An Icam‐1 Like Cell Adhesion Molecule Is Responsible for Cd34 Positive Haemopoietic Stem Cells Adhesion to Bone‐marrow Stroma

Rao, Seema Rajesh; Chitnis, Vivek; Deora, Anupama; Tanavde, Vivek; Desai, Salomi

doi:10.1006/cbir.1996.0029

Cited by 12 publications

(7 citation statements)

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“…Epidermal keratinocytes express two major pairs of keratin polypeptides, K5/K14 and K1/K10. K5/K14 is expressed specifically in the basal generative compartment and K1/K10 specifically in the differentiating suprabasal compartment [ 34 ]. Previous reports have shown that psoriatic epidermis contains lower amounts of K10 and higher amounts of K14 and K16 [ 35 , 36 ].…”

Section: Discussionmentioning

confidence: 99%

Analysis of epithelial–mesenchymal transition markers in psoriatic epidermal keratinocytes

Man

Chen

et al. 2015

Open Biol.

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Psoriasis is similar to endpoints of epithelial–mesenchymal transition (EMT), a process of epithelial cells transformed into fibroblast-like cells. The molecular epithelial and mesenchymal markers were analysed in psoriatic keratinocytes. No obvious alteration of epithelial markers E-cadherin (E-cad), keratin 10 (K10), K14 and K16 was detected in psoriatic keratinocytes. However, significantly increased expression of Vim, FN, plasminogen activator inhibitor 1 (PAI-1) and Slug was seen. IL-17A and IL-13 at 50 ng ml−1 strongly decreased expression of K10, Vim and FN. TGF-β1 at 50 ng ml−1 promoted the production of N-cad, Vim, FN and PAI-1. Slug was decreased by dexamethasone (Dex), but E-cad was upregulated by Dex. Silencing of ERK partially increased E-cad and K16, but remarkably inhibited K14, FN, Vim, β-catenin, Slug and α5 integrin. Moreover, inhibition of Rho and GSK3 by their inhibitors Y27632 and SB216763, respectively, strongly raised E-cad, β-catenin and Slug. Dex decreased Y27632-mediated increase of β-catenin. Dex at 2.0 µM inhibited SB216763-regulated E-cad, β-catenin and slug. In conclusion, EMT in psoriatic keratinocytes may be defined as an intermediate phenotype of type 2 EMT. ERK, Rho and GSK3 play active roles in the process of EMT in psoriatic keratinocytes.

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Section: Discussionmentioning

confidence: 99%

Analysis of epithelial–mesenchymal transition markers in psoriatic epidermal keratinocytes

Man

Chen

et al. 2015

Open Biol.

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“…Because proper keratin gene expression and filament organization are absolutely necessary for normal functioning of the skin [26] and integrins are the main way by which cells both bind to and respond to their environment [27], the up-regulation of these classes of proteins could represent a mechanism for maintenance of functional properties of the epidermis.…”

Section: Discussionmentioning

confidence: 99%

Effects of UVB-induced oxidative stress on protein expression and specific protein oxidation in normal human epithelial keratinocytes: a proteomic approach

et al. 2010

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BackgroundThe UVB component of solar ultraviolet irradiation is one of the major risk factors for the development of skin cancer in humans. UVB exposure elicits an increased generation of reactive oxygen species (ROS), which are responsible for oxidative damage to proteins, DNA, RNA and lipids. In order to examine the biological impact of UVB irradiation on skin cells, we used a parallel proteomics approach to analyze the protein expression profile and to identify oxidatively modified proteins in normal human epithelial keratinocytes.ResultsThe expression levels of fifteen proteins - involved in maintaining the cytoskeleton integrity, removal of damaged proteins and heat shock response - were differentially regulated in UVB-exposed cells, indicating that an appropriate response is developed in order to counteract/neutralize the toxic effects of UVB-raised ROS. On the other side, the redox proteomics approach revealed that seven proteins - involved in cellular adhesion, cell-cell interaction and protein folding - were selectively oxidized.ConclusionsDespite a wide and well orchestrated cellular response, a relevant oxidation of specific proteins concomitantly occurs in UVB-irradiated human epithelial Keratinocytes. These modified (i.e. likely dysfunctional) proteins might result in cell homeostasis impairment and therefore eventually promote cellular degeneration, senescence or carcinogenesis.

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“…The lower expression of the adhesion molecule CD54 (ICAM-1) found on the CD34 1 cells in group III might impair interactions between these stem cells and stroma after the first steps of homing, as can be inferred from an earlier study (Rao et al, 1996).…”

Section: Discussionmentioning

confidence: 76%

The phenotypic profile of CD34‐positive peripheral blood stem cells in different mobilization regimens

et al. 2000

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Summary. The type of regimen used might result in mobilization of phenotypically and functionally different CD34 1 cells. We compared the phenotype of CD34 1 cells in leukapheresis products of three homogeneous groups: I, healthy individuals treated with granulocyte colony-stimulating factor (G-CSF) alone (n 13); II, patients mobilized with G-CSF following chemotherapy (n 16); and III, patients mobilized with G-CSF after high-dose chemotherapeutic pretreatment (n 24). Multiparameter flow cytometry was performed for CD341 subpopulation analysis and focused on adhesion molecules, differentiation markers and megakaryocytic markers relevant for stem cell homing, with special reference to the importance of L-selectin expression. Regimens I and II led to higher numbers of mobilized CD34 subpopulations that were also positive for L-selectin, there was no significant difference between the three regimens. A similar approach for the megakaryocytic CD34 1 population resulted in an even worse quality of regimen III: 5´1% of CD34 1 being CD41 1 /L-selectin 1 compared with 9´2% and 8´9% in regimens I and II respectively. We concluded that the phenotypes of the CD34 1 cells in the G-CSF (group I) and G-CSF±chemotherapy (group II) regimens are similar, whereas the phenotype of the CD34 1 cells mobilized in the high-dose regimen (group III) displayed features that might negatively influence homing of the cells. Future studies will be directed towards regimens that will lead to the mobilization of a higher amount of CD34 1 cells with a phenotypically favourable phenotype.

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An Icam‐1 Like Cell Adhesion Molecule Is Responsible for Cd34 Positive Haemopoietic Stem Cells Adhesion to Bone‐marrow Stroma

Cited by 12 publications

References 0 publications

Analysis of epithelial–mesenchymal transition markers in psoriatic epidermal keratinocytes

Analysis of epithelial–mesenchymal transition markers in psoriatic epidermal keratinocytes

Effects of UVB-induced oxidative stress on protein expression and specific protein oxidation in normal human epithelial keratinocytes: a proteomic approach

The phenotypic profile of CD34‐positive peripheral blood stem cells in different mobilization regimens

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