An in vivo study of hydrogels based on physiologically clotted fibrin–gelatin composites as wound‐dressing materials

Abstract

In this study we have reported the efficacy of three biomaterials: (a) physiologically clotted fibrin-gelatin composite (PFG), (b) PFG graft copolymerized with 2-hydroxyethyl methacrylate (PFG-HEMA), and (c) PFG graft copolymerized with 2-hydroxypropyl methacrylate (PFG-HPMA) as temporary wound-dressing materials using the rat as an animal model. Full-thickness excision wounds were made on the back of female rats weighing about 150 +/- 10 g. The dressings were applied on the wounds and changed periodically at an interval of 4 days with the respective materials. The wounds treated with PFG-HEMA healed completely on 15th day after wound creation, whereas those treated with PFG and PFG-HPMA resulted in complete healing on the 17th day. The concentrations of collagen, hexosamine, and uronic acid in the granulation tissue were determined. The PFG and its graft copolymers acted as hydrogels, thereby absorbing excess exudates, while still maintaining a moist environment at the wound site. The enhanced wound healing in the experimental animals was reflected in the increased rate of wound contraction. The results of the histological and mechanical studies of the experimental groups revealed that reepithelialization and remodeling of the skin have been achieved by providing a moist environment at the wound site by the biomaterials and thereby hastening the migration of keratinocytes.

Effect of growth factors and pro-inflammatory cytokines by the collagen biocomposite dressing material containing Macrotyloma uniflorum plant extract—In vivo wound healing

“…Collagen forma- tion and maturation at the wound site play an important role in increasing the tensile strength of the healed skin tissue. Remodeling of collagen takes place during the maturation phase, where the molecules are cross-linked to form fibers due to the formation of inter and intra-molecular cross links, thereby increasing tensile strength [33].…”

Fibrinogen and fibrin based micro and nano scaffolds incorporated with drugs, proteins, cells and genes for therapeutic biomedical applications

“…In one study, Fbn-gelatin composite film was shown to exhibit a high equilibrium content of water, which could effectively improve the efficacy and properties of the matrix when applied as a wound dressing. 82 In other studies, a physiologically coated Fbn-chitosan gelatin composite film was shown to provide a fibrous and porous structure with a high water absorption capacity, which could improve the encapsulation efficacy of hydrophilic protein and peptides. 83 Hepatic cell-seeded Fbggelatin matrix stabilized by thrombin exhibited a very high compressive strength.…”

Polycaprolactone-based fused deposition modeled mesh for delivery of antibacterial agents to infected wounds

“…Photographs of the wound healing patterns were taken for qualitative analysis [28] 2.3.3. Weight measurement Weight of mice were recorded before the surgery with an electronic mass balance (accurate to 0.01 g) and again at time of sacrifice.…”