An<i>In Vitro</i>Study of Differentiation of Hematopoietic Cells to Endothelial Cells

Wang, Qi Ru; Wang, Bao He; Zhu, Wen; Huang, Yan; Li, Yi; Yan, Qi

doi:10.1155/2011/846096

Cited by 15 publications

(11 citation statements)

References 31 publications

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“… 25 In addition, bound VEGF may influence EPC differentiation into mature EC-like phenotypes, while increasing the migration and proliferation of ECs. 26 − 29 Besides its influence on EPCs, diffusion of VEGF may also induce the migration and proliferation of resident ECs from mature vessels across anastomotic sites. 30 , 31 VEGF has been successfully delivered via scaffolds utilizing specific binding motifs present in heparin.…”

Section: Introductionmentioning

confidence: 99%

Contrasting Biofunctionalization Strategies for the Enhanced Endothelialization of Biodegradable Vascular Grafts

Melchiorri

Hibino²,

Yi³

et al. 2015

Biomacromolecules

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Surface modification of biodegradable vascular grafts is an important strategy to improve the in situ endothelialization of tissue engineered vascular grafts (TEVGs) and prevent major complications associated with current synthetic grafts. Important strategies for improving endothelialization include increasing endothelial cell mobilization and increased endothelial cell capture through biofunctionalization of TEVGs. The objective of this study was to assess two biofunctionalization strategies for improving endothelialization of biodegradable polyester vascular grafts. These techniques consisted of cross-linking heparin to graft surfaces to immobilize vascular endothelial growth factor (VEGF) or antibodies against CD34 (anti-CD34Ab). To this end, heparin, VEGF, and anti-CD34Ab attachment and quantification assays confirmed the efficacy of the modification strategy. Cell attachment and proliferation on these groups were compared to unmodified grafts in vitro and in vivo. To assess in vivo graft functionality, the grafts were implanted as inferior vena cava interpositional conduits in mice. Modified vascular grafts displayed increased endothelial cell attachment and activity in vivo, according to microscopy techniques, histological results, and eNOS expression. Inner lumen diameter of the modified grafts was also better maintained than controls. Overall, while both functionalized grafts outperformed the unmodified control, grafts modified with anti-CD34Ab appeared to yield the most improved results compared to VEGF-loaded grafts.

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Section: Introductionmentioning

confidence: 99%

Contrasting Biofunctionalization Strategies for the Enhanced Endothelialization of Biodegradable Vascular Grafts

Melchiorri

Hibino²,

Yi³

et al. 2015

Biomacromolecules

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“…The main reason of IHF development are myocardium infarct, because structural remodeling of a myocardium is associated with inflammation, formation of scar, and development of interstitial fibrosis in peri-infarct zone and remodeling of blood supply of a myocardium [8]. Bone marrow mononuclear cells contain EPCs and MSCs valuable in stem cell therapy to enhanced postischemic neovascularization [9][10][11].…”

Section: Discussionmentioning

confidence: 99%

Phenotype of bone-marrow mononuclear cells before and after short-time precondition with erythropoietin from patients with ischemic heart failure

Лыков¹,

Повещенко²,

Cherniavsky³

et al. 2018

Russ Open Med J

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Aim-To reveal the results of the condition of the bone marrow mononuclear cells (BM-MNCs) with erythropoietin (Epo). Material and Methods-There were 30-ty patients with coronary artery disease (CAD) enrolled in this study during 2016-2017 years. 95% were men. All were in angina NYHA class II-III. Hypertension presented in 90%, peripheral atherosclerosis in 60%. BM-MNCs were obtained by centrifugation of bone marrow aspirate on Ficoll-Paque, washed, and then preconditioned with Epo. Phenotype, cell cycle, cell death, proliferation, migration, and tube formation before, and after precondition BM-MNCs with Epo were done. Results-In this study, we observed the presence in cellular graft of the hematopoietic stem cells (HSCs), and endothelial progenitor cells (EPCs) at the different stage of maturation/differentiation, and mesenchymal stem cells (MSCs). Precondition BM-MNCs with Epo increased number of HSCs carrying erythropoietin receptor (EpoR), and EPCs carrying CD184. Also, Epo detained СВ34+ cells in a rest phase of cell cycle (G0G1). Condition media from BM-MNCs treated with Epo augment tube formation and wound healing by EA.hy 929. Conclusion-Epo in vitro increased number of the stem cells carrying EpoR and CD184, and increased accumulation of CD34+ cell in G0G1 phase of cell cycle, and induced production of proangiogenic factor by BM-MNCs. Further investigation is needed to assess the type of cytokines produced by BM-MNCs after condition with Epo.

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“…One of the methods of obtained EPCs from bone marrow without aspiration is G-CSF administration which leads to mobilization of progenitor cells from bone marrow [9]. Previous study showed that EPCs secreted a number of cytokines that could stimulate proliferation, migration, and survival of endothelial cells [10][11][12][13][14]. Endothelial progenitor cells promote vasculogenesis and/or ameliorate the process of angiogenesis, thereby improving both regeneration and function of ischemic organs such as the infracted heart [15].…”

Section: Discussionmentioning

confidence: 99%

Cytokine Production by Circulating Endothelial Progenitor Cells before and after G-CSF Mobilization

Лыков¹,

Poveschenko²,

Бондаренко³

et al. 2016

Stud Stem Cells Res Ther

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Patients and study design Ten patients (7 men and 3 women between 55 and 66 years) with CIHD and no-option for revascularization consecutive patients with chronic myocardial infarction and end-stage chronic heart failure under optimized pharmacological therapy (β-blockers, ACE inhibitors/ATR-blockers, diuretics) were treated with subcutaneous injections of 300 μg per day G-CSF (Grasalva) for 5 days subsequently days. Inclusion criteria comprised: a history of myocardial infarction >12 months before the enrollment and a fixed perfusion defect on Tc-99m tetrofosmin SPECT; the class of clinical symptoms of heart failure; non revascularisable patient who is symptomatic on optimal medical therapy left ventricular ejection fraction <35% as determined by two-dimensional echocardiography were recruited from Novosibirsk Research Institute of Circulation Pathology of EN Meshalkin MH RF. The following exclusion criteria were applied: eligibility for per cutaneous coronary intervention, coronary artery bypass grafting, previous valve surgery, surgical remodeling of the left ventricle or cardiac resynchronization therapy; hemorrhagic symptoms, severe renal and liver dysfunction, and the history of malignancy. Isolation, cultivation, and characterization of endothelial cells On day 0 and day 5 of the therapy, mononuclear cells (MNC) were Materials and Methods Ethics statement Institutional Review Board approval and informed consent for sample collection were obtained before collection of all samples. All the procedures specified below were approved by Ethical Committee

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AnIn VitroStudy of Differentiation of Hematopoietic Cells to Endothelial Cells

Cited by 15 publications

References 31 publications

Contrasting Biofunctionalization Strategies for the Enhanced Endothelialization of Biodegradable Vascular Grafts

Contrasting Biofunctionalization Strategies for the Enhanced Endothelialization of Biodegradable Vascular Grafts

Phenotype of bone-marrow mononuclear cells before and after short-time precondition with erythropoietin from patients with ischemic heart failure

Cytokine Production by Circulating Endothelial Progenitor Cells before and after G-CSF Mobilization

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