2018
DOI: 10.1002/dta.2554
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An immuno polymerase chain reaction screen for the detection of CJC‐1295 and other growth‐hormone‐releasing hormone analogs in equine plasma

Abstract: CJC‐1295 is a 30 amino acid peptide‐based drug that stimulates the release of growth hormone (GH) from the pituitary gland. It is unique among performance‐enhancing peptides due to the presence of a reactive maleimidopropionic acid group that covalently links the peptide to free thiols on the surface of plasma proteins. Once conjugated, CJC‐1295 remains active in the bloodstream for significantly longer than non‐conjugated peptide‐based drugs that are rapidly excreted. Conjugation of CJC‐1295 to plasma protein… Show more

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Cited by 4 publications
(21 citation statements)
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References 23 publications
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“…The vial was incubated for 4 hours at 25°C after which the sample was transferred to a 1.5 mL microfuge tube and centrifuged for 10 minutes at 17 000 x g. The sample was then divided into four equal aliquots and desalted with 0.5 mL zeba desalting columns equilibrated in 1 x PBS according to the manufacturer's instructions. The concentration of CJC‐1295 conjugate in the sample was determined by IPCR testing …”
Section: Methodsmentioning
confidence: 99%
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“…The vial was incubated for 4 hours at 25°C after which the sample was transferred to a 1.5 mL microfuge tube and centrifuged for 10 minutes at 17 000 x g. The sample was then divided into four equal aliquots and desalted with 0.5 mL zeba desalting columns equilibrated in 1 x PBS according to the manufacturer's instructions. The concentration of CJC‐1295 conjugate in the sample was determined by IPCR testing …”
Section: Methodsmentioning
confidence: 99%
“…Plasma was prepared from whole blood samples by spinning vials at 3000 x g for 15 minutes and decanting the clear liquid above the cell pellet. For the administration study, a 10 mg injection of CJC‐1295 was delivered by intramuscular injection to a Thoroughbred gelding as described previously . Whole blood was collected into heparinized vacuum tubes, immediately centrifuged and the plasma transferred to fresh polypropylene tubes before being immediately frozen.…”
Section: Methodsmentioning
confidence: 99%
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