An <i>in vitro</i> examination of an extracellular matrix scaffold for use in wound healing

Solomon, Denis E.

doi:10.1046/j.1365-2613.2002.00238.x

Cited by 20 publications

(15 citation statements)

References 24 publications

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“…Specifically, dispase treatment removes the lamina densa (rich in type IV collagen), but preserves type VII collagen-containing anchoring fibrils. [52][53][54] However, a study using collagenase treatment showed that the fibronectin matrix of human periodontal ligament cells was maintained in a cell sheet after 2 h. 55 Finally, Lim et al reported the disruption of several ECM ultrastructures with increasing duration of dispase incubation, indicating that it may be possible to reduce the ECM even further using dispase. 56 Thus, our dispase-based partial-lift technique results in a cell sheet with minimal ECM and the advantages of being readily performed, biocompatible, and open for manipulation.…”

Section: Discussionmentioning

confidence: 99%

Characterization of Partially Lifted Cell Sheets

Huang

2014

Tissue Engineering Part A

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There is a need to characterize biomechanical cell-cell interactions, but due to a lack of suitable experimental methods, relevant in vitro experimental data are often masked by cell-substrate interactions. This study describes a novel method to generate partially lifted substrate-free cell sheets that engage primarily in cell-cell interactions, yet are amenable to biological and chemical perturbations and, importantly, mechanical conditioning and characterization. A polydimethylsiloxane (PDMS) mold is used to isolate a patch of cells, and the patch is then enzymatically lifted. The cells outside the mold remain attached, creating a partially lifted cell sheet. This simple yet powerful tool enables the simultaneous examination of lifted and adherent cells. This tool was then deployed to test the hypothesis that the lifted cells would exhibit substantial reinforcement of key cytoskeletal and junctional components at cell-cell contacts, and that such reinforcement would be enhanced by mechanical conditioning. Results demonstrate that the mechanical strength and cohesion of the substrate-free cell sheets strongly depend on the integrity of the actomyosin cytoskeleton and the cell-cell junctional protein plakoglobin. Both actin and plakoglobin are significantly reinforced at junctions with mechanical conditioning. However, total cellular actin is significantly diminished on dissociation from a substrate and does not recover with mechanical conditioning. These results represent a first systematic examination of mechanical conditioning on cells with primarily intercellular interactions.

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Section: Discussionmentioning

confidence: 99%

Characterization of Partially Lifted Cell Sheets

Huang

2014

Tissue Engineering Part A

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“…A skin segment will be broken down into its cellular compartments with a singular enzymatic use to achieve separation of the epidermis from the dermis. This author has described full details of these methods in previous publications [4] [12].…”

Section: Conceptmentioning

confidence: 99%

“…This has not been attempted in any study thus far and will provide hopefully, an accelerated means of restoring the epithelial barrier. If a delay is sought, for example, to wait until wound exudate has ceased or cultured, it is known that the epidermal rosettes do maintain their structural integrity in vitro as was clearly observed when a co-culture of epidermal rosettes, dermal microvascular endothelial cells and dermal fibroblasts was attempted [12]. They do not shed their load of cells onto a plastic surface; only onto a cell matrix [4].…”

Section: Epidermismentioning

confidence: 99%

“…Of course, results will need to be evaluated to determine whether the procedure needs tweaking, meaning that the treatment interval needs to be extended. However, this would now necessarily involve using P 1 dermal cells, obtained by using 5 mM EDTA/DPBS (pH 7.4) as previously described [12] (Ethylene diamine tetra-acetic acid/Dulbecco's phosphate-buffered saline) or using a cell scraper which will yield a dermal fibroblast cell cluster population plus fragments of their extracellular matrix (ECM). Great care will have to be taken in the choice of a plastic pipette with a sufficiently wide tip to insure that the structural integrity of the cell clusters are maintained.…”

Section: E Solomon Open Journal Of Regenerative Medicinementioning

confidence: 99%

“…Since the dermal fibroblasts will be lying in the patient's own plasma awaiting transplantation, the plasma will become "conditioned" by their secretions (ECM components and fibroblast growth factors). Table I [12] lists some of those secretions. Also, human blood plasma is more than an ordinary fluid; it exhibits viscoelastic behaviour (see Brust M. et al, 2013) [20].…”

Section: Notesmentioning

confidence: 99%

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Tackling Post-Operative Cutaneous Scarring with Autologous Cell Therapy

Solomon¹

2017

OJRM

Self Cite

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Regenerative medicine has brought about refreshing new thinking about age old problems. However, some problems remain mostly untouched and are not being addressed. A point in question is the track of scar tissue left behind post-operatively, which reveals the surgeon's line of invasive incision. This confers on the patient an adverse psychological reminder and burden for the rest of his/her life. Most patients cannot afford corrective plastic surgery to ameliorate this skin defect. This paper seeks to ask whether biomedical scientists could play a role in arriving at a more pleasing cosmetic result, using a simple cell culture procedure of isolating un-manipulated autologous primary epidermal and dermal cells from a small skin tissue segment in close proximity to the surgeon's incision line.

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Co‐electrospun poly(lactide‐co‐glycolide), gelatin, and elastin blends for tissue engineering scaffolds

Mondrinos

Chen

et al. 2006

J Biomedical Materials Res

323

185

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In this study, we describe composite scaffolds composed of synthetic and natural materials with physicochemical properties suitable for tissue engineering applications. Fibrous scaffolds were co-electrospun from a blend of a synthetic biodegradable polymer (poly(lactic-co-glycolic acid), PLGA, 10% solution) and two natural proteins, gelatin (denatured collagen, 8% solution) and alpha-elastin (20% solution) at ratios of 3:1:2 and 2:2:2 (v/v/v). The resulting PLGA-gelatin-elastin (PGE) fibers were homogeneous in appearance with an average diameter of 380 +/- 80 nm, which was considerably smaller than fibers made under identical conditions from the starting materials (PLGA, 780 +/- 200 nm; gelatin, 447 +/- 123 nm; elastin, 1060 +/- 170 nm). Upon hydration, PGE fibers swelled to an average fiber diameter of 963 +/- 132 nm, but did not disintegrate. Importantly, PGE scaffolds were stable in an aqueous environment without crosslinking and were more elastic than those made of pure elastin fibers. To investigate the cytocompatibility of PGE, we cultured H9c2 rat cardiac myoblasts and rat bone marrow stromal cells (BMSCs) on fibrous PGE scaffolds. We found that myoblasts grew equally as well or slightly better on the scaffolds than on tissue-culture plastic. Microscopic evaluation confirmed that myoblasts reached confluence on the scaffold surfaces while simultaneously growing into the scaffolds. Histological characterization of the PGE constructs indicated that BMSCs penetrated into the center of scaffolds and began proliferating shortly after seeding. Our results suggest that fibrous scaffolds made of PGE and similar biomimetic blends of natural and synthetic polymers may be useful for engineering soft tissues, such as heart, lung, and blood vessels.

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An in vitro examination of an extracellular matrix scaffold for use in wound healing

Cited by 20 publications

References 24 publications

Characterization of Partially Lifted Cell Sheets

Characterization of Partially Lifted Cell Sheets

Tackling Post-Operative Cutaneous Scarring with Autologous Cell Therapy

Co‐electrospun poly(lactide‐co‐glycolide), gelatin, and elastin blends for tissue engineering scaffolds

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