An Imaging Spectroscopy Approach for Measurement of Oxygen Saturation and Hematocrit During Intravital Microscopy

Styp-Rekowska, Beata; Disassa, Nigussie Mecha; Reglin, Bettina; Ulm, Lena; Kuppe, Hermann; Secomb, Timothy W.; Pries, Axel R.

doi:10.1080/10739680601139302

Cited by 30 publications

(20 citation statements)

References 33 publications

(50 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Besides dorsal skinfold windows, spectral imaging of microvasculature has been applied to brain windows 37 and can potentially be applied to other windows, such as mammary windows, 73 or situations where microvasculature can be directly imaged, such as exteriorized mesentery tissue. 32 Another limitation of spectral imaging is that it is not well suited for imaging very rapid dynamic events. The area to be imaged must be relatively stable during the spectral imaging data acquisition time as most spectral imaging systems and instruments require spatial, spectral, or temporal scanning to acquire a complete data set.…”

Section: Discussionmentioning

confidence: 99%

“…Depending on instrument capabilities and the animal model used, correlations in hemoglobin saturation can be obtained throughout a microvessel network with high spatial and temporal resolution. Several researchers have used spectral imaging in preclinical models to measure microvascular hemoglobin saturation in mesentery tissue, 32 cremaster muscle, 33 rodent dorsal skin-fold window chambers, [32][33][34][35][36] and cerebrum. 19,37 In this paper, we highlight novel observations made with our intravital microscopy spectral imaging system used with mouse dorsal skin-fold window chambers for imaging hemoglobin saturation in microvessel networks.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Spectral imaging reveals microvessel physiology and function from anastomoses to thromboses

et al. 2010

View full text Add to dashboard Cite

Abstract. Abnormal microvascular physiology and function is common in many diseases. Numerous pathologies include hypervascularity, aberrant angiogenesis, or abnormal vascular remodeling among the characteristic features of the disease, and quantitative imaging and measurement of microvessel function can be important to increase understanding of these diseases. Several optical techniques are useful for direct imaging of microvascular function. Spectral imaging is one such technique that can be used to assess microvascular oxygen transport function with high spatial and temporal resolution in microvessel networks through measurements of hemoglobin saturation. We highlight novel observation made with our intravital microscopy spectral imaging system employed with mouse dorsal skin-fold window chambers for imaging hemoglobin saturation in microvessel networks. Specifically, we image acute oxygenation fluctuations in a tumor microvessel network, the development of arteriovenous malformations in a mouse model of hereditary hemorrhagic telangiectasia, and the formation of spontaneous and induced microvascular thromboses and occlusions.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Spectral imaging reveals microvessel physiology and function from anastomoses to thromboses

et al. 2010

View full text Add to dashboard Cite

show abstract

“…Measurements of the transmitted or reflected light can then be related to the wavelength-dependent absorption coefficient to yield hemoglobin saturation (200,205,207).…”

Section: Non-invasive Hypoxia Imagingmentioning

confidence: 99%

Imaging Tumor Hypoxia to Advance Radiation Oncology

Lee

Boss

Dewhirst

2014

Antioxidants & Redox Signaling

View full text Add to dashboard Cite

Significance: Most solid tumors contain regions of low oxygenation or hypoxia. Tumor hypoxia has been associated with a poor clinical outcome and plays a critical role in tumor radioresistance. Recent Advances: Two main types of hypoxia exist in the tumor microenvironment: chronic and cycling hypoxia. Chronic hypoxia results from the limited diffusion distance of oxygen, and cycling hypoxia primarily results from the variation in microvessel red blood cell flux and temporary disturbances in perfusion. Chronic hypoxia may cause either tumor progression or regressive effects depending on the tumor model. However, there is a general trend toward the development of a more aggressive phenotype after cycling hypoxia. With advanced hypoxia imaging techniques, spatiotemporal characteristics of tumor hypoxia and the changes to the tumor microenvironment can be analyzed. Critical Issues: In this review, we focus on the biological and clinical consequences of chronic and cycling hypoxia on radiation treatment. We also discuss the advanced non-invasive imaging techniques that have been developed to detect and monitor tumor hypoxia in preclinical and clinical studies. Future Directions: A better understanding of the mechanisms of tumor hypoxia with non-invasive imaging will provide a basis for improved radiation therapeutic practices. Antioxid. Redox Signal. 21, 313-337.

show abstract

“…Shear rate values were estimated by dividing the flow velocity by vessel diameter (d). For determination of oxygen saturation, a multispectral approach was used (Styp-Rekowska et al, 2007).…”

Section: In Vivo Microscopy Time-lapse Imaging and Measurement Of Rementioning

confidence: 99%

Pulsatile shear and Gja5 modulate arterial identity and remodeling events during flow-driven arteriogenesis

et al. 2010

View full text Add to dashboard Cite

SUMMARYIn the developing chicken embryo yolk sac vasculature, the expression of arterial identity genes requires arterial hemodynamic conditions. We hypothesize that arterial flow must provide a unique signal that is relevant for supporting arterial identity gene expression and is absent in veins. We analyzed factors related to flow, pressure and oxygenation in the chicken embryo vitelline vasculature in vivo. The best discrimination between arteries and veins was obtained by calculating the maximal pulsatile increase in shear rate relative to the time-averaged shear rate in the same vessel: the relative pulse slope index (RPSI). RPSI was significantly higher in arteries than veins. Arterial endothelial cells exposed to pulsatile shear in vitro augmented arterial marker expression as compared with exposure to constant shear. The expression of Gja5 correlated with arterial flow patterns: the redistribution of arterial flow provoked by vitelline artery ligation resulted in flow-driven collateral arterial network formation and was associated with increased expression of Gja5. In situ hybridization in normal and ligation embryos confirmed that Gja5 expression is confined to arteries and regulated by flow. In mice, Gja5 (connexin 40) was also expressed in arteries. In the adult, increased flow drives arteriogenesis and the formation of collateral arterial networks in peripheral occlusive diseases. Genetic ablation of Gja5 function in mice resulted in reduced arteriogenesis in two occlusion models. We conclude that pulsatile shear patterns may be central for supporting arterial identity, and that arterial Gja5 expression plays a functional role in flow-driven arteriogenesis.

show abstract

An Imaging Spectroscopy Approach for Measurement of Oxygen Saturation and Hematocrit During Intravital Microscopy

Abstract: The present imaging spectroscopy approach allows detailed assessment of oxygen transport and other functional parameters at the microcirculatory level.

Cited by 30 publications

References 33 publications

Spectral imaging reveals microvessel physiology and function from anastomoses to thromboses

Spectral imaging reveals microvessel physiology and function from anastomoses to thromboses

Imaging Tumor Hypoxia to Advance Radiation Oncology

Pulsatile shear and Gja5 modulate arterial identity and remodeling events during flow-driven arteriogenesis

Contact Info

Product

Resources

About