An image analysis study on nuclear morphology in metastasized and non-metastasized squamous cell carcinomas of the tongue

Hannen, E.J.M.; Laak, J.A.W.M. van der; Manni, Johannes J.; Pahlplatz, M. M. M.; Freihofer, Hans Peter M.; Slootweg, Piet J.; Koole, R.; Wilde, P.C.M. de

doi:10.1002/(sici)1096-9896(199806)185:2<175::aid-path69>3.0.co;2-u

Cited by 16 publications

(16 citation statements)

References 21 publications

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“…In 8226-Dox40 cells, significant variations were observed in E, and ENT, two features related to the spatial organization and distribution of the chromatin (15), and whose values are correlated neither to nuclear area nor to global chromatin condensation. Significant changes in chromatin higher-order organisation, evaluated through nuclear texture, have already been reported as markers of tumour cell aggressiveness or metastatic potential, either in experimental or clinical samples (22)(23)(24)(25). For instance, LM displays significant increases in invasive bladder tumours as compared to superficial tumours (25) and appears linked (together with E and GLD) to tumour size in breast carcinomas (unpublished data).…”

Section: Discussionmentioning

confidence: 80%

Thalidomide alters nuclear architecture without ABCB1 gene modulation in drug-resistant myeloma cells

Trussardi-Regnier¹

2009

Int J Oncol

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Abstract. ABCB1 gene overexpression has been described as an important mechanism for resistance to conventional chemotherapy in multiple myelomas. In the refractory multiple myelomas, other drug regimens have been successfully applied, including thalidomide treatments. Besides its well-documented anti-angiogenic effects, thalidomide therapy could result in a decrease in ABCB1 gene expression. In this study, we analysed the effects of a 24-h short-term treatment by thalidomide or its active metabolite phthaloyl glutamic acid (PGA) on nuclear chromatin higher-order organisation and ABCB1 gene expression in drug-sensitive and drug-resistant 8226 human myeloma cells. As compared to sensitive cells, 8226-Dox40 drug-resistant cells exhibited an increase in chromatin texture condensation and ABCB1 gene overexpression. At this gene promoter level, the -50 and -100 GC boxes displayed an unmethylated profile in drug-sensitive cells whereas drug-resistant cell promoter GC boxes were fully methylated. Thalidomide and PGA induced significant chromatin textural changes in 8226-Dox40 drug-resistant cells only with neither alteration in ABCB1 gene expression nor methylation profile of its promoter. Conversely thalidomide and PGA induced down-regulation of VEGF gene expression in both drug-sensitive and -resistant myeloma cells. These data suggest that short-term treatments by thalidomide or PGA do not induce any significant change on ABCB1 gene expression though they modulate chromatin supra-organisation in drug-resistant 8226 human myeloma cells.

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Section: Discussionmentioning

confidence: 80%

Thalidomide alters nuclear architecture without ABCB1 gene modulation in drug-resistant myeloma cells

Trussardi-Regnier¹

2009

Int J Oncol

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“…Similar increases in chromatin condensation, evaluated through nuclear texture, have already been reported as markers of tumor cells aggressiveness or metastatic potential, either in experimental or clinical samples. [27][28][29][30] For instance, LM displayed significant increases in invasive bladder tumors as compared to superficial tumors. 30 In RPMI 8226d cells, these textural changes were associated with a lower DNase I sensitivity, indicating a decreased accessibility linked to a more compact chromatin conformation.…”

Section: Discussionmentioning

confidence: 99%

Nuclear chromatin patterns in 3 glucocorticoid-resistant RPMI 8226 human myeloma cell sub-lines: Correlations with cell growth and immunological phenotype

Genty

El-Khoury

Liautaud-Roger

et al. 2005

Cancer Biology & Therapy

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“…25 Approximately 200 nonoverlapping, well demarcated, not mechanically damaged and nonpyknotic inter- …”

Section: Nuclear Morphologymentioning

confidence: 99%

“…23,24 Feulgen-stained nuclei in tongue carcinomas have been assessed to predict metastasis. 25 Computerized features regarding the chromatin condensation in the nuclei and the roundness of nuclei showed good association with metastasis and identified metastasized tumors correctly in 72% of 70 cases. 25 These results indicate that, although other factors than DNA composition influence nuclear morphology, it appears helpful in predicting metastasis in patients with tongue carcinoma.…”

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confidence: 91%

“…25 Computerized features regarding the chromatin condensation in the nuclei and the roundness of nuclei showed good association with metastasis and identified metastasized tumors correctly in 72% of 70 cases. 25 These results indicate that, although other factors than DNA composition influence nuclear morphology, it appears helpful in predicting metastasis in patients with tongue carcinoma. Genetic damage associated with neoplastic conversion such as disabling the p53 tumor suppressor gene also is associated with angiogenesis, i.e., tumor-induced formation of new vessels.…”

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confidence: 94%

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Improved prediction of metastasis in tongue carcinomas, combining vascular and nuclear tumor parameters

Hannen

Laak

Manni

et al. 2001

Cancer

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A new series of photocleavable protein cross‐linking reagents based on bis(maleimide) derivatives of diaryl disulfides have been synthesised. They have been functionalised with cysteine and transient absorption spectra for the photolysis reaction have been recorded by using the pump‐probe technique with a time resolution of 100 femtoseconds. Photolysis of the disulfide bond yields the corresponding thiyl radicals in less than a picosecond. There is a significant amount of geminate recombination, but some of the radicals escape the solvent cage and the quantum yield for photocleavage is 30 % in water.

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An image analysis study on nuclear morphology in metastasized and non-metastasized squamous cell carcinomas of the tongue

Cited by 16 publications

References 21 publications

Thalidomide alters nuclear architecture without ABCB1 gene modulation in drug-resistant myeloma cells

Thalidomide alters nuclear architecture without ABCB1 gene modulation in drug-resistant myeloma cells

Nuclear chromatin patterns in 3 glucocorticoid-resistant RPMI 8226 human myeloma cell sub-lines: Correlations with cell growth and immunological phenotype

Improved prediction of metastasis in tongue carcinomas, combining vascular and nuclear tumor parameters

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