An in vivo approach showing the chemotactic activity of leukotriene B ₄ in acute renal ischemic-reperfusion injury

Gastrin-releasing peptide receptor (GRPR) mediates chemotaxis in neutrophils

“…Neutrophil migration is governed by gradients of chemoattractants, which include chemokines, complement-derived peptides (26), bacterial peptides (35), and lipid mediators (21). Despite the different nature of the ligands, all of them bind to and activate GPCR (26).…”

“…Chemokines (17) and leukotrienes (21) and molecules released by damaged tissues (22,23) act as chemoattractants, acting directly on neutrophils to induce migration. We investigated whether GRP, a neuropeptide, would induce neutrophils to migrate up a gradient of GRP in vitro, in a Transwell system.…”

A Novel Hepatointestinal Leukotriene B4 Receptor

“…Guinea pig spleen membrane preparations have high affinity for LTB 4 and contain moderate number of LTB 4 binding sites (37); this receptor has high affinity for LTB 4 and 20-hydroxy-LTB 4 but low affinity for 20-carboxy-LTB 4 (37), a profile similar to LTB 4 -R2 (Table I). In a rat renal ischemiareperfusion injury model, Chinese hamster ovary cells expressing a LTB 4 receptor accumulate along with neutrophils in the postischemic kidney; use of LTB 4 antagonists led to the marked decrease in accumulation of Chinese hamster ovary cells and neutrophils (38). Spontaneously hypercholesterolemic rats, characterized by glomerular infiltration of macrophages, fed a normal diet developed end-stage renal failure in 26 weeks, while those fed a diet supplemented with a LTB 4 antagonist showed normal renal function (39).…”

Regulating leukotriene synthesis: The role of nuclear 5‐lipoxygenase

“…For example, elevated LT levels clearly contribute to some forms of asthma [Drazen et al, 1992;Daffern et al, 1999] and pharmaceutical intervention in LT production or signaling reduce symptoms during asthma exacerbations [Dockhorn et al, 2000;Garcia Garcia et al, 2005]. The overproduction of LTs has also been associated with allergic diseases [Talbot et al, 1985;Taylor et al, 1989;Rachelefsky, 1997], pulmonary fibrosis [Wilborn et al, 1996], atherosclerosis [Spanbroek et al, 2003;Dwyer et al, 2004], hyperlipidemiadependent inflammation of the arterial wall [Zhao et al, 2004], pulmonary hypertension [Voelkel et al, 1996], arthritis [Giffiths et al, 1995], and ischemia reperfusion injury [Noiri et al, 2000]. In addition, increased 5-LO expression and presumably increased LT synthesis, has been associated with lung cancer [Avis et al, 2005], pancreatic cancer [Hennig et al, 2002], bladder cancer [Yoshimura et al, 2003], breast cancer [Jiang et al, 2003], colon cancer [Nielsen et al, 2003], glioblastoma multiforme [Golubic et al, 2003], prostate cancer , testicular cancer , and esophageal cancer [Hoque et al, 2005].…”