Anin vivomodel for the study of chemotaxis induced by Schistosomula ofSchistosoma mansoni

Abstract

Peritoneal leucocytosis, with an increased percentage of eosinophils, was found in mice which had been infected with Schistosoma mansoni for 7 weeks or longer. Specific IgG against worm and egg antigens increased in peritoneal fluids and their corresponding sera respectively 5 and 7 weeks after infection. An intraperitoneal challenge with schistosomula elicited neutrophilia in all mice regardless of immune status, as well as infiltration of eosinophils and macrophages in infected mice. The secondary eosinophilia occurred in mice previously infected for 1 week or longer, whereas the infiltration of macrophages occurred only after worms from the primary infection had started laying eggs. Unlike the eosinophilia the macrophage response required infection with bisexual populations of cercariae. Injection of previously infected mice with Escherichia, Trichinella or Toxocara failed to increase the proportions of eosinophils and macrophages. Schistosomula-induced eosinophilia could be elicited in passively sensitized mice. Intraperitoneal injection of PBS extract of adult worms elicited eosinophilia in infected mice and neutrophilia in normal mice. Two chromatographic fractions induced eosinophilia and the third only neutrophilia. The relevance of these observations to host response to S. mansoni infections is discussed.

Phenotypic plasticity of maleSchistosoma mansonifrom the peritoneal cavity and hepatic portal system of laboratory mice and hamsters

“…As an advantage, peritoneal infection allows a simple and rapid method for mass screening of prophylactic agents to combat schistosomiasis (Pereira et al , 1974). This method of study has also helped to elucidate aspects of the early stages and immunology of the host–parasite relationship (Melo et al , 1978, 1980, 1993a, b, 1994; Melo & Pereira, 1980, 1985; Chao et al , 1986). Indeed, it has been demonstrated that parasites recovered from the peritoneal cavity of two albino mouse strains (AKR/J and Swiss) are significantly different in relation to the total body length of the worms, the intraperitoneal worm burden and the maturation degree of the schistosomes (Bicalho et al , 1993).…”

Schistosoma mansoni: inflammatory foci around larvae in the peritoneal cavity of naive mice is radiosensitive

“…Some studies have highlighted the macrophages or peritoneal cells as active role during S. mansoni infection by classical routes 3,11 or the neutrophils as an example of the first line defence in a peritoneal cavity of mice model 15,16 . However, little attention has been done to wholebody irradiation protocols in, which are might expect disable innate peritoneal resistance in mice against S. mansoni compared with other radiation protocols 13 .…”