An idiotypic-anti-idiotypic competitive immunoassay for quantitation of okadaic acid

Shestowsky, William S.; Quilliam, Michael A.; Sikorska, Hanna

doi:10.1016/0041-0101(92)90519-b

Cited by 42 publications

(13 citation statements)

References 14 publications

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“…Such properties are similar to the recent observation for the anti-id antibodies generated in rabbits after immunizing with a monoclonal antibody against anti-AFB3-HS-BSA (Hsu & Chu, 1994). These observations are also similar to data obtained from studies for the anti-id antibodies of several other hapten-type toxins and insecticides (Chanh et al, 1990;Shestowsky et al, 1992;Spinks et al, 1993).…”

Section: Discussionsupporting

confidence: 89%

“…Within the last few years, numerous anti-id antibodies against small molecular weight haptens, including mycotoxins such as T-2 toxin (Chanh et al, 1989(Chanh et al, , 1990(Chanh et al, , 1992, insecticides, herbicides (Spinks et al, 1993), hormones (Khole & Hegde, 1992;Madhok et al, 1992) and phycotoxins (Shestowsky et al, 1992(Shestowsky et al, , 1993 have been successfully generated. Some anti-id polyclonal antibodies (Ab2) have been shown to mimic effectively the biological functions of the hapten (Nisonoff, 1991).…”

Section: Introductionmentioning

confidence: 99%

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Anti‐idiotype and anti‐anti‐idiotype antibodies generated from polyclonal antibodies against aflatoxin b₁

Hsu

Chu

1995

Food and Agricultural Immunology

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Polyclonal anti-idiotype antibodies pAb2) for aflatoxin were generated from mice ascites after immunization with affinity-purified rabbit polyclonal antibody (pAbl) against aflatoxin B 1 (pAFB 1 ). After affinity chromatography purification, pAb2 were subjected to various analyses, and were used to generate anti-anti-id antibodies (pAb3) in the ascites of Balb/c mice. ELISA analyses revealed that the anti-id antibodies bound specifically to the original pAb1, but not to other types of monoclonal antibodies or normal mouse IgG. The inhibition of binding of AFB 1 to pAb1 by pAb2 was demonstrated in both regular and biotin-avidin amplified enzyme-linked immunosorbent assay (ELISA) systems in which AFB 1 -bovine serum albumin (BSA) was coated on to the microtiter plate. The concentrations causing 50% inhibition (ID 50 ) of the binding of pAbl to AFB 1 -BSA by pAb2 were found to be 6.5 and 1.7 µg/assay respectively. Inhibition of the binding of pAb1 to the solid-phase pAb2 by free AFB 1 (ID 50 = 0.63 µ/assay) was also demonstrated in the ELISA. pAb3 were found to have similar characteristics to the original pAb1. In the direct ELISA, the ID 50 of binding of AFB 1 -horseradish peroxidase to the solid-phase pAb3 by AFB 1 was found to be 0.20 ng ml -1 . In the indirect ELISA, the ID 50 of the binding of pAb3 to the solid-phase AFB 1 -BSA by AFB 1 was found to be 1.84 ng ml -1 Analysis of a naturally contaminated corn sample for AFB 1 by the pAb3-based ELISA gave a result similar to that obtained from the regular pAb1-based ELISA (19.7 and 21.8 µg kg -1 respectively).

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Section: Discussionsupporting

confidence: 89%

Section: Introductionmentioning

confidence: 99%

Anti‐idiotype and anti‐anti‐idiotype antibodies generated from polyclonal antibodies against aflatoxin b₁

Hsu

Chu

1995

Food and Agricultural Immunology

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“…This precludes the use of IgY2 as an immunochemical reagent for AFB analysis. Such properties are similar to recent observations for the anti-id antibodies generated in rabbits and mice (Hsu & Chu, 1994, 1995, as well as data obtained from studies for the anti-id antibodies of several other hapten-type toxins and insecticides (Chanh et al, 1990;Shestowsky et al, 1992;Spinks et al, 1993). Whereas IgY2 appear to have little use in the immunoassay of aflatoxins, IgY2 could be used as a good immunogen in generating the Ab3 as in previous studies (Hsu & Chu, 1994, 1995.…”

Section: Production and Characterization Of Egg Yolk Igy3supporting

confidence: 82%

Anti‐idiotype and anti‐anti‐idiotype antibodies for aflatoxin from laying hens

Hsu

Chu

1995

Food and Agricultural Immunology

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Anti-idiotype (anti-id) antibodies (IgY2)for aflatoxin (AF) were obtained from the egg yolks of laying hens immunized with affinity-purified rabbit polyclonal anti-aflatoxin B 1 (AFB 1 ) carboxymethyloxime-bovine serum albumin (BSA) antibodies (pAb1). The IgY2 were affinity purified and then subjected to various analyses. Inhibition of the binding of pAbl to the solid-phase AFB 1 -BSA by IgY2 and the binding of pAb1 to the solid-phase IgY2 by free AFB 1 were demonstrated in a biotin-avidin amplified enzyme-linked immunosorbent assay (ELISA) system. The concentration of IgY2 causing 50% inhibition (ID 50 ) of the binding of pAb1 to AFB 1 -BSA was found to be 2.45 µg/assay. The ID 50 concentration of the binding of pAb1 to IgY2 by free AFB 1 was found to be 0.30 µg/assay. Inhibition of the binding of AFB 1 -horseradish peroxidase (HRP) to the solid-phase pAbl by IgY2 (ID50 = 9.65 µg/assay) was also demonstrated in the direct ELISA. Egg yolk anti-anti-id antibodies (IgY3) were obtained by immunizing laying hens with rabbit pAb2 against anti-AFB 3 -hemisuccinate-BSA monoclonal antibody. IgY3 was subjected to affinity chromatography purification with Sepharose gel armed with AFB 2 -carboxymethytoxime, and then subjected to various analyses. ELISA analysis revealed that IgY3 has characteristics similar to other anti-AFB antibodies induced in various experimental animals. In the direct ELISA, the ID 50 of the binding of AFB 1 -HRP to solid-phase lgY3 by AFB 1 was found to be 0.12 ng ml -1 . In the indirect ELISA, the ID 50 of the binding of IgY3 to solid-phase AFB 1 -BSA by AFB 1 was found to be 2.2 ng ml -1 . The IgY3-based ELISA analysis showed higher sensitivity than that of the egg yolk antibodies directly against AFB-protein conjugates (IgY1). A good correlation was found for the data obtained from IgY3-based and pAb1-based ELISAs in the analysis of AFB in the fungal culture filtrates.

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“…1992, Sheskowsky et al 1992, domoic acid (Newsome et al 1991), saxitoxin (F. S. Chu, University of Wisconsin, personal communication), tetrodotoxin , palytoxin , pectenotoxins (Holmes 1991) and brevetoxins (Baden et al 1992).…”

Section: Monitoring Of Susceptible Products Prior To or During Harvestmentioning

confidence: 98%

Surveillance programmes for managing risks from naturally occurring toxicants

Park

1995

Food Additives and Contaminants

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Aflatoxins are potent carcinogenic, mutagenic and teratogenic metabolites produced by moulds that grow on food and feed. Their toxicity has caused severe health and economic problems worldwide. Other mycotoxins which have been associated with human health risks include ergot alkaloids, citreoviridin, trichothecenes, ochratoxins, citrinin, tremorgenic mycotoxins and the fumonisins. Many phycotoxins have also been associated with human illnesses; these phycotoxins include paralytic shellfish poisons, diarrhoeic shellfish poisons, ciguatera-related toxins, neurotoxic shellfish poisons, tetrodotoxin and, the more recently discovered, domoic acid which is associated with amnesic shellfish poisoning. Human exposure to these naturally occurring toxins can be from direct consumption of contaminated commodities or from foods derived from animals previously exposed to these toxins in their feed. Food safety monitoring programmes for selected toxins have been established for raw and finished products susceptible to contamination. Components of these control programme include the establishment of regulatory limits or guidelines, monitoring susceptible products for specific compounds, and the diversion of the contaminated products to lower-risk uses and/or decontamination procedures.

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An idiotypic-anti-idiotypic competitive immunoassay for quantitation of okadaic acid

Cited by 42 publications

References 14 publications

Anti‐idiotype and anti‐anti‐idiotype antibodies generated from polyclonal antibodies against aflatoxin b₁

Anti‐idiotype and anti‐anti‐idiotype antibodies generated from polyclonal antibodies against aflatoxin b₁

Anti‐idiotype and anti‐anti‐idiotype antibodies for aflatoxin from laying hens

Surveillance programmes for managing risks from naturally occurring toxicants

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An idiotypic-anti-idiotypic competitive immunoassay for quantitation of okadaic acid

Cited by 42 publications

References 14 publications

Anti‐idiotype and anti‐anti‐idiotype antibodies generated from polyclonal antibodies against aflatoxin b1

Anti‐idiotype and anti‐anti‐idiotype antibodies generated from polyclonal antibodies against aflatoxin b1

Anti‐idiotype and anti‐anti‐idiotype antibodies for aflatoxin from laying hens

Surveillance programmes for managing risks from naturally occurring toxicants

Contact Info

Product

Resources

About

Anti‐idiotype and anti‐anti‐idiotype antibodies generated from polyclonal antibodies against aflatoxin b₁

Anti‐idiotype and anti‐anti‐idiotype antibodies generated from polyclonal antibodies against aflatoxin b₁