libraries. Random peptide libraries displayed on phage have been shown to be powerful tools for identifying the peptide and non-peptide epitopes recognised by monoclonal antibodies (McAbs) (Liu, Yu, He, & Xu, 2007;Smith, 1991;Thirumala-Devi, Miller, Reddy, Reddy, & Mayo, 2001;Yuan et al, 1999). In this paper, we describe heptapeptide mimotopes for ZEN that were selected from a phage displayed random peptide libraries and the use of these mimotopes in an analysis of ZEN by ELISA.…”

Surface plasmon resonance biosensor for the detection of ochratoxin A in cereals and beverages

“…Such sample treatment not only makes the analysis time-consuming and costly but also requires skilled personnel. Immunological methods such as enzyme-linked immunosorbent assays (ELISA) have been developed to overcome these limitations, and now are widely used for screening purposes [17][18][19]. Conventional ELISA methods are less demanding for sample clean-up and allow parallel analysis of multiple samples, but they still require several hours to obtain results.…”